N-[(2,4-DIFLUOROPHENYL)METHYL]-6-HYDROXY-3-METHYL-5,7-DIOXO-2,3,5,7,11,11a-HEXAHYDRO[1,3]OXAZOLO[3,2-a]PYRIDO[1,2-d]PYRAZINE-8-CARBOXAMIDE HAVING HIV INTEGRASE INHIBITORY ACTIVITY

ABSTRACT

The present invention is to provide a novol compound (I) shown below, having the anti-virus activity; particularly the HIV integrase inhibitory activity, and a drug containing the same, particularly an anti-HIV drug, as well as a process and an intermediate thereof. 
     
       
         
         
             
             
         
       
     
     (wherein
         Z 1  is NR 4 ;   R 1  is hydrogen or lower alkyl;   X′ is a single bond, a hetoro atom group selected from O, S, SO, SO 2  and NH, or lower alkylene or lower alkenylene in which the hetero atom group may intervene;   R 2  is optionally substituted aryl;   R 3  is hydrogen, a halogen, hydroxy, optionally substituted lower alkyl etc; and   R 4  and Z 2  part taken together forms a ring, to form a polycyclic compound; including e.g., a tricyclic or tetracyclic compound.

TECHNICAL FIELD

The present invention relates to novel compounds possessing an antiviral activity, in detail polycyclic carbamoylpyridone derivatives possessing an inhibitory activity against HIV integrase and a pharmaceutical composition containing the same, especially an anti-HIV agent.

BACKGROUND ART

Among viruses, human immunodeficiency virus (HIV), a kind of retrovirus, is known to cause acquired immunodeficiency syndrome (AIDS). The therapeutic agent for AIDS is mainly selected from a group of reverse transcriptase inhibitors (e.g., AZT, 3TC) and protease inhibitors (e.g., Indinavir), but they are proved to be accompanied by side effects such as nephropathy and the emergence of resistant viruses. Thus, the development of anti-HIV agents having the other mechanism of action has been disired.

On the other hand, a combination therapy is reported to be efficient in treatment for AIDS because of the frequent emergence of the resistant mutant. Reverse transcriptase inhibitors and protease inhibitors are clinically used as an anti-HIV agent, however agents having the same mechanism of action often exhibit cross-resistance or only an additional activity. Therefore, anti-HIV agents having the other mechanism of action are desired.

Under the circumstances above, an HIV integrase inhibitor has been focused on as an anti-HIV agent having a novel mechanism of action (Ref: Patent Documents 1 and 2). As an anti-HIV agent having such a mechanism of action, known are carbamoyl-substituted hydroxypyrimidinone derivative (Ref: Patent Documents 3 and 4) and carbamoyl-substituted hydroxypyrrolidione derivative (Ref: Patent Document 5). Further, a patent application concerning carbamoyl-substituted hydroxypyridone derivative has been filed (Ref: Patent Document 6, Example 8).

Other known carbamoylpyridone derivatives include 5-alkoxypyridine-3-carboxamide derivatives and γ-pyrone-3-carboxamide derivatives, which are a plant growth inhibitor or herbicide (Ref: Patent Documents 7-9).

Other HIV integrase inhibitors include N-containing condensed cyclic compounds (Ref: Patent Document 10).

-   [Patent Document 1]WO03/0166275 -   [Patent Document 2]WO2004/024693 -   [Patent Document 3]WO03/035076 -   [Patent Document 4]WO03/035076 -   [Patent Document 5]WO2004/004657 -   [Patent Document 6]JP Patent Application 2003-32772 -   [Patent Document 7]JP Patent Publication 1990-108668 -   [Patent Document 8]JP Patent Publication 1990-108688 -   [Patent Document 9]JP Patent Publication 1990-96506 -   [Patent Document 10]WO2005/016927

DISCLOSURE OF INVENTION Problem to be Solved by the Invention

The development of a novel integrase inhibitor has been desired.

Means to Solve the Problem

The present inventors have intensively studied to find that a novel polycyclic carbamoylpyridone derivative possesses a potent HIV integrase inhibitory activity.

Moreover, the present inventors have discovered that a compound of the present invention and a pharmaceutical composition containing the same are useful as an antiviral agent, an antiretroviral agent, an anti-HIV agent, an anti-HTLV-1 (Human T cell leukemia virus type 1) agent, an anti-HIV (Feline immunodeficiency virus) agent or an anti-SIV (Simian immunodeficiency virus) agent, especially an anti-HIV agent or anti-AIDS agent, to accomplish the present invention shown below.

-   (1) A compound of the formula:

(wherein,

Z¹ is NR⁴;

R⁴ is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, hydroxy, optionally substituted amino, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened by a heteroatom group selected from CO, O, S, SO, SO₂, NR^(a) (R^(a) is hydrogen or lower alkyl), —N═ and ═N—)), O or CH₂;

Z² is optionally substituted lower alkylene or optionally substituted lower alkenylene, each may be intervened by a heteroatom group selected from O, S, SO, SO₂, NR⁵ (R⁵ is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, hydroxy or optionally substituted amino, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened by a heteroatom group selected from CO, O, S, SO, SO₂, NR⁵ (R⁵ is selected independently from the same substituent group as R⁴), —N═ and ═N—)), —N═ or ═N—

R¹ is hydrogen or lower alkyl;

X is a single bond, a heteroatom group selected from O, S, SO, SO₂ and NH, or lower alkylene or lower alkenylene each may be intervened by the heteroatom;

R² is optionally substituted aryl;

R³ is hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy or optionally substituted amino;

R⁴ and Z² part taken together forms a ring, where the compound (I) is represented by the following formula (I-1), or (I-11):

(wherein,

A ring is optionally substituted heterocycle;

R¹⁴ and R^(X) are independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened by a heteroatom group selected from O, S, SO, SO₂, NR⁵ (R⁵ is selected independently from the same substituent group as R⁴), —N═ and ═N—), hydroxy, optionally substituted amino, optionally substituted lower alkyl carbonyl, optionally substituted cycloalkylcarbonyl, optionally substituted cycloalkyl lower alkyl carbonyl, optionally substituted lower alkoxy carbonyl, optionally substituted arylcarbonyl, optionally substituted aryl lower alkyl carbonyl, optionally substituted aryloxycarbonyl, optionally substituted heterocyclecarbonyl, optionally substituted heterocycle lower alkyl carbonyl, optionally substituted heterocycleoxy carbonyl or optionally substituted aminocarbonyl;

a broken line represents the presence or absence of a bond, provided that when the broken line represents the presence of a bond, R^(X) is not present;

R¹ is hydrogen or lower alkyl;

X is a single bond, a heteroatom group selected from O, S, SO, SO₂ and NH, or lower alkylene or lower alkenylene each may be intervened by the heteroatom group;

R² is optionally substituted aryl;

R³ is hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy or optionally substituted amino)

(wherein,

D ring is optionally substituted heterocycle;

R¹ is hydrogen or lower alkyl;

X is a single bond, a heteroatom group selected from O, S, SO, SO₂ and NH, or lower alkylene or lower alkenylene each may be intervened by the heteroatom group;

R² is optionally substituted aryl;

R³ is hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy or optionally substituted amino)), its pharmaceutically acceptable salt, or solvate thereof.

-   (2) A compound according to the above (1), pharmaceutically     acceptable salt, or solvate thereof, wherein R¹ is hydrogen. -   (3) A compound according to the above (1), pharmaceutically     acceptable salt, or solvate thereof, wherein X is lower alkylene; R²     is phenyl or phenyl substituted with at least halogen. -   (4) A compound according to the above (1), pharmaceutically     acceptable salt, or solvate thereof, wherein R³ is hydrogen,     halogen, hydroxy, lower alkyl, lower alkenyl, lower alkoxy, lower     alkenyloxy or optionally substituted amino. -   (5) A compound according to the above (1), pharmaceutically     acceptable salt, or solvate thereof, wherein R³ is hydrogen. -   (6) A compound according to the above (1), pharmaceutically     acceptable salt, or solvate thereof, wherein RI is hydrogen or lower     alkyl; X is lower alkylene; R² is phenyl or phenyl substituted with     at least halogen; R³ is hydrogen, halogen, hydroxy, lower alkyl,     lower alkenyl, lower alkoxy, lower alkenyloxy or optionally     substituted amino. -   (7) A compound of the formula:

(wherein,

A ring is optionally substituted heterocycle;

R¹⁴ and R^(X) are independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened by a heteroatom group selected from O, S, SO, SO₂, NR⁵ (R⁵ is selected independently from the same substituent group as R⁴), —N═ and ═N—), hydroxy, optionally substituted amino, optionally substituted lower alkyl carbonyl, optionally substituted cycloalkylcarbonyl, optionally substituted cycloalkyl lower alkyl carbonyl, optionally substituted lower alkoxy carbonyl, optionally substituted arylcarbonyl, optionally substituted aryl lower alkyl carbonyl, optionally substituted aryloxycarbonyl, optionally substituted heterocyclecarbonyl, optionally substituted heterocycle lower alkyl carbonyl, optionally substituted heterocycleoxy carbonyl or optionally substituted aminocarbonyl;

a broken line represents the presence or absence of a bond, provided that when the broken line represents the presence of a bond, R^(X) is not present;

R¹ is hydrogen or lower alkyl;

X is a single bond, a heteroatom group selected from O, S, SO, SO₂ and NH, or lower alkylene or lower alkenylene each may be intervened by the heteroatom group;

R² is optionally substituted aryl;

R³ is hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy or optionally substituted amino), its pharmaceutically acceptable salt, or solvate thereof

-   (8) A compound according to the above (7), pharmaceutically     acceptable salt, or solvate thereof, wherein RI is hydrogen or lower     alkyl; X is lower alkylene; R² is phenyl or phenyl substituted with     at least halogen; R³ is hydrogen, halogen, hydroxy, lower alkyl,     lower alkenyl, lower alkoxy, lower alkenyloxy or optionally     substituted amino. -   (9) A compound according to the above (7), pharmaceutically     acceptable salt, or solvate thereof, wherein a broken line     represents the absence of a bond, -   (10) A compound according to the above (7), pharmaceutically     acceptable salt, or solvate thereof, wherein R^(X) is hydrogen; R¹⁴     is hydrogen or optionally substituted lower alkyl. -   (11) A compound according to the above (7), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is an optionally     substituted and optionally condensed 5- to 7-membered heterocycle     containing 1 to 2 hetero atom(s). -   (12) A compound of the formula:

(wherein,

A ring is an optionally substituted and optionally condensed 5- to 7-membered heterocycle containing 1 to 2 hetero atom(s);

the stereochemistry of an asymmetric carbon represented by * shows R- or S-configuration, or a mixture thereof;

R¹⁴ and R^(X) are independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened by a heteroatom group selected from O, S, SO, SO₂, NR⁵ (R⁵ is selected independently from the same substituent group as R⁴), —N═ and ═N—), hydroxy, optionally substituted amino, optionally substituted lower alkyl carbonyl, optionally substituted cycloalkylcarbonyl, optionally substituted cycloalkyl lower alkyl carbonyl, optionally substituted lower alkoxy carbonyl, optionally substituted arylcarbonyl, optionally substituted aryl lower alkyl carbonyl, optionally substituted aryloxycarbonyl, optionally substituted heterocyclecarbonyl, optionally substituted heterocycle lower alkyl carbonyl, optionally substituted heterocycleoxy carbonyl or optionally substituted aminocarbonyl;

R⁸ is hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy or optionally substituted amino), its pharmaceutically acceptable salt, or

R¹ is hydrogen or lower alkyl;

R is independently selected from halogen and Substituent group S1;

Substituent group S1(: optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue, or lower alkyl substituted with optionally substituted phosphoric acid residue (wherein the lower alkyl may be intervened with a heteroatom group(s) selected from CO, O, O, S, SO, SO₂, NR^(a) (R^(a) is hydrogen or lower alkyl), —N═ and ═N—), lower alkoxy lower alkyl, amino lower alkyl optionally substituted with mono- or di- lower alkyl, halogenated lower alkyl, lower alkoxy, carbamoyl optionally substituted with mono- or di- lower alkyl, optionally substituted lower alkyl sulfonyl amino, halogenated lower alkoxy, hydroxy lower alkyl)

m is an integer of 0 to 3, its pharmaceutically acceptable salt, or solvate thereof.

-   (13) A compound according to the above (12), pharmaceutically     acceptable salt, or solvate thereof, wherein R^(X) and R¹⁴ are     independently hydrogen or optionally substituted lower. -   (14) A compound according to the above (12), pharmaceutically     acceptable salt, or solvate thereof, wherein R^(X) and R¹⁴ are     hydrogens. -   (15) A compound according to the above (12), pharmaceutically     acceptable salt, or solvate thereof, wherein R³ is hydrogen. -   (16) A compound according to the above (12), pharmaceutically     acceptable salt, or solvate thereof, wherein m is 0, or 1 to 3 and     at least one of R is halogen. -   (17) A compound according to the above (7) or (12), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is any one of     the following:

(wherein, R²⁰ to R⁴⁰ are each independently a group selected from Substituent group S2, or any two groups of R²⁰ to R⁴⁰, which bonds to the same carbon atom, taken together with the carbon atom, may form an optionally substituted carbocyle or optionally substituted heterocycle, or each combination of (R²⁰ and R²²), (R²³ and R²⁴), (R²⁵ and R²⁶), (R²⁷ and R²⁹), (R³⁰ and R³¹), (R³² and R³⁴), (R³⁵ and R³⁶), (R³⁷ and R³⁸), and (R³⁹and R⁴⁰), taken together with the neighboring atom, may form an optionally substituted carbocyle or optionally substituted heterocycle.

Substituent group S2: hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocycle, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, hydroxy, optionally substituted amino, optionally substituted lower alkylcarbonyl, optionally substituted cycloalkylcarbonyl, optionally substituted cycloalkyl lower alkylcarbonyl, optionally substituted lower alkoxycarbonyl, optionally substituted arylcarbonyl, optionally substituted aryl lower alkylcarbonyl, optionally substituted aryl oxycarbonyl, optionally substituted heterocyclecarbonyl, optionally substituted heterocycle lower alkylcarbonyl, optionally substituted heterocycleoxycarbonyl, optionally substituted aminocarbonyl, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue, or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened with a heteroatom group(s) selected from CO, O, S, SO, SO₂, NR⁵ (R⁵ is independently selected from the same Substituent group as R⁴), —N═ and ═N—) the stereochemistry of an asymmetric carbon represented by * shows R- or S-configuration, or a mixture thereof)

-   (18) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein R²⁰ to R⁴⁰ are each     independently hydrogen or substituted lower alkyl, or any two groups     of R²⁰ to R⁴⁰, which bonds to the same carbon atom, taken together     with the carbon atom, may form an optionally substituted 3- to     7-membered carbocyle or optionally substituted 3- to 7-membered     heterocycle, or each combination of (R²⁰ and R²²), (R²¹ and R²⁴),     (R²⁶ and R²⁶), (R²⁷ and R²⁹), (R³⁰ and R³¹), (R³² and R³⁴), (R³⁵ and     R³⁶), (R³⁷ and R³⁸), and (R³⁹ and R⁴⁰), taken together with the     neighboring atom, may form an optionally substituted 5- to     7-membered carbocyle or optionally substituted 5- to 7-membered     heterocycle. -   (19) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-1); one of R²⁰ to R²⁵ is optionally substituted     lower alkyl and the others are hydrogens. -   (20) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-1); one of (R²⁰ and R²²), (R²³ and R²⁴), and (R²⁵     and R²⁶), taken together with the neighboring atom, may form an     optionally substituted 5- to 7-membered carbocyle or optionally     substituted 5- to 7-membered heterocycle. -   (21) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-1); Z═NR²⁶, and R²⁵ and R²⁶ taken together with     the neighboring atom may form an optionally substituted 5- to     7-membered heterocycle. -   (22) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-2); one of R²⁷ to R³⁰ is optionally substituted     lower alkyl and the others are hydrogens. -   (23) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-2); one of (R²⁷and R²⁹) and (R³⁰ and R³¹), taken     together with the neighboring atom, may form an optionally     substituted 5- to 7-membered carbocyle or optionally substituted 5-     to 7-membered heterocycle. -   (24) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-2); Z═NR³¹, and R³⁰ and R³¹ taken together with     the neighboring atom may form an optionally substituted 5- to     7-membered heterocycle. -   (25) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-3); one of R³² to R³⁹ is optionally substituted     lower alkyl and the others are hydrogens. -   (26) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-3); one of (R³² and R³⁴), (R³⁵ and R³⁶), (R³⁷ and     R³⁸),and (R³⁹ and R⁴⁰), taken together with the neighboring atom,     may form an optionally substituted 5- to 7-membered carbocyle or     optionally substituted 5- to 7-membered heterocycle. -   (27) A compound according to the above (17), pharmaceutically     acceptable salt, or solvate thereof, wherein A ring is a ring     represented by (A-3); Z═NR⁴⁰, and R³⁹ and R⁴⁹ taken together with     the neighboring atom may form an optionally substituted 5- to     7-membered heterocycle. -   (28) A compound according to the above (12), pharmaceutically     acceptable salt, or solvate thereof, wherein R^(X) is hydrogen; R¹⁴     is hydrogen or optionally substituted lower; R³ is hydrogen; m is 1     to 3 and at least one of Rs is halogen; A ring is a ring described     in Claim 17. -   (29) A compound according to the above (12), pharmaceutically     acceptable salt, or solvate thereof, wherein R^(X) is hydrogen; R¹⁴     is hydrogen; R³ is hydrogen; m is 0, or 1 to 3 and at least one of     Rs is halogen; A ring is a ring described in Claim 17; R²⁰ to R⁴⁰     are each independently hydrogen or substituted lower alkyl, or any     two groups of R²⁰ to R⁴⁰, which bonds to the same carbon atom, taken     together with the carbon atom, may form an optionally substituted 3-     to 7-membered carbocyle or optionally substituted 3- to 7-membered     heterocycle, or each combination of (R²⁰ and R²²), (R²³ and R²⁴),     (R²⁵ and R²⁶), (R²⁷ and R²⁹), (R³⁰ and R³¹), (R³² and R³⁴), (R³⁵ and     R³⁸), (R³⁷ and R³⁸), and (R³⁹ and R⁴⁰), taken together with the     neighboring carbon atom, may form an optionally substituted 5- to     7-membered carbocyle or optionally substituted 5- to 7-membered     heterocycle. -   (30) A compound of the formula:

(wherein,

D ring is optionally substituted heterocycle;

R¹ is hydrogen or lower alkyl;

X is a single bond, a heteroatom group selected from O, S, SO, SO₂ and NH, or lower alkylene or lower alkenylene each may be intervened by the heteroatom group;

R² is optionally substituted aryl;

R³ is hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy or optionally substituted amino), pharmaceutically acceptable salt, or solvate thereof

-   (31) A compound selected from the group consisting of: -   (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (4aR,13aS)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyriclo[1,2-a]pyrrolo[1,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide; -   (3aS,13aS)-N-[(2,4-Difluorophonyl)methyl]-8-hydroxy-7,9-dioxo-1,2,3,3a,4,5,7,9,13,13a-decabydropyrido[[1,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide; -   (4aS,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide; -   (4a8,13aR)-N-[(4-Fluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-oct     ahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-3-(phenylmethyl)-2,3,5,     7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3aS,13aS)-N-[(4-Fluorophenyl)methyl]-8-hydroxy-7,9-dioxo-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(1S)-1-methylpropyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-     a]pyrido[1,2-d]pyrazine8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3     -methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-     a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(4-Fluorophenyl)methyl]6-hydroxy-3-methyl-5,7-     dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2a]pyrido[1,2-d]pyrazino-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-3-(1,1-dimethylethyl)-6-hydroxy-5,7-dioxo-2,3,5,7     1,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-3-(1,1-Dimethylethyl)-N-[(4-fluorophenyl)methyl]-6     -hydroxy-5,7-dioxo2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-3-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(hydroxymethy)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (2S,3R—N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6hydroxy-5,7-dioxo-3-(phenylmethyl)-2,3,5,7,     11,11a-hexhydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8     -carboxamide; -   (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(2-methylpropyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (5aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide; -   (2s,3S)—N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3     -[(methyloxy)methyl]-5,7-dioxo-2-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide;     p0     (3S,11aR)-3-(Cyclohexylmethyl)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7     -dioxo-2,3,6,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(1-methylethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (5aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-5a,6a,7,11,13,14a-hexahydro-5H-indeno[1′,2′:4,5][1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-10-carboxamide; -   (2S,3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3-diphenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-     a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (2S,3R,11aR)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2, 3     -diphenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(1-methylethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N[(2,4-Difluorophenyl)methyl]-6-     hydroxy-3-[2-(methylthio)ethyl]-5,7-dioxo-2,3,5,7,11,11     a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide; -   (3S,11aR)-N[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[2-(methylsulfonyl)ethyl]-5,7-dioxo-2,3,     5,7,11,11a-hexahydro[1,3]oxazolo[3,2a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)     methyl]-6-hydroxy-3-(1H-indol-3-ylmethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide; -   (4R,12aR)-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-oetahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4R,12aR)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(2,     4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-oetahydropyrido[1′,2′:4,5]pyrazino[1,2a]pyrimidine-9-carboxamide; -   (4S,12aS)-1-(Cyclopropylmethyl)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-1-(2-furanylmethyl)-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(1,3-thiazol-2-ylmethyl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamido; -   (4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a,     7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide; -   (4aR,6aR,14aS)-N-[(4-Fluorophenyl)methyl]-12-hydroxy-11,18-dioxo-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide -   (3S,4aR,6aR,14aS)-N     [(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-3-phenyl-1,3,11,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide; -   (4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-(2-methylpropyl)-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide; -   (6aR,7aS,11aS)-N-[(2,4-Difluorophenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide; -   (6aS,7aS,11aS)-N-[(2,4-Difluorophenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide; -   (5aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide; -   (4aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-9-hydroxy-8,10-dioxo-2,3,4,4a,5,6,8,10,14,14a-decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide; -   (4R,12aR)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(3-methylbutyl)-6,8-dioxo-1,2,8,4,6,8,     12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-     a]pyrimidine-9-carboxamide; -   (4S,12aS)-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine9-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(3-methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(3-pyridinyl     methyl)-1,2,8,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-1- Cyclopropyl-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4     -methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (3aS,5aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-5-(2-methylpropyl)-10,12-dioxo-2,3,3a,4,5,5a,6,10,12,13a-decahydro-1H-cyclopenta[e]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-3-ethyl-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-[2-(4-morpholinyl)ethyl]-11,13-dioxo-1,2,3,21,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide; -   (3aR,5aR,13aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,3a,4,5a,6,10,12,13a-decahydrocyclopenta[d]pyrido[1′,2′:4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide; -   (4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-methyl-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide; -   (4aS,6aS,14aS)-N     [(2,4-Difluorophenyl)methyl]-12-hydroxy-6-[2-(methyloxy)ethyl]-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide; -   (4aS,6aS,14aS)-6-[2-(Acetylamino)ethyl]-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-3-ethyl-6     hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-3-Butyl-N     [(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(4-hydroxyphenyl)methyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (4S,12aS)-1-Cyclobutyl-N-[(2,     4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2a]pyrimidine-9-carboxamide; -   (4S,¹²aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-(2-hydroxyethyl)-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10carboxamide; -   (4aS,6aS,14aS)-6-Cyclopropyl-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2a]quinazoline-10-carboxamide; -   (4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-     dioxo-6-[2-(1-pyrrolidinyl)ethyl]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide; -   (4aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-9-hydroxy-8,10-dioxo-2,3,4,4a,5,6,8,10,14,14a-decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide; -   (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12,12a-oetahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-1-Cyclobutyl-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12a8)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-     dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-     carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,8,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-     a]pyrimidine-9-carboxamide; -   (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide;     enantiomers thereof; diastereomers thereof; mixtures of enantiomers     thereof; mixtures of diastereomers thereof; mixtures of enantiomers     and diastereomers thereof; and pharmaceutically acceptable salts     thereof. -   (32) A compound selected from the group consisting of: -   (4aS,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,     9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide; -   (4aS,13aR)-N-[(4-Fluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine8-carboxamide; -   (3S,     11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(1S)-1-methylpropyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide; -   (3S,11aR)-N-[(4-Fluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide; -   (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4S,12aS)-1-(Cyclopropylmethyl)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide; -   (4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a,     7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide; -   (4a,R,6aR,14aS)-N-[(4-Fluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide; -   4S,9aR)-5-Hydroxy-4-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,     8a-diaza-anthracene-7-carboxylic acid 2,4,-difluoro-benylamide; -   4R,9aS)-5-Hydroxy-4-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic     acid 2,4,-difluoro-benylamide; -   2R,9aS)-5-Hydroxy-2-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic     acid 4-fluoro-benylamide; enantiomers thereof; diastereomers     thereof; mixtures of enantiomers thereof; mixtures of diastereomers     thereof; mixtures of enantiomers and diastereomers thereof; and     pharmaceutically acceptable salts thereof. -   (33) A compound according to the above (31) or (32) wherein the     pharmaceutically acceptable salt is a sodium salt. -   (34) A pharmaceutical composition comprising a compound according to     any one of the above (1) to (33), or a pharmaceutically acceptable     salt, or solvate thereof. -   (35) A pharmaceutical composition according to the above (34), which     is an anti-HIV agent. -   (36) A process for the preparation of a compound of formula (I-20a)

wherein R^(e) is one or two halogen; R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C ₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

wherein R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy; to form a compound of formula (I-20a).

-   (37) A process for the preparation of a compound of formula (I-20b)

wherein R^(o) is one or two halogen; R^(z) is C₆₋₁₄alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁶⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

wherein R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy;

-   to form a compound of formula (I-20b). -   (38) A process for the preparation of a compound of formula (I-21a)

wherein R^(a) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

to form a compound of formula (I-21a).

-   (39) A process for the preparation of a compound of formula (I-21b)

wherein R^(o) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(o) is one or two halogen; R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

to form a compound of formula (I-21b).

-   (40) A process for the preparation of a compound of formula (I-22a)

wherein R^(e) is one or two halogen; and P¹ is C₈₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

to form a compound of formula (I-22a),

-   (41) A process for the preparation of a compound of formula (I-22b)

wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

to form a compound of formula (I-22b).

-   (42) A process for the preparation of a compound of formula (I-23a)

wherein R^(o) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

to form a compound of formula (I-23a).

-   (43) A process for the preparation of a compound of formula (I-23b)

wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C₁₋₈alkyl;

-   with a compound of the formula

to form a compound of formula (I-23b).

-   (44) A process for the preparation of a compound of formula (I-24a)

wherein R^(e) is one or two halogen; R_(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; and R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

wherein R^(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may b e optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

-   to form a compound of the formula (I-24a). -   (45) A process for the preparation of a compound of formula (I-24b)

wherein R^(o) is one or two halogen; R^(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a compound of the formula

wherein R^(z) is C₁₋₈alkyl; and R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₈₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may he optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

-   to form a compound of the formula (I-24b). -   (46) A process for the preparation of a racemic compound of formula     (I-25)

wherein R^(e) is one or two halogen; R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl; and P¹ is is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; and R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a racemic compound of the formula

wherein R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

-   to form a racemic compound of the formula (I-25). -   (47) A process for the preparation of a racemic compound of formula     (I-26)

wherein R^(e) is one or two halogen; R^(z1) is hydrogen, C₃₋₆cycloa.lkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(e) is one or two halogen; R⁵⁰ is C ₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a racemic compound of the formula

wherein R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₆alkyl;

-   to form a racemic compound of formula (I-26). -   (48) A process for the preparation of a racemic compound of formula     (I-27)

wherein R^(e) is halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   comprising condensing a compound of the formula

wherein R^(o) is one or two halogen; R⁵⁰ is C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

-   with a racemic compound of the formula

to form a racemic compound of formula (I-27).

-   (49). A compound of formula (I20a) described in above (36). formula     (I-20b) described in above (37), formula (I-21a) described in above     (38), formula (I-23,b) described in above (39), formula (I-22a)     described in above (40), formula (I-22b) described in above (41),     formula (I-23a) described in above (42), formula (I-23h) described     in above (43), formula (I-24a) described in above (44), formula     (I-24b) described in above (45), formula (I-25) described in above     (46), formula (I-26) described in above (47), or formula (I-27)     described in above (48), or a pharmaceutically acceptable salt     thereof. -   (50) A compound of formula (I-20a) described in above (36), formula     (I-20b) described in above (37), formula (I-21a) described in above     (38), formula (I-21b) described in above (39), formula (I-22a)     described in above (40), formula (I-22b) described in above (41),     formula (I-23a) described in above (42), formula (I-23b) described     in above (43), formula (I24a) described in above (44), formula     (I-24b) described in above (45), formula (I-25) described in above     (46), formula (I-26) described in above (47), or formula (I-27)     described in above (48), or a pharmaceutically acceptable salt     thereof, wherein each P¹ is hydrogen,

The present invention further provides a pharmaceutical composition containing any of the compounds shown above, a pharmaceutically acceptable salt or a solvate thereof, especially an anti-HIV agent.

Effect of the Invention

The present invention compounds possess an integrase inhibitory activity and/or a cell-growth inhibitory activity against virus, especially HIV. Accordingly, they are useful for the prevention or treatment of various diseases mediated by integrase or virus infection diseases (e.g., AIDS). The present invention further provides a process for preparing a diastereomer, a mixture thereof, or racemate.

PREFERRED EMBODIMENT OF THE INVENTION

The terms used. herein are explained below, Each term, alone or in combination with another tern, means as follows.

“Lower alkylene” means a straight or branched C1 to C6 alkylene such as methylene, ethylene, trimethylene, npropylene, tetramethylene, ethylethylene, pentamethylene, or hexamethylene, preferably C1 to C4 straight alkylene such as. methylene, ethylene, trimethylene, and tetramethylene, more preferably methylene or ethylene.

“Lower alkenylene” means a straight or branched C2 to C6 alkenylene, which consists of the above “Lower alkylene” having one or more double bonds, such as vinylene, propylene, or butenylene, preferably a straight C2 to C3 alkenylene such as vinylene or propylene.

“Lower alkyl” means a straight or branched C1 to C10 alkyl such as methyl, ethyl, n-propyl, t-butyl, isobutyl, sec-butyl, n-pentyl, and n-hexyl, and preferred is C1 to C3 alkyl, more preferred is methyl, ethyl or n-propyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, n-hexyl, isohexyl, n-heptyl, n-octyl, nnonyl, and ndesyl, preferably C1 to C6 lower alkyl, more preferably C1 to C4 lower alkyl such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, n-hexyl, and isohexyl.

When lower alkyl is intervened with “—N═” or “═N—”, the lower alkyl may have a double bond to form —CH₂—N═CH₂, —CH═N—CH₈ etc.

“Alkenyl” means a straight or branched C2 to C8 alkenyl, which consists of the above “alkyl” having one or more double bonds, such as vinyl, 1-propenyl, 2-propenyl, 1-butenyl, 2-butenyl, 3-butenyl, 1,3-butadienyl, and 3-methyl-2-butenyl, preferably C2 to C6 alkenyl, and more preferably C2 to C4 alkenyl.

“Lower alkenyloxy” means oxy attached to the above lower alkenyl, such as vinyloxy, 1-propenyloxy, 2-propenyloxy, 1-butenyloxy, 2-butenyloxy, 3-butenyloxy, 1,3-butadienyloxy, and 3-methyl-2-butenyloxy.

“Cycloalkyl” means C3 to C8 cyclic saturated hydrocarbon, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclopentyl, and cyclooctyl, preferably C3 to C6 cycloalkyl.

“Cycloalkyl lower alkyl” means lower alkyl substituted with the above cycloalkyl, such as cyclopropylmethyl, cyclopropylethyl, cyclobutylmethyl, cyclopentylmethyl, cyclohexylmethyl, and cyclohexylethyl, and preferably C8 to C6 cycloalkyl lower alkyl.

“Aryl” means monocyclic aromatic hydrocarbon (e.g., phenyl) and polycyclic hydrocarbon (e.g., 1-naphthyl,2- naphthyl, 1-anthryl, 2-anthryl, 9-anthryl, 1-phenanthryl, 2 -phenanthryl, 3-phenanthryl, 4-phenanthryl, 9-phenanthryl), preferably phenyl or naphthyl (e.g., 1-napthyl, 2-naphthyl).

“Aralkyl” or “aryl lower alkyl” means the above lower alkyl substituted with 1 to 3 of the above aryl, such as benzyl, diphenylmethyl, triphenylmethyl, phenethyl, 1-napthylmethyl, 2-napthylmethyl, preferably benzyl.

“Aryloxy” means oxy attached to the above aryl, such as 1-naphthyloxy, 2 -naphthyloxy, 1-anthryloxy, 2-anthryloxy, 9-anthryloxy, 1-phenanthryloxy, 2-phenanthryloxy, 3-phenanthryloxy, 4-phenanthryloxy, and 9-phenanthryloxy, preferably phenyloxy or naphthyloxy (e.g., 1-napthyloxy, 2-naphthyloxy).

“Heterocyclic group” means “heteroring” or “heteroaryl”.

“Heteroring” means a non-aromatic ring which has at least one of N, O and/or S in the ring and may be bonded at any substitutable position, preferably 5- to 7-membered ring, such as 1-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, 1-pyrrolidinyl, 2-pyrrolidinyl, 3-pyrrolidinyl, 1-imidazolyl, 2-imidazolyl, 4-imidazolyl, 1-imidazolidinyl, 2-imidazolidinyl, 4-imidazolidinyl, 1-pyrazolinyl, 3-pyrazolinyl, 4-pyrazolinyl, 1-pyrazolidinyl, 3-pyrazolidinyl, 4-pyrazolidinyl, piperidino, 2-piperidyl, 3-piperidyl, 4-piperidyl, 1-piperadinyl, 2-piperadinyl, 2-morpholinyl, 3-morpholinyl, morpholino, and tetrahydropyranyl. The non-aromatic ring is a saturated or unsaturated ring.

“Heteroaryl” means monocyclic aromatic hetero-type ring or condensed aromatic hetero-type ring.

“Monocyclic aromatic hetero-type ring” means a 5to 8-membered aromatic ring, which contains 1 to 4 of O, S, P and/ or N and may be bonded at any substitutable position.

“Condensed aromatic hetero-type ring” means a group wherein an aromatic ring containing 1 to 4 of O, S, P and/ or N is condensed with 1 to 4 of 5- to 8-membered aromatic lines) or the other 5- to 8-membered aromatic heteroring(s).

Examples of “heteroaryl” include furyl (e.g., 2-furyl, 3-furyl), thienyl (e,g., 2-thienyl, 3-thienyl), pyrrolyl (e.g., 1.-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl), imidazolyl (e.g., 1-imidazolyl, 2-imidazolyl, 4-imidazolyl), pyrazolyl (e.g., 1-pyrazolyl, 3-pyrazolyl, 4-pyrazolyl), triazolyl (e.g., 1,2,4-triazole-1-yl, 1,2,4-triazole-3-yl, 1,2,4-triazole-4-yl), tetrazolyl (e.g., 1-tetrazolyl, 2-tetrazolyl, 5-tetrazolyl), oxazolyl (e.g., 2-oxazolyl, 4-oxazolyl, 5-oxazolyl), isoxazolyl (e.g., 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl), thiazolyl (e.g., 2-thiazolyl, 4-thiazolyl, 5-thiazolyl), thiadiazolyl, isothiazolyl (e.g., 3-isothiazolyl, 4-isothiazolyl, 5-isothiazolyl), pyridil(e.g., 2 pyridil, 3-pyridil, pyridazinyl 3-pyridazinyl, 4-pyridazinyl)) pyrimidinyl (e.g., 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl), furazanyl (e.g., 3-furazanyl), pyrazinyl (e.g., 2-pyrazinyl), oxadiazolyl (e.g., 1,3,4-oxadiazole2-yl), benzofuryl (e.g., 2-benzo[b]furyl, 8-benzo[b]furyl, 4-benzo[b]furyl, 5-benzo[b]furyl, 6-benzo[b]furyl, 7-benzo[b]furyl), benzothienyl (e.g., 2-benzo[b]thienyl, 3-benzo[b]thienyl, 4-benzo[b]thienyl, 5benzo[b]thienyl, 6-benzo[b]thienyl, 7-benzo[b]thienyl), benzoimidazolyl (e.g., 1-benzoimidazolyl, 2-benzoimidazolyl, 4-benzoimidazolyl, 5-benzoimidazolyl), dibenzofuryl, benzooxazolyl, quinoxalinyl (e.g., 2-quinoxalinyl, 5-quinoxalinyl, 6-quinoxalinyl), cinnolinyl (e,g., 3-cinnolinyl, 4-cinnolinyl, 5-cinnolinyl, 6-cinnolinyl, 7-cinnolinyl, quinazolinyl (e.g., 2-quinazolinyl, 4-quinazolinyl, 5-quinazolinyl, 6-quinazolinyl, 7-quinazolinyl, 8-quinazolinyl), quinolyl (e.g., 2-quinolyl, 3-quinolyl, 4-quinolyl, 5-quinolyl, 6-quinolyl, 7-quinolyl, 8-quinolyl), phthalazinyl (e.g., 1-phthalazinyl, 5-phthalazinyl, 6-phthalazinyl), isoquinolyl (e.g., 1-isoquinolyl, 3-isoquinolyl, 4-isoquinolyl, 5-isoquinolyl, 6-isoquinolyl, 7-isoquinolyl, 8-isoquinolyl), purinyl, pteridinyl (e.g., 2-pteridinyl, 4-pteridinyl, 6-pteridinyl, 7-pteridinyl), carbazolyl, phenanthridinyl, acridinyl (e.g., 1-acridinyl, 2-acridinyl, 3-acridinyl, 4-acridinyl, 9-acridinyl), indolyl (e.g., 1-indolyl, 2-indolyl, 3-indolyl, 4-indolyl, 5-indolyl, 6-indolyl, 7-indolyl), isoindolyl, phenandinyl (e.g., 1-phenandinyl, 2-phenandinyl) or phenothiadinyl (e.g., 1-phenothiadinyl, 2-phenothiadinyl, 8-phenothiadinyl, 4-phenothiadinyl).

“Heterocycle” means a cycle which can be lead to the above heterocyclic group.

“Heterocyclic group lower alkyl” or “Heterocycle lower alkyl” means lower alkyl substituted with the above heterocyclic group.

“Heterocyclic group oxy” or “Heterocycle oxy” means an oxy attached to the above heterocyclic group.

“Heterocyclic group carbonyl” or “Heterocyclecarbonyl” means a carbonyl attached to the above heterocyclic group

“Lower alkoxy” or “alkoxy” means an oxy attached to the above lower alkyl, such as methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, tert-butoxy.

“Lower alkylcarbonyl”, “cycloalkylcarbonyl”, “cycloalkyl lower alkylcarbonyl”, “lower alkoxyca rbonyl”, “arylcarbonyl”, “aryl lower alkylcarbonyl”, “aryloxycarbonyl”, “heterocyclecarbonyl”, “heterocycle lower alkylcarbonyl”, and “heterocycle oxycarbonyl”, each means a carbonyl attached to the above “lower alkyl”, “cycloalkyl”, “cycloalkyl lower alkyl”, “lower alkoxy”, “aryl”, “aryl lower alkyl”, “aryloxy”, “heterocycle”, “heterocycle lower alkyl”, and “heterocycleoxy”, respectively.

When a substituent(s) is/are present on “optionally substituted lower alkyl”, “optionally substituted cycloalkyl”, “optionally substituted cycloalkyl lower alkyl”, “optionally substituted lower alkenyl”, “optionally substituted lower alkoxy”, “optionally substituted aryl”, “optionally substituted aryl lower alkyl”, “optionally substituted aryloxy”, “optionally substituted aryloxy lower alkyl”, “optionally substituted heterocyle, “optionally substituted heterocyclic group”, “optionally substituted heterocycle lower alkyl”, “optionally substituted. heterocycleoxy”, “optionally substituted lower alkenyloxy”, “optionally substituted lower alkylcarbonyl”, “optionally substituted cycloalkylcarbonyl”, “optionally substituted cycloalkyl lower alkylcarbonyl”, “optionally substituted lower alkoxycarbonyl”, “optionally substituted arylcarbonyl”, “optionally substituted aryl lower alkylcarbonyl”, “optionally substituted aryloxycarbonyl”, “optionally substituted heterocyclecarbonyl”, “optionally substituted heterocycle lower alkylcarbonyl”, “optionally substituted heterocycleoxycarbonyl”, “optionally substituted lower alkylene”, “optionally substituted lower alkenylene”, “optionally substituted phosphoric acid residue”, “optionally substituted carbocycle” or “optionally substituted heterocycle”, each may be substituted with the same or different, 1 to 4 group(s) selected from Substituent group B at any position.

Examples of Substituent group B include hydroxy, carboxy, halogen (F,Cl, Br, I), halo lower alkyl (e.g., CF₃, CH₂CF₃, CH₂CCl₃), halo lower alkoxy (e.g., OCF_(a), OCH₂, CF₃, OCH₂CCl₃), lower alkyl (e.g., methyl, ehtyl, isopropyl, tert-butyl), lower alkenyl (e.g., vinyl), lower alkynyl (e.g., ethynyl), cycloalkyl (e.g., cyclopropyl), cycloalkenyl (e.g., cyclopropenyl), lower alkoxy (e.g., methoxy, ethoxy, propoxy, butoxy), lower alkenyloxy vinyloxy, allyloxy), lower alkoxycarbonyl (e.g., methoxycarbonyl, ethoxycarbonyl, tert-butoxycarbonyl), nitro, nitroso, optionally substituted amino (e.g., alkylamino (e.g., methylamino, ethylamino, dimethylamino), acylamino (e.g., acetylamino, benzoylamino), aralkylamino (e.g., benzylamino, trithylamino), hydroxyamino), azido, aryl (e.g., phenyl), aralkyl (e.g., benzyl), cyano, isocyano, isocyanate, thiocyanate, isothiocyanate, mercapt, alkylthio (e.g., methylthio), alkylsulfonyl (e.g., methansulfonyl, ethansulfonyl), optionally substituted alkylsulfonylamino (e.g., methanesulfonylamino, ethansulfonylamino, N-methylsulfonyl-N′-methylamino), optionally substituted carbamoyl (e.g., alkylca rb a moyl methylcarb a m oyl, ethylcarbamoyl, dimethylcarbamoyl)), sulfamoyl, acyl (e.g., formyl, acetyl), formyloxy, haloformyl, oxal, thioformyl, thiocarboxy, dithiocarboxy, thiocarbamoyl, sulfino, sulfo, sulfoamino, hydrazino, azido, ureido, amizino, quanidino, phthalimide, oxo, phosphoric acid residue, lower alkyl which is substituted with a phosphoric acid residue and may be intervened with a heteroatom group(s), aryl substituted with a phosphoric acid residue, aralkyl substituted with a phosphoric acid residue, hydroxyl lower alkyl, preferably hydroxy, carboxy, halogen(F,Cl,Br,I), halo lower alkyl (e.g., CF₃, CH₂CF₃, CH₂CCl₃), halo lower alkoxy (e.g., OCF₃, OCH₂CF₃, OCH₂CCl₃), lower alkyl (e.g., methyl, ethyl, isopropyl, tert-butyl), lower alkoxy (e.g., methoxy, ethoxy, propoxy, butoxy), optionally substituted amino (e.g., alkylamino (e.g., methylamino, ethylamino, dimethylamino), oxo, or phosphoric acid residue.

Examples of a substituent of “optionally substituted amino” or “optionally substituted carbamoyl” include mono- or di-lower alkyl, lower alkylcarbonyl, lower alkylsulfonyl, optionally substituted lower alkyl (e.g., methyl, ethyl, isopropyl, benzyl, carbarnoylalkyl (e.g., carbamoylmethyl), mono- or di-lower alkylcarbamoyl lower alkyl (e.g., dimethylcarbamoylethyl), hydroxyl lower alkyl, heterocycle lower alkyl (e.g., morpholinoethyl, tetrahydropyranylethyl), alkoxycarbonyl lower alkyl (e.g., ethoxycarbonylmethyl, ethoxycarbonylethyl), mono- or di-lower alkylamino lower alkyl (e.g., dimethylarninoethyl)), lower alkoxy lower alkyl (e.g., methoxyethyl, ethoxymethyl, etboxyethyl, isopropoxyethyl), acyl (e.g., formyl, optionally substituted lower alkylcarbonyl (e.g., acetyl, propionyl, butylyl, isobutylyl, valeryl, isovaleryl, pivaroyl, hexanoyl, octanoyl, methoxyethylcarbonyl, 2,2,2-trifluoroethylcarbonyl, ethoxycarbonylmethylcarbonyl), lower alkoxy lower alkylcarbonyl (e.g., methoxyethylcarbonyl), lower alkylcarbamoyl lower alkylcarbonyl (e.g., methylcarbamoylehtylcarbonyl), alkoxycarbonylacetyl), optionally substituted arylcarbonyl (e.g., benzoyl, toloyl), optionally substituted aralkyl (e.g., benzyl, 4-fluorobenzyl), hydroxy, optionally substituted lower alkylsulfonyl (e.g., methanesulfonyl, ethanesulfonyl, isopropylsulfonyl, 2,2,2-trifluoroethanesulfonyl, benzylsulfbnyl, methoxyethylsulfonyl), lower alkyl, or arylsulfonyl optionally substituted with halogen (e.g., benzenesulfonyl, toluenesulfonyl, 4-fluorobenzenesulfonyl, fluorobenzenesulfonyl), cycloalkyl (e.g., cyclopropyl), aryl optionally substituted with lower alkyl (e.g., phenyl, trithyl), lower alkyl aminosulfonyl (e.g., methylaminosulfonyl, dimethylaminosulfonyl), lower alkylaminocarbonyl (e.g., dimethylaminocarbonyl), lower alkoxycarbonyl (e.g., ethoxycarbonyl), cycloalkylcarbonyl (e.g., cyclopropylcarbonyl, cyclohexylcarbonyl), optionally substituted sulfamoyl (e.g., sulfamoyl, methylsulfamoyl, dimethylsulfamoyl), lower alkylcarbonylamino (e.g., methylcarbonylamino), heterocycle (e.g., morpholino, tetrahydropyranyl), optionally substituted amino (e.g., mono- or di-alkylamino (e.g., dimethylamino), formylamino).

As to amino of “optionally substituted amino”, “optionally substituted carbamoyl”, or “optionally substituted carbamoylcarbonyl”, two substituents on the amino together with the neighboring N atom may form an N-containing heterocycle which optionally contains S and/or O in the ring (preferably 5- to 7-membered ring or saturated ring) and is optionally substituted with oxo or hydroxy. The optional S atom in the ring may be substituted with oxo. The N-containing heterocycle is preferably a 5- or 6-membered ring such as piperadinyl, piperidino, morpholino, pyrrolidino, 2-oxopiperidino, 2-oxopyrrolidino, 4-hydroxymorpholino.

“Phosphoric acid residue” means a group shown of the formula: —PO(OH)₂. “Optionally substituted phosphoric acid residue” means a phosphoric acid residue wherein the OH part and/or a hydrogen of the OH is optionally substituted with a phosphoric acid residue, preferably shown by the formula:

(wherein, R^(A) and R^(B) each is independently OR^(C) or NR^(D)R^(E) (wherein R^(C), R^(D) and R^(E) are each independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heterocyclic group, or R^(D) and R^(E) taken together with the neighboring N atom may form an optionally substituted heterocycle (preferably 5- to 6-membered ring)) or R^(A) and R^(B) taken together with the neighboring P atom may form an optionally substituted heterocycle (preferably 5- to 6-membered ring)).

Preferably, R^(A) and R^(B) are both OR^(C), or one of them is OR^(C) and the other is NR^(D)R^(E), R^(C), R^(D) and R^(E) each is preferably, independently, lower alkyl (e.g., methyl, ethyl).

The optionally substituted heterocycle formed by R^(A) and R^(B) taken together with the neighboring P atom may be the following structure:

(wherein, the broken line means a part of the ring)

Hydroxy substituted with optionally substituted phosphoric acid residue is preferably hydroxy substituted with a phosphoric acid residue substituted with di lower alkyls, and more preferably a group of the formula:

Amino substituted with optionally substituted phosphoric acid residue is preferably amino substituted with a phosphoric acid residue substituted with di lower alkyls, and more preferably a group of the formula:

More Preferable Embodiments

R¹ is hydrogen or lower alkyl, preferably hydrogen.

X is a single bond, a heteroatom group selected from O, S, SO, SO₂ and NH (hereafter also referred to as “M”), or lower alkylene or lower alkenylene each may be intervened by the heteroatom. The term of “intervened by” means the following cases:

-   1) The heteroatom group is present between carbon atoms which     constitutes the alkylene or alkenylene. -   2) The heteroatom group is attached to the N atom of the carbamoyl     group neighboring to X. -   3) The heteroatom group is attached to R² neighboring to X.

The heteroatom group (M) may be the same or different:, and one or more atoms. Examples of that lower alkylene is intervened by a heteroatom group include -M-CH₂—, —CH₂-M-, —CH₂-M-, and —CH₂-M-M-CH₂—.

X is preferably a spacer consisting 1 to 3 joined atoms. X is more preferably lower alkylene or lower alkenylene each may be intervened by a heteroatom group, or O. X is most preferably C1 to C3 alkylene, C2 to C3 alkenylene, or O. Especially preferred is methylene or O.

R² is optionally substituted aryl, preferably phenyl. A substituent on the aryl is the same or different, 1 to 3, preferably 1 to 2 substituent(s), including preferably halogen, hydroxy, amino, lower alkylamino, cyano, carboxy, formyl, oxo, lower alkyl, lower alkoxy, lower alkylthio, carbamoyl, and lower alkylcarbamoyl, and Substituent group S1(: optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substitutedphosphoric acid residue, hydroxyl substituted with optionally substitutedphosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue, lower alkyl substituted with optionally substituted phosphoric acid residue (said lower alkyl may be intervened with a hetero atom group(s) selected from O, S, SO, SO₂, NR⁵ (R⁵ is independently selected from the same substituent group for R⁴), —N═ and ═N—), lower alkoxy lower alkyl, amino lower alkyl optionally substituted with mono- or di-lower alkyl, halogenated lower alkyl, lower alkoxy, carbamoyl optionally substituted with mono- or di-lower alkyl, optionally substituted lower alkylsulfonylamino, halogenated lower alkoxy, hydroxyl lower alkyl), more preferably halogen, hydroxy,amino,cyano,lower alkyl, lower alkoxy or Substituent group S1, and most preferred is halogen (e.g., F) and/or a group selected from Substituent group S1. A substituent on the aryl is preferably at the 4-position, R² is more preferably phenyl or phenyl substituted with at least halogen, and most preferably 4-halogenophenyl (e.g., 4-F-phenyl). In another embodiment, R^(z) is preferably phenyl optionally substituted with 1 to 3 R(s) mentioned below.

In all compounds of the present invention, the structure of “—X—R²” is preferably shown by the formula below:

R each is independently a group selected from halogen and Substituent group S1.

Substituent group S1: optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxyl substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue, lower alkyl substituted with optionally substituted phosphoric acid residue (said lower alkyl may be intervened by a heteroatom group(s) selected from CO, O, S, SO, SO₂, NR^(a) (R^(a) is hydrogen or lower alkyl), —N═ and ═N—), lower alkoxy lower alkyl, optionally substituted amino lower alkyl (the substituent: mono- or di-lower alkyl, lower alkylcarbonyl, or lower alkylsulfonyl), halogenated lower alkyl, lower alkoxy, optionally substituted carbamoyl (the substituent; mono- or di-lower alkyl, lower alkylcarbonyl, or lower alkylsulfonyl), optionally substituted lower alkylsulfonylamino, halogenated lower alkoxy, and hydroxyl lower alkyl.

m is an integer of 0 to 3, preferably 0 or 1 to 2. when m is 1, R is preferably halogen. When m is 2, R is more preferably the same or different group selected from halogen, lower alkyl, lower alkoxy, lower alkoxylower alkyl, halogenated lower alkyl, halogenated lower alkoxy, lower alkylsulfonylamino, carbamoyl, and lower alkylcarbamoyl. More preferably, R is two halogens, or halogen and another group. R preferably locates at the 4-position and optional another position of the benzene ring.

R³ can be a various substituent which does not bring a negative effect to the pharmacological activity, including hydrogen, halogen, hydroxy, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycleoxy, and optionally substituted amino. Examples of substituent of “optionally substituted” include halogen, hydroxy, amino, lower alkylamino, cyano, carboxy, formyl, oxo, lower alkyl, lower alkoxy, lower alkylthio, carbamoyl, lower alkylcarbamoyl, aryl, heterocyclic group, lower alkylcarbonyl, lower alkylcarbonyloxy, lower alkoxycarbonyl, halogenated lower alkyl, halogenated lower alkoxy, and preferably halogen, hydroxy, amino, lower alkylamino, lower alkyl, and lower alkoxy. R³ is more preferably hydrogen, halogen, hydroxy, lower alkyl, lower alkenyl, lower alkoxy, lower alkenyloxy or optionally substituted amino, and most preferably hydrogen or lower alkyl (e.g., methyl), esp. hydrogen.

Z² shows C, CH, optionally substituted lower alkylene, lower alkenylene etc., and Z² and R⁴ of Z¹ taken together form a ring, whereby compound (I) shows a tricyclic compound (I-1) or (I-11) shown below, or its derivative, tetracyclic compound.

A ring is optionally substituted heterocycle containing at least an N atom. The heterocycle is a 5- to 7-membered ring which contains preferably 1 to 3, more preferably 2 to 3 atoms of O, S and/or N. The heterocycle is preferably selected from the above heterocycle, The arc optionally contains 1 to 2 heteroatom(s) at any possible position. One of preferable embodiments of A ring is an optionally substituted ring shown below.

(Z is CH₂, O, S, SO, SO₂ or NR¹⁹)

A ring is preferably a ring of (a), (b), or (c).

Z is preferably O or NR¹⁰.

When Z is NR¹⁹, examples of R¹⁹ include 1) hydrogen, 2) optionally substituted lower alkyl (the substituent is e.g., amino optionally substituted with mono- or di-lower alkyl; cycloalkyl; hydroxy; optionally substituted heterocyclic group (preferably 5- to 7-membered ring, e.g., furyl, thienyl, thiazolyl, pyridil, morpholino, imidazole; examples of the substituent include lower alkyl, halogen); optionally substituted heterocyclecarbonyl (the heterocycle is preferably 5- to 7-membered ring, e.g., morpholinocarbonyl); optionally substituted phenyl (the substituent is e.g., lower alkyl, amino, lower alkylamino, hydroxy, halogen, halogenated lower alkyl, lower alkoxy, halogenated lower alkoxy, lower alkylthio, lower alkylsulfonyl), acetylamino, carbamoyl, carbamoyl substituted with mono- or di-lower alkyl, lower alkylsulfonylamino, lower alkoxy, carbonyl, halogen, thiol, lower alkylthio), 3) lower alkenyl, 4) acyl (e.g., lower alkylcarbonyl), 5) lower alkylsulfonyl. 11¹⁰ may be selected from Substituent group S2 shown below,

The other substituent on A ring may be selected from R¹⁵ to R¹⁸ or Substituent group S2, preferably lower alkyl. Substituents on A ring may form a condensed ring or a Spiro ring as mentioned below, whereby compound (I) includes a tetracyclic compound.

A ring is more preferably any of the following rings:

(wherein, R²⁰ to R⁴⁰ are each independently a group selected from Substituent group S2, or any two groups of R²⁰ to R⁴⁰, which bonds to the same carbon atom, taken together with the carbon atom, may form a spiro ring, i.e., an optionally substituted carbocyle or optionally substituted heterocycle, or each combination of (R²⁰ and R²²), (R²³ and R²⁴), (R²⁵ and R²⁶), (R²⁷ and R²⁰), (R³⁰ and R³¹), (R³², and 13.³⁴), (R³⁵ and R³⁶), (R³⁷ and R³⁸), and (R³⁹ and R⁴⁰), taken together with the neighboring atom, may form an optionally substituted carbocyle or optionally substituted heterocycle.

Substitution group S2: hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocycle, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, hydroxy, optionally substituted amino, optionally substituted lower alkylcarbonyl, optionally substituted cycloalkylcarbonyl, optionally substituted cycloalkyl lower alkylcarbonyl, optionally substituted lower alkoxycarbonyl, optionally substituted arylcarbonyl, optionally substituted aryl lower alkylcarbonyl, optionally substituted aryl oxycarbonyl, optionally substituted heterocyclecarbonyl, optionally substituted heterocycle lower alkylcarbonyl, optionally substituted heterocycleoxycarbonyl, optionally substituted aminocarbonyl, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue, or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened with a heteroatom group(s) selected from CO, O, S, SO, SO₂, NR⁵ (R⁵ is independently selected from the same substitution group as R⁴), —N═ and ═N—)

The stereochemistry of an asymmetric carbon represented by * shows the R- or S-configuration, or a mixture thereof)

In one embodiment, R²⁰ to R⁴⁰ each is preferably hydrogen, optionally substituted lower alkyl (examples of the substituent: OH, lower alkoxy, cycloalkyl, lower alkylthio, lower alkylsulfonyl, heterocyclic group, aryl, optionally substituited amino (examples of the substituent: lower alkyl, acyl)), cycloalkyl, optionally substituited aryl (examples of the substituent: OH, lower alkyl), and optionally substituited heterocyclic group.

In one embodiment, R²⁰ to R²⁵, R²⁷ to R³⁰, and R³² to R³⁸, each is preferably hydrogen, C1-C8 alkyl, C6-C14 aryl C1-C8 alkyl, C6-C14 aryl, or alkoxy.

In one embodiment, R²⁶, R³¹, and R⁴⁰, each is preferably hydrogen, C3-6 cycloalkyl, heterocycle, or C1-8 alkyl optionally substituted with hydroxy, C3-6 cycloalkyl, alkoxy, heterocycle, heteroaryl, C6-14 aryl, or amino, wherein said amino may be optionally substituted with —C(O)C1-8 alkyl or C1-8 alkyl.

More Preferred embodiments are shown below for example

I) When A ring is A-1, preferred is that 1) Z is NR²⁵ and R²⁶ and R²⁴ taken together form heterocycle, and the others are hydrogens; 2) Z is O or NR²⁶, (R²⁰ and R³²) or (R²³ and R²⁴) taken together form cycloalkyl which is substituted with phenyl, the others are hydrogens or optionally substituted lower alkyl.

II) When A ring is A-2, preferred is that 1) Z is O, R²⁷ or R²⁸ is lower alkyl, and the others are hydrogens; 2) Z is NR³⁰ and R³⁰ and R³¹ taken together form heterocycle and the others are hydrogens, or R²⁷ and R²⁹ taken together form cycloalkyl and the others are hydrogens; 3) Z is O, R²⁷ and R²⁹ taken together form cycloalkyl which may be condensed with phenyl, and the others are hydrogens

R¹⁴ and R^(x) are each independently hydrogen, optionally substitutedlower optionally substituted cycloalkyl, optionally substituted cycloalkyl lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkoxy, optionally substituted lower alkenyloxy, optionally substituted aryl, optionally substituted aryl lower alkyl, optionally substituted aryloxy, optionally substituted heterocyclic group, optionally substituted heterocycle lower alkyl, optionally substituted heterocycleoxy, hydroxy, optionally substituted amino,optionally substituted lower alkylcarbonyl, optionally substituted cycloalkylcarbonyl, optionally substituted cycloalkyl lower alkylcarbonyl, optionally substituted lower alkoxycarbonyl, optionally substituted arylcarbonyl, optionally substituted aryl lower alkylcarbonyl, optionally substituted aryloxycarbonyl, optionally substituted heterocyclecarbonyl, optionally substituted heterocycle lower alkylcarbonyl, optionally substituted heterocycleoxycarbonyl, optionally substituted aminocarbonyl, optionally substituted phosphoric acid residue, aryl substituted with optionally substituted phosphoric acid residue, aralkyl substituted with optionally substituted phosphoric acid residue, hydroxy optionally substituted with optionally substituted phosphoric acid residue, amino substituted with optionally substituted phosphoric acid residue, or lower alkyl substituted with optionally substituted phosphoric acid residue (the lower alkyl may be intervened with a heterotom group(s) selected from O, S, SO, SO₂, NR^(a) (R^(a) is hydrogen or lower alkyl), —N═ and ═N—).

R¹⁴ and R^(x) are each independently, preferably, hydrogen, hydroxyl, optionally. substituted lower alkyl (the substituent is preferably, e.g., amino, lower alkyl amino, hydroxy, lower alkoxy). R¹⁴ and R^(x) are preferably hydrogens.

A broken line in the compound (I-1) represents the presence or absence of a bond, provided that when the broken line represents the presence of a bond, R^(X) is not present.

The compound (I) includes the following compounds,

(wherein each symbol is as defined above)

F ring means the same heterocycle as A ring, preferably 5to 7-membered ring, and the substituents on F ring are the same as those for A ring. The other symbols are as defined above.

(wherein each symbol is as defined above; Z is O or NR¹⁰; R¹⁵ to R¹⁹ are each independently hydrogen or a group selected from the above Substituent group S2, or each combination of (R¹⁵ and R¹⁶), (R¹⁷ and R¹⁸), (R¹⁰ and R¹⁸), and (R¹⁸ and R¹⁹) taken together with the neighboring atom(s), may form an optionally substituted carbocycle (preferably 5- to 6-membered ring) or an optionally substituted heterocyle (preferably 5- to 6-membered ring); or each combination of (R¹⁵ and R¹⁹) and (R¹⁷ and R¹⁸) taken together may fom oxo)

Compound (I-3) is preferably as follows.

-   (1) R¹ is hydrogen; R^(a) is hydrogen; m is 1 or 2; R¹⁴ is hydrogen. -   (2) m is 1 or 2, R is each independently halogen, halogenated lower     alkyl, lower alkoxy, halogenated lower alkoxy, lower alkoxy lower     alkyl, hydroxy lower alkyl, optionally substituted amino lower alkyl     (the substituent is mono- or di-lower alkyl, lower alkylcarbonyl, or     lower alkylsulfonyl), optionally substituted carbamoyl (the     substituent is mono or di-lower alkyl, lower alkylcarbonyl, or lower     alkylsulfonyl), phosphoric acid residue, aryl substititted with     optionally substituted phosphoric acid residue, aralkyl substituted     with optionally substituted phosphoric acid residue or sulfonylamino     optionally substituted with lower alkyl; R¹ is hydrogen; R³ is     hydrogen; R¹⁴ is hydrogen, hydroxyl or lower alkyl optionally     substituted with mono- or di-lower alkylamino; Z is O or NR¹⁹ (R¹⁹     is hydrogen or lower alkyl, lower alkoxy lower alkyl, optionally     substituted phosphoric acid residue, aryl substituted with     optionally substituted phosphoric acid residue, aralkyl substituted     with optionally substituted phosphoric acid residue, hydroxy     substituted with optionally substituted phosphoric acid residue,     amino substituted with optionally substituted phosphoric acid     residue, or lower alkyl substituted with optionally substituted     phosphoric acid residue), -   (3) R is each independently, —F, —CF₃, —OMe, —OCF₃, —CH₂OMe, —CH₂OH,     —CH₂N(Me)₂, —CONHMe, —CON(Me)₂, —CH₂PO(OEt)₂, —PO(OEt)₂, —NHSO₂Me,     or —NMeSO₂Me; R¹ is hydrogen; R³ is hydrogen; m is 1 or 2; R¹⁴ is     hydrogen, hydroxyl or —CH₂N(Me)₂; Z is O or NR¹⁹ (R¹⁹ is hydrogen or     —CH(Me)₂, —(CH₂)₂OMe, —(CH₂)₂PO(OEt)₂). -   (4) R¹⁵ and R¹⁶ are hydrogens; R¹⁷ and R¹⁸ are hydrogens or taken     together with the neighboring atom forma 3- to 7-membered carbocyle;     and/or Z is O or NH. This case preferably also satisfys the     above (2) or (3).

D ring means the same heterocycle as A ring, preferably 5- to 7-membered ring, and the substituents on D ring are the same as those for A ring. The other symbols are as defined above.

The structure of compound (I) has at least the following characteristics,

-   (1) The main structure, condensed heterocycle, is substituted with     oxo (═O), hydroxyl (OH) and oxo, -   (2) A substituted carbamoyl group (—CONR¹XR²) is attached to the     position neighboring to the oxo group on the condensed hereocycle.

The above structure contributes to a remarkably potent integrase inhibitory activity and/or cell-growth inhibitory activity against virus including HIV. In contrast, the structures of the other parts such as Z¹, Z², and Z³ each may be of variety, being optionally substituted or optionally condensed, and its condensed ring is also optionally substituted.

The present invention provides a pharmaceutically acceptable salt or a solvate of compound (I). All theoretically possible tautomer, geometrical isomer, optically active compound, and racemate thereof are within the scope of the invention.

Pharmaceutically acceptable salts of a compound of the present invention include, as basic salts, for example, alkali metal salts such as sodium or potassium salts; alkaline-earth metal salts such as calcium or magnesium salts; ammonium salts; aliphatic amine salts such as trimethylamine, triethylamine, dicyclohexylamine, ethanolamine, diethanolamine, triethanolamine or procaine salts; aralkyl amine salts such as N,N-dibenzylethylenecliainine salts; heterocyclic aromatic amine salts such as pyridin salts, picoline salts, quinoline salts or isoquinoline salts; quaternary ammonium salts such as tetrarnethylammonium salts, tetraethylammonium salts, benzyltrimethylammonium salts, benzyltriethylammonium salts, benzyltributylammonium salts, methyltrioctyla mmonium salts or tetrabutylammonium salts; and basic amino acid salts such as arginine salts or lysine salts, Acid salts include, for example, mineral acid salts such as hydrochloride, sulfates salts, nitrate salts, phosphates salts, carbonates salts, hydrogencarbonatcs or perchlorate; organic acid salts such as acetates, propionates, lactates, maleates, fumarates, tararic acid salts, malates, citrates salts, ascorbates, formic acid; sulfonates such as methanesulfonates, isethionates, benzenesulfonates, or p-toluenesulfonates; and acidic amino acid salts such. as aspartates or glutamates.

Solvates of a compound of the present invention include alcholates and hydrates.

A general process for producing the present compound will be exemplified below.

(Method of Preparing Raw Material)

(wherein L¹ is a leaving group (e.g.: halogen); P¹ and P² are a hydroxy protecting group; P⁰ is a carboxy protecting group (e.g.: lower alkyl); R^(a) and R^(b) are hydrogen or a substituent on an amino group)

Examples of a hydroxy protecting group (P¹, P²) include acyl (e.g.: acetyl, pivaloyl, benzoyl), aralkyl (e.g.: benzyl), lower alkyl (e.g.: methyl), alkoxyalkyl (e.g.: methoxymethyl, methoxyethyl), lower alkylsulfonyl (e.g.: methane sulfonyl), arylsulfonyl (e.g.: benzenesulfonyl, toluenesulfonyl), alkoxycarbonyl methoxycarbonyl) and the like.

As a carboxy protecting group (P³), lower alkyl (e.g.: methyl, ethyl), and aralkyl benzyl) are exemplified.

(First Step)

The present step is a reaction for condensing a compound (II) and a compound (III) to synthesize a compound (IV). The reaction may be performed according to the condition for a reaction of amidating carboxylic acid which is generally performed. A compound (II) may be reacted as it is, or may be reacted after converted into corresponding acid chloride or active ester. Preferably, the reaction is performed in a suitable solvent in the presence of a condensing agent.

As a condensing agent, dicyclohexylcarbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and the like may be used. If necessary, a reagent such as 1-hydroxybenzotriazole and N-hydroxysuccinimide, or a base such as triethylamine, N-methylmorpholine, and pyridine may be added.

A reaction temperature is 0 to 150° C., preferably room temperature to 70° C.

As a reaction solvent, a non-protonic solvent can be broadly used, and tetrahydrofuran (THF), 1,4-dioxane, dimethylformamide (DMF), methylene chloride, chloroform and the like are preferable.

A reaction time is a few minutes to a few tens hours, preferably 9 to 17 hours.

(Second Step)

The present step is a reaction for introducing a protected hydroxy group (OP¹) into a compound (IV) to produce a compound (V). The reaction may be performed according to the condition for an alkoxylating reaction which is generally performed.

For example, a compound (V) in which P¹ is methyl can be synthesized by reacting a compound (IV) with metal alkoxide (e.g.: sodium methoxide).

A reaction temperature is 0 to 200° C., preferably 80 to 120° C.

As a reaction solvent, alcohol, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO) are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 5 to 10 hours.

(Third Step)

The present step is a reaction for protecting a hydroxy group of a compound (V) to produce a compound (VI). The reaction may be performed according to the condition for a reaction of protecting a hydroxy group which is generally performed. For example, by using diisopropyl azodicarboxylate or diethyl azodicarboxylate together with an alcohol and various phosphines, a compound (VI) in which P² is alkyl can be synthesized.

A reaction temperature is 0 to 100° C., preferably 0° C. to room temperature.

As a reaction solvent, THF, toluene, dichloromethane and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 3 hours.

(Fourth Step)

The present step is a reaction of oxidizing a nitrogen atom of a compoung (VI) to produce a compound (VII). The reaction may be performed according to the condition for an oxidation reaction using an oxidizing agent which is generally performed.

A reaction temperature is 0 to 100° C., preferably under ice cooling to room temperature.

As a reaction solvent, chloroform, methylene chloride, acetic acid and the like are exemplified.

Examples of an oxidizing agent include metachloroperbenzoic acid, hydrogen peroxide and the like.

A reaction time is a few minutes to a few tens hours, preferably 1 to 5 hours.

(Fifth Step)

The present step is a reaction for hydroxylating a methyl group of a compound (VII). Preferably, after acetoxylation by a reaction with acetic anhydride (reaction temperature: 0 to 150° C., preferably 120 to 140° C.), this may be hydrolyzed (e.g.: treatment with a base (e.g.: alkali metal hydroxide)).

A reaction time is a few minutes to a few tens hours, preferably 0.5 to 2 hours for acetoxylation, and 0.5 to 1 hour for hydrolysis.

(Sixth Step)

The present step is a reaction for oxidizing a hydroxy group of a compound (VIII) to synthesize a compound (IX).

A reaction temperature is 0 to 150° C., preferably room temperature to 70° C.

As a reaction solvent, chloroform and the like are exemplified.

As an oxidizing agent, dimethyl sulfoxide and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 0.1 to 1 hour.

(Seventh Step)

The present step is a reaction for oxidizing a formyl group of a compound (IX) to synthesize a compound (X).

A reaction temperature is 0 to 150° C., preferably under ice-cooling to room temperature.

As a reaction solvent, an alcohol and the like are exemplified.

As an oxidizing agent, potassium hydroxide and iodine are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 0.5 to 3 hours.

(Eighth Step)

The present step is a reaction for deprotecting an OP² part of a compound (X) to synthesize a compound (XI). The reaction may be performed according to the condition for a reaction of deprotecting a hydroxy protecting group which is generally performed.

A reaction temperature is 0 to 150° C., preferably under ice-cooling to room temperature.

As a reaction solvent, acetonitrile, methylene chloride, THF and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 3 hours.

(Ninth Step)

The present step is a reaction for deprotecting an OP¹ part of a compound (XI) to synthesize a compound (I-A). The reaction may be treated preferably with a Lewis acid (e.g.: aluminum chloride).

A reaction temperature is 0 to 150° C., preferably 10 to 0° C.

As a reaction solvent, methylene chloride, THF and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 3 hours.

(Tenth Step)

The present step is a reaction for deprotecting an ester part (COOP⁸) of a compound (X) to synthesize carboxylic acid (XII). Preferably, hydrolysis with an alkali (e.g.: NaOH) may be performed.

A reaction temperature is 0 to 150° C., preferably 10 to 50° C.

As a reaction solvent, methanol, water and the like are exemplified,

A reaction time is a few minutes to a few tens hours, preferably a few minutes to 2 hours.

Carboxylic acid (XII) can be converted into various derivatives (e.g.: amide).

(Eleventh Step)

The present step is a reaction for reacting a compound (XII) with various amines to synthesize a compound (XIII). The reaction may be performed according to the condition for a reaction of amidating carboxylic acid which is generally performed and, for example, the reaction may be performed as in the first step.

A reaction temperature is 0 to 150° C., preferably room temperature to 70° C.

As a reaction solvent, a non-protonic solvent can be broadly used, and tetrahydrofuran (THF), 1,4-dioxane, dimethylformamide (DMF), methylene chloride, chloroform and the like are preferable.

A reaction time is a few minutes to a few tens hours, preferably a few minutes to 3 hours.

An amide part of the resulting compound (XIII) may be further chemically modified (e.g.: N-alkylation).

(Twelfth Step)

The present step is a reaction for deprotecting OP¹ and OP² parts of a compound (XIII) to synthesize a compound (I-B). The reaction may be performed according to the condition for a reaction of deprotecting a hydroxy protecting group which is generally performed.

For example, when pyridine hydrochloride is used, a reaction temperature is 0 to 200° C., preferably 150 to 180 degree.

A reaction time is a few minutes to a few tens hours, preferably 1 to 5 minutes.

(Thirteenth Step)

The present step is a reaction for deprotecting an ester part (COOP⁸) of a compound (XI) to synthesize carboxylic acid (XIV). Preferably, hydrolysis with an alkali (e.g.,: lithium hydroxide) may be performed.

A reaction temperature is 0 to 150° C., preferably 10 to 50° C.,

As a reaction solvent, methanol, water and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably a few minutes to 3 hours.

(Fourteenth Step)

The present step is a reaction for deprotecting an OP¹ part of a compound (XIV) to synthesize a compound (I-C). The reaction may be treated preferably with a Lewis acid (e.g.: boron tribromide).

A reaction temperature is 0 to 150° C., preferably under ice-cooling to room temperature.

As a reaction solvent, dichloromethane and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably a few minutes to 5 hours.

The monocyclic carbamoylpyridone derivative obtained above is derived into a bicyclic compound by the following method.

(Process 1)

(wherein R¹, X, R², P¹, P³ and R⁴ are as define above, and L² is a leaving group such as halogen etc.)

(Fifteenth Step)

The present step is a reaction for reacting the compound (XI) or a compound (XI′) which is a tautomer thereof with an allyl compound to synthesize a compound (XV). A compound (XI′) can be synthesized, for example, according to the method of Example A-1.

The reaction is performed preferably in the presence of a base (e.g.: cesium carbonate).

A reaction temperature is 0 to 100° C., preferably 10 to 40° C.

As a reaction solvent, diinethylformamide and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 10 hours.

(Sixteenth Step)

The present step is a reaction for oxidizing a compound (XV) to synthesize a compound (XVI). As an oxidizing agent, osmium tetraoxide and alkali metal osmium tetraoxide (e.g.: K₂OsO₄) are exemplified.

A reaction temperaturwe is 0 to 100° C., preferably 10 to 40° C.

As a reaction solvent, 1,4-dioxane, tetrahydrofuran and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 5 hours.

(Seventeenth Step)

The present step is a reaction for reacting a compound (XVI) with amine (XVII) to perform dehydration condensation to synthesize a compound (XVIII).

A reaction temperature is 0 to 200° C., preferably 140 to 180° C.,

As a reaction solvent, methylene chloride, acetonitrile and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 0.5 to 1.5 hours,

(Eighteenth Step)

The present step is a reaction for deprotecting a compound (XVIII) preferably with an acid to synthesize a compound (XIX), and may be performed according to the condition for a conventional reaction of deprotecting a protected hydroxy group.

A reaction temperature is 0 to 200° C.

As an acid, pyridine hydrochloride, trifluoroacetic acid and the like are exemplified.

As a reaction solvent, the acid and trimethylsilyl iodide are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 15 minutes to 1 hour.

(Nineteenth Step)

The present step is a reaction for reducing a compound (XVIII) to synthesize a compound (XX).

As a reducing agent, H₂/Pd—C and the like are exemplified.

A reaction temperatuer is 0 to 100° C., preferably 10 to 30° C.

As a reaction sovelnt, dimethylformamide, methanol, tetrahydrofuran and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 5 to 20 hours.

(Process 2)

The intermediate (XVIII) may be also synthesized by a method shown below.

(Twentieth Step)

The present step is a reaction for reacting a compound (XIV) with a compound (XXI) to synthesize a compound (XXII). The present reaction may be performed according to the condition for a conventional amidation reaction.

A reaction temperature is 0 to 100° C., preferably 0 to 50° C.

As a reaction solvent, dimethylformamide, methylene chloride, tetrahydrofuran and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 10 hours.

(Twenty-First Step)

The present step is a reaction for reacting a compound (XXII) with an acid to perform deprotection and intramolecular ring closure, to synthesize a compound (XXIII). The present reaction may be performed according to the condition for a conventional reaction of deprotecting acetal.

A reaction temperature is 0 to 100° C., preferably room temperature to 80° C.

As a reaction solvent, dioxane, tetrahydrofuran and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 0.5 to 1 hour.

As an acid, hydrochloric acid, and paratoluenesulfonic acid are exemplified.

(Twenty-Second Step)

The present step is a reaction for dehydrating a compound (XXIII) to synthesize a compound (XXIV). The present reaction may be peformed according to the condition for a conventional dehydration reaction.

A reaction temperature is 0 to 100° C., preferably room temperature to 80° C.

As a reaction solvent, acetonitrile, methylene chloride and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 5 hours,

(Process 3)

(Twenty-Third Step)

The present step is a reaction for reacting a compound (XVI) with amine (XXIV) to perform dehydration condensation to synthesize a compound (XXV) according to the seventeenth step or a method of synthesizing a compound 17-1, Preferably, as a reaction catalyst, an acid (e.g.: acetic acid) is added, and a microwave reaction apparatus is used.

A reaction temperature is 0 to 200° C., preferably 140 to 180° C.

As a reaction solvent, methylene chloride, acetonitrile and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 0.5 to 1.5 hours.

(Twenty-Fourth Step)

The present step is a reaction for deprotecting a compound (XXV) preferably with an acid to synthesize a compound (XXVI) according to the eighteenth step, and may be performed according to the condition for a conventional reaction of deprotecting a protected hydroxy group.

A reaction temprature is 0 to 200° C.

As an acid, pyridine hydrochloride, trifluoroacetic acid and the like are exemplified.

As a reaction solvent, the aforementioned acid and trirnethylsilyl iodide are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 15 minutes to 1 hour.

(Process 4)

(Twenty-Fifth Step)

The present step is a reaction for reacting a compound (XIV) with a compound (XXIV) to synthesize a compound (XXVII) according to the twentieth step, The present reaction may be performed according to the condition for a conventional amidation reaction.

A reaction teinperature is 0 to 100° C., preferably 0 to 50° C.

As a reaction solvent, dimethylformamide, methylene chloride, tetrahydrofuran and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 10 hours.

(Twenty-Sixth Step)

The present step is a reaction for reacting a compound (XXVII) or a tautomer thereof with an allyl compound to synthesize a compound (XXVIII) according to the fifteenth step.

A reaction is performed preferably in the presence of a base (e.g.: cesium carbonate).

A reaction temperature is 0 to 100° C., preferably 10 to 40° C.,

As a reaction solvent, dimethylformamide and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 10 hours.

(Twenty-Seventh Step)

The present step is a reaction for oxidizing a compound (XXVIII) to synthesize a compound (XXIX) according to the sixteenth step,

As an oxidizing agent, osmium tetraoxide and alkali metal osmium tetraoxide (e.g.: K₂OsO₄) are exemplified.

A reaction temperature is 0 to 100° C., preferably 10 to 40° C.

As a reaction solvent 1,4-dioxane, tetrahydrofuran and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 1 to 5 hours.

(Twenty-Eighth Step)

The present step is a reaction for dehydration-condensing a compound (XXIX) to synthesize a compound (XXX) according to the seventeenth step or a method of synthesizing a compound 17-1. Preferably, as a reaction catalyst, an acid (e.g.: acetic acid) is added, and a microwave reaction' apparatus is used.

A reaction temperature is 0 to 200° C., preferably 140 to 180° C.

As a reaction solvent, methylene chloride, acetonitrile and the like are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 0.5 to 1.5 hours.

(Twenty-Ninth Step)

The present step is a reaction for deprotecting a compound (XXX) preferably with an acid to synthesize a compound (XXXI) according to the eighteenth step, and may be performed according to the condition for a conventional reaction of deprotecting a protected hydroxy group.

A reaction temperature is 0 to 200° C.

As an acid, pyridine hydrochloride, trifluoroacetic acid and the like are exemplified.

As a reaction solvent, the aforementioned acid and trimethylsilyl iodide are exemplified.

A reaction time is a few minutes to a few tens hours, preferably 15 minutes to 1 hour,

(Process 5)

A compound (I-3) in which Z is NR¹⁹ can be synthesized according to the following reaction scheme, according to Process 4.

(Process 10)

(wherein respective symbols are as defined above)

(Forty-Ninth Step)

A compound (XIV-16) is obtained by reacting a compound (XIV) with an amine reagent, according to the thirty-fifth step.

(Fiftieth Step)

A compound (XIV-17) is obtained by subjecting a compound (XIV-16) to a general acetal deprotecting reaction according to the forty-fourth step.

(Fifty-First Step)

A compound (XIV-18) is obtained CD ring formation) by deprotecting a P¹ part of a compound (XIV-14) according to the thirty-eighth step.

The present invention further provides various intermediates (I-P) shown below and a process for preparing the same, as well as a process for preparing the above mentioned compound (I) comprising the deprotection of the intermediate.

(Intermediates)

(P¹ is a hydroxyl-protecting group; the other symbols are as defined above)

Preferred compounds are shown below. Each P¹ is a hydroxyl-protecting group, such as C₆₋₁₄arylC₁₋₈alkyl (e.g., benzyl (=Bn)).

Preferably, wherein R^(e) is one or two halogen; R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; R^(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; R^(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(a) is one or two halogen; R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is one or two halogen; R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

Preferably, wherein R^(e) is halogen; and P¹ is C₆₋₁₄arylC₁₋₈alkyl;

The above intermediates, compound (I-20a), (I-20b), (I-21a), (I-21b), (I-22a), (I-22b), (I-23a), (I-23b), (I-24a), (I-24b), (I-25), (I-26), or (I-27), can be prepared by condensing a compound of the formula:

-   -   wherein R^(e) is one or two halogen; and R⁵⁰ is C₁₋₈alkyl;

-   with each amine shown below, respectively:

wherein R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy;

wherein R^(z) is C₁₋₈alkyl, C₆₋₁₄arylC₁₋₈alkyl, C₆₋₁₄aryl, or alkoxy;

wherein R^(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₅alkyl;

wherein R^(z) is C₁₋₈alkyl; R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

wherein R^(z1) is hydrogen, C₃₋₆cycloalkyl heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

wherein R^(z1) is hydrogen, C₃₋₆cycloalkyl, heterocycle, or C₁₋₈alkyl optionally substituted with hydroxy, C₃₋₆cycloalkyl, alkoxy, heterocycle, heteroaryl, C₆₋₁₄aryl, or amino, wherein said amino may be optionally substituted with —C(O)C₁₋₈alkyl or C₁₋₈alkyl;

The condition for the above condensation is illustrated below for example.

Examples of the solvent include halocarbons such as dichloromethane, dichloroethane, and acetic acid.

The reaction temperature is preferably, 0 to 200° C., more preferably, 50 to 170° C.

The reaction time is usually several minutes to several hours.

The above intermediates, compound (I-20a), (I-20b), (I-21a), (I-21b), (I-22a), (I-22b), (I-23a), (I-23b), (I-24a), (I-24b), (I-26), or (I-27), can be deprotected to give each corresponding deprotected compound wherein P¹ is hydrogen, or its pharmaceutically acceptable salt, which are encompassed within the scope of compound (I) of the present invention,

In addition, the present compound obtained above may be further chemically modified to synthesize another compound. In addition, when there is a reactive functional group (e.g.: OH, CO OH, NH₂) on a side chain part etc. in the above reaction, the group may be protected before the reaction and may be deprotected after the reaction, if desired.

The present compound is useful, for example, as a drug such as an anti-virus drug. The present compound has the remarkable inhibitory action on integrase of a virus. Therefore, the present compound can be expected to have the preventive or therapeutic effect for various diseases derived from a virus which produces at least integrase, and is grown at infection in an animal cell, and is useful as an integrase inhibiting agent for retrovirus (e.g. HIV-1, HIV-2, HTLV-1, SIV, FIV etc.), and is useful as an anti-HIV drug etc.

In addition, the present compound may he used in joint use therapy by combining an anti-HIV drug having the different action methanism such as a reverse trascriptase inhibiter and/or a protease inhibiting agent. Particularly, currently, an integrase inhibitor is not marketed, and it is useful to use in joint use therapy by combining the present compound with a reverse transcriptase inhibiter and/or a protease inhibiter.

Further, the above use includes not only use as a medical mixture for anti-HIV, but also use as a joint use agent for increasing the anti-HIV activity of other anti-HIV drug such as cocktail therapy.

In addition, the present compound can be used in order to prevent infection with a retrovirus vector from spreading into a tissue other than an objective tissue, upon use of a retrovirus vector based on HIV or MLV in the field of gene therapy. Particularly, when a cell is infected with a vector in vitro, and the cell is returned into a body, if the present compound is administered in advance, extra infection can be prevented in a body.

The present compound can be adminitetered orally or parenterally. In the case of oral administration, the present compound can be also used as a conventional preparation, for example, as any dosage form of a solid agent such as tablets, powders, granules, capsules and the like; an aqueous agent; an oily suspension; or a liquid agent such as syrup and elixir. In the case of parenteral administration, the present compound can be used as an aqueous or oily suspension injectable, or a nasal drop. Upon preparation of it, conventional excipients, binders, lubricants, aqueous solvents, oily solvents, emulsifiers, suspending agents, preservatives, stabilizers and the like may be arbitrarily used. As an anti-HIV-drug, particularly, an oral agent is preferable. A preparation of the present invention is prepared by combining (e.g. mixing) a therapeutically effective amount of the present compound with a pharmaceutically acceptable carrier or diluent.

A dose of the present invention is different depending on an administration method, an age, a weight and condition of a patient, and a kind of a disease and, usually, in the case of oral administration, about 0.05 mg to 3000 mg, preferably about 0.1 mg to 1000 mg may be administered per adult a day, if necessary, by dividing the close. In addition, in the case of parenteral administration, about 0.01 mg to 1000 mg, preferably about 0.06 mg to 600 mg is administered per adult a day.

Examples are shown below.

EXAMPLE A-1 9-Hydroxy-2-(2-methoxy-ethyl)-dioxo-1,8-dihydro-2H-pyrid[1,2-a]pyrazine-7-carboxylic acid 4-fluorobanzylamide EXAMPLE B-1 9-Hydroxy-2-(2-methoxy-ethyl)-dioxo-1,8-tetrahydro-2H-pyrid[1,2-a]pyrazine-7-carboxylic acid 4-fluoro-benzylamide

-   1) Mantol 1 (189 g, 1.5 mol) was dissolved in dimethylformamide     (1890 ml)), and benzyl bromide (184 ml, 1.5 mol) was added. After     the solution was stirred at 80° C. for 15 minutes, potassium     carbonate (228 g, 1.65 mol) was added, and the mixture was stirred     for 1 hour. After the reaction solution was cooled to room     temperature, an inorganic salt was filtered, and the filtrate was     distilled off under reduced pressure. To the again precipitated     inorganic salt was added tetrahydrofuran (1000 ml)), this was     filtered, and the filtrate was distilled off under reduced pressure     to obtain the crude product (329 g, >100%) of     3-benzyloxy-2-methyl-pyran-4-one 2 as a brown oil. NMR (CDCl₃) δ:     2.09(3H, s), 5.15(2H, s), 6.36(1H, d, J=5.61 Hz), 7.29-7.41(5H, m),     7.60(1H, d, J=5.6 Hz). -   2) The compound 2 (162.2 g, 750 mmol) was dissolved in ethanol (487     ml), and aqueous ammonia (28%, 974 ml)) and a 6N aqueous sodium     hydroxide solution (150 ml, 900 mmol) were added. After the reaction     solution was stirred at 90° C. for 1 hour, this was cooled to under     ice cooling, and ammonium chloride (58 g, 1080 mmol) was added. To     the reaction solution was added chloroform, this was extracted, and     the organic layer was washed with an aqueous saturated sodium     bicarbonate solution, and dried with anhydrous sodium sulfate. The     solvent was distilled off under reduced pressure, isopropyl alcohol     and diethyl ether were added to the residue, and precipitated     crystals were filtered to obtain     3-benzyloxy-2-methyl-1H-pyridine-4-one 3 (69.1 g, 43%) as a pale     yellow crystal.

NMR (DMSO-d₆) δ: 2.05(3H, s), 5.04(2H, s), 6.14(1H, d, J=7.0 Hz), 7.31-7.42(5H, m), 7.46(1H, d, J=7.2 Hz), 11.29(1H, brs).

-   3) The above compound 3 (129 g, 599 mmol) was suspended in     acetonitrile (1300 ml)), and N-bromosuccinie acid imide (117 g, 659     mmol) was added, followed by stirring at room temperature for 90     minutes. Precipitated crystals were filtered, and washed with     acetonitrile and diethyl ether to obtain     3-benzyloxy-5-bromo-2-methyl-pyridine-4-ol 4(154 g, 88%) as a     colorless crystal. NMR (DMSO-d₆) δ: 2.06(3H, s), 5.04(2H, s),     7.32-7.42(5H, m), 8.03(1H, d, J=5.5 Hz), 11.82(1H, brs). -   4) To a solution of the compound 4 (88 g, 300 mmol), palladium     acetate (13.4 g, 60 mmol) and 1,3-bis(diphenylphosphino)propane     (30.8 g, 516 mmol) in dimethylformamide (660 ml)) were added     methanol (264 ml)) and triethylamine (210 ml, 1.5 mol) at room     temperature. The interior of a reaction vessel was replaced with     carbon monoxide, and the material was stirred at room temperature     for 30 minutes, and stirred at 80 degree for 18 hours. A vessel to     which ethyl acetate (1500 ml)), an aqueous saturated ammonium     chloride solution (1500 ml)) and water (1500 ml)) had been added was     stirred under ice-cooling, and the reaction solution was added     thereto. Precipitates were filtered, and washed with water (300     ml)), ethyl acetate (300 ml)) and diethyl ether (300 ml)) to obtain     5-benzyloxy-4-hydroxy-6-methyl-nicotinic acid methyl ester 5 (44.9     g, 55%) as a colorless crystal.

NMR (DMSO-d₆)δ: 2.06(3H, s), 3.72(3H, s), 5.02(2H, s), 7.33-7.42(5H, m), 8.07(1H, s).

-   5) After a solution of the compound 5 (19.1 g, 70 mmol) in acetic     anhydride (134 ml)) was stirred at 130° C. for 40 minutes, the     solvent was distilled off under reduced pressure to obtain     4-acetoxy-5-benzyloxy-6-methyl-nicotinic acid methyl ester 6 (19.9     g, 90%) as a flesh colored crystal.

NMR (CDCl₃)δ: 2.29(3H, s), 2.52(3H, s), 3.89(3H, s), 4.98(2H, s), 7.36-7.41(5H, m), 8.85(1H, s).

-   6) To a solution of the compound 6 (46.2 g, 147 mmol) in chloroform     (370 ml)) was added metachloroperbenzoic acid (65%) (42.8 g, 161     mmol) in portions under ice-cooling, and this was stirred at room     temperature for 90 minutes. To the reaction solution was added a 10%     aqueous potassium carbonate solution, and this was stirred for 10     minutes, followed by extraction with chloroform. The organic layer     was washed with successively with a 10% aqueous potassium carbonate     solution, an aqueous saturated ammonium chloride solution, and an     aqueous saturated sodium chloride solution, and dried with anhydrous     sodium sulfate. The solvent was distilled off under reduced     pressure, and the residue was washed with diisopropyl ether to     obtain 4-acetoxy-5-benzyloxy-6-methyl-1-oxynicotinic acid methyl     ester 7 (42.6 g, 87%) as a colorless crystal.

NMR (CDCl₃)δ: 2.30(3H, s), 2.41(3H, s), 3.90(3H, s), 5.02(2H, s), 7.37-7.39(5H, m), 8.70(1H, s).

-   7) To acetic anhydride (500 ml)) which had been heated to stir at     130° C. was added the compound 7 (42.6 g, 129 mmol) over 2 minutes,     and this was stirred for 20 minutes. The solvent was distilled off     under reduced pressure to obtain     4-acetoxy-6-acetoxymethyl-5-benzyloxy-nicotinic acid methyl ester 8     (49.6 g, >100%) as a black oil.

NMR (CDCl₃)δ: 2.10(3H, s), 2.28(3H, s), 3.91(3H, s), 5.07(2H, s), 5.20(2H, s), 7.35-7.41(5H, m), 8.94(1H, s).

-   8) To a solution of the compound 8 (46.8 g, 1.25 mmol) in methanol     (140 ml)) was added a 2N aqueous sodium hydroxide solution (376 ml))     under ice-cooling, and this was stirred at 50° C. for 40 minutes. To     the reaction solution were added diethyl ether and 2N hydrochloric     acid under ice-cooling, and precipitated crystals were filtered.     Resulting crystals were washed with water and diethyl ether to     obtain 5-benzyloxy-4-hydroxy-6-hydroxymethyl-nicotinic acid 9 (23.3     g, 68%) as a colorless crystal.

NMR (DMSO-d₆)δ: 4.49(2H, s), 5.19(2H, s), 5.85(1H, brs), 7.14-7.20(2H, m), 7.33-7.43(7H, m), 8.30(1H, s), 10.73(1H, t, J=5.8 Hz), 11.96(1H, brs).

-   9) To a solution of the compound 9 (131 g, 475 mmol),     1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (219 g,     1140 mmol) and 1-hydroxybenzotriazole (128 g, 950 mmol) in     dimethylformamide (1300 ml)) was added 4-fluorobenzylamine (109 ml,     950 mmol), and this was stirred at 80° C. for 1.5 hours. After the     reaction solution was cooled to room temperature, hydrochloric acid     was added, followed by extraction with ethyl acetate. The extract     was washed with a 5% aqueous potassium carbonate solution, an     aqueous saturated ammonium chloride solution, and an aqueous     saturated sodium chloride solution, and dried with anhydrous sodium     sulfate. The solvent was distilled off under reduced pressure to     obtain a mixture (175 g) of 10 and 11, the resulting mixture was     dissolved in acetic acid (1050 ml)) and water (1050 ml)), and zinc     (31.1 g, 475 mmol) was added, followed by heating to reflux for 1     hour. After the reaction solution was cooled to room temperature, a     10% aqueous potassium carbonate solution was added, followed by     extraction with ethyl acetate. The extract was washed with an     aqueous saturated ammonium chloride solution, and an aqueous     saturated sodium chloride solution, and dried with anhydrous sodium     sulfate. After the solvent was distilled off under reduced pressure,     this was washed with diethyl ether to obtain     5-benzyloxy-N-(4-fluoro-benzyl)-4-hydroxy-6-hydroxymethyl-nicotinic     acid amide 10 (107 g, 59%) as a colorless crystal.

NMR (DMSO-d₆)δ: 4.45(2H, d, J=4.3 Hz), 4.52(2H, d, J=5.8 Hz), 5.09(2H, s), 6.01(1H, brs), 7.36-7.43(5H, m), 8.31(1H, s), 12.63(1H, brs).

-   10) After manganese dioxide (49 g) was added to a suspension of the     compound 10 (9.8 g, 25.6 mmol) in chloroform (490 ml)), the mixture     was stirred at room temperature for 1 hour. After the reaction     solution was stirred at 60° C. for 20 minutes, Celite filtration was     performed, and this was washed with chloroform heated at 50° C. The     filtrate was distilled off under reduced pressure to obtain     5-benzyloxy-N-(4-fluoro-benzyl)-6-formyl-4-hydroxy-nicotinic acid     amide 12 (8.2 g, 84%) as a pale yellow crystal.

NMR (DMSO-d₆)δ: 4.53(2H, d, J=5.8 Hz), 5.38 (2H, s), 7.15-7.21(2H, m), 7.35-7.46(7H, m), 8.33(1H, s), 0.90(1H, s), 10.35(1H, t, J=5.8 Hz), 12.49(1H, brs).

-   11) To an aqueous solution (105 ml)) of sodium chlorite (7.13 g,     78.8 mmol), and sulfamic acid (7.65 g, 78.8 mmol) was added a     solution of the compound 12 (15.0 g, 39.4 mmol) in tetrahydrofuran     (630 ml)) under ice-cooling, and the mixture was stirred at room     temperature for 1 hour. After water (2500 ml) was added to the     reaction solution, precipitated crystals were filtered. Washing with     diethyl ether afforded     3-benzyloxy-5-(4-fluoro-benzylcarbamoyl)-4-hydroxy-pyridine-2-carboxylic     acid 13 (14.0 g, 90%) as a colorless crystal.

NMR (DMSO-d₆)δ: 4.52(2H, d, J=5.81 Hz), 5.13 (2H, s), 7.14-7.19(2H, m), 7.31-7.40(5H, m), 7.47-7.49(2H, m), 8.31(1H, d, J=4.5 Hz), 10.44(1H, t, J=5.9 Hz), 12.47(1H, brs).

12) A solution of the compound 13 (198 mg, 0.500 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (115 mg, 0.600 mmol) and 1-hydroxybenzotriazole (81 mg, 0.600 mmol) in dimethylformamide (3 ml)) was stirred at room temperature for 1.5 hours. Then, methanol (3 ml)) and triethylamine (153 ul, 1.10 mmol) were added, and the mixture was heated to reflux for 1.5 hours. The reaction solution was diluted with ethyl acetate, washed with an aqueous saturated sodium bicarbonate solution, a 10% aqueous citric acid solution, and an aqueous saturated sodium chloride solution, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure, and the residue was washed with diethyl ether to obtain 3-benzyloxy-5-(4-fluoro-benzylcarbamoyl)-4-hydroxy-pyridine-2-carboxylic acid methyl ester 14 (141 mg,69%) as a colorless crystal.

NMR (DMSO-d₆)δ: 3.85(3H, s), 4.52(2H, d, J=6.0 Hz), 5.15(2H, s), 7.13-7.21(2H, m), 7.31-7.47(7H, m), 8.33(1H, s), 10.41(1H, t, J=6.0 Hz), 12.59(1H, brs).

-   13) After 3-bromopropene (2.15 ml, 24.8 mmol) was added to a     solution of the compound 14 (6.79 g, 16.5 mmol), and cesium     carbonate (8.09 g, 24.8 mmol) in dimethylformamide (54 ml)), the     mixture was stirred at room temperature for 4.5 hours. To the     reaction solution was added an aqueous ammonium chloride solution,     and this was extracted with ethyl acetate, washed with water and an     aqueous saturated sodium chloride solution, and dried with anhydrous     sodium sulfate. The solvent was distilled off under reduced     pressure, and the residue was washed with diethyl ether to obtain     1-allyl-3-benzyloxy-5-(4-fluoro-benzylcarbamoyl)-4-oxo-1,4-dihydro-pyridine-2-carboxylic     acid methyl ester 15 (6.15 g, 83%) as a colorless crystal.

NMR (CDCl₃) δ: 3.76(3H, s), 4.54(2H, d, J=6.0 Hz), 4.60(2H, d, J=6.0 Hz), 5.20-5.37(2H, m), 5.25(2H, s), 5.80-5.93(1H, m), 6.98-7.04(2H, m), 7.31-7.35(7H, m), 8.45(1H, s), 10.41(1H, m).

-   14) To a solution of the compound 15 (7.6 g, 16.9 mmol) in     1,4-dioxane (228 ml)) was added an aqueous solution (38 ml)) of     potassium osmate dihydrate (372mg, 1.01 mmol), and sodium     metaperiodate (14.5 g, 67.6 mmol) was further added, followed by     stirring at room temperature for 2 hours. The reaction solution was     added to a vessel to which ethyl acetate (300 ml)) and water (300     ml)) had been added, while stirring. The organic layer was washed     with water, a 5% aqueous sodium hydrogen sulfite solution and an     aqueous saturated sodium chloride solution, and dried with anhydrous     sodium sulfate. The solvent was distilled off under reduced     pressure, and the residue was washed with diethyl ether to obtain     3-benzyloxy-5-(4-fluoro     -benzylcarbamoyl)-4-oxo-1-(2-oxo-ethyl)-1,4-dihydro-pyridine-2-carboxylic     acid methyl ester 16 (5.39 g, 71%) as a colorless crystal.

NMR (CDCl₃)δ: 3.74(3H, s), 4.60(2H, d, J=5.91 Hz), 4.87(2H, s), 5.27(2H, s), 6.98-7.04(2H, m), 7.30-7.40(7H, m), 8.39(1H, s), 9.58(1H, 5), 10.38(1H, s).

-   15) To a solution of the compound 16 (400 mg, 0.884 mmol) in     methylene chloride (12 ml)) were added 2-methoxyethylamine (77 ul,     0.884 mmol) and acetic acid (18 ul), and the mixture was stirred at     room temperature for 5 minutes. Thereafter, the reaction was     performed at 140° C. for 30 minutes in a microwave reaction     apparatus. The solvent was distilled off under reduced pressure, the     residue was subjected to silica gel column chromatography, and     fractions eluting with toluene-acetone were concentrated under     reduced pressure to obtain     9-benzyloxy-2-(2-methy-ethyl)-1,8-dioxo-1,8-dihydro2H-pyrid[1,2-a]pyrazine-7-carboxylic     acid 4-fluoro-benzylamide 17-1 (226 mg, 54%) as a yellow solid.

NMR (CDCl₃)δ: 3.35(3H, s), 3.65(2H, t, J=5.1 Hz), 3.97(2H, t, J=4.5 Hz), 4.63(2H, d, J=5.7 Hz), 5.28(2H, s), 6.56(2H, m), 7.01(2H, t, J=8.7 Hz), 7.38-7.30(5H, m), 7.65(2H, d, J=6.6 Hz), 10.63(1H, s).

-   16) To the compound 17-1 (140 mg, 0.293 mmol) was added     trifluoroacetic acid (1.4 ml)) under ice-cooling, and the mixture     was stirred at 0° C. for 5 minutes and, then, at room temperature     for 1.5 hours. The solvent was distilled off under reduced pressure,     and this was diluted with chloroform, and added to ice water. This     was washed with an aqueous, saturated sodium bicarbonate solution, a     10% aqueous citric acid solution and water, and dried with anhydrous     sodium sulfate. The solvent was distilled off under reduced     pressure, and the residue was recrystallized with methylene     chloride-ethanol to obtain Example A-1 (89mg, 79%) as a yellow     crystal. melting point: 223-224° C.

NMR (DMSO-d₆)δ: 3.25(3H, s), 3.58(2H, t, J=5.4 Hz), 3.92(2H, t, J=5.1 Hz), 4.53(2H, d, J=5.7 Hz), 6.87(1H, d, 6.3 Hz), 7.14(2H, t, J=9.0 Hz), 7.33-7.38(2H, m), 7.47(1H, d, J=6.0 Hz), 8.77(1H, s), 10.56(1H, t, J=6.0 Hz), 12.00(1H, brs).

-   17) The compound 17-1 (157 mg, 0.329 mmol) was dissolved in     dimethylformamide (18 ml)) and methanol (1 ml)), 10%     palladium-carbon powder (31 mg) was added, and the mixture was     stirred at room temperature for 20 hours under the hydrogen     atmosphere. The reaction solution was filtered with Celite, and the     filtrate was concentrated under reduced pressure, The residue was     dissolved in chloroform, this was filtered with Celite again, and     the filtrate was concentrated under reduced pressure, The residue     was recrystallized with methylene chloride-methanol to obtain     Example B1 (66 mg, 52%) as a browm crystal,

melting point: 197-199° C.

NMR (DMSO-d₆)δ:3.27(3H, s), 3.55(2H, t, J=5.1 Hz), 3.68(2H, t, J=5.1 Hz), 3.79(2H, s), 4.36(2H, s), 4.51(2H, d, J=5.7 Hz), 7.15(2H, t, J=8.7 Hz), 7.32-7.37(2H, m), 8.38(1H, s), 10.46(1H, t, J=5.4 Hz), 12.41(1H, s).

EXAMPLE C-1

-   1) A compound 33 was synthesized using 1-aminomethylcyclopentanol     hydroxyethylamine according to the method of synthesizing a compound     17-1.

1H-NMR (CDCl₃): 1.30-1.80(10H, m), 3.47(1H, d, J=11.4 Hz), 3.61(1H, d, J=11.4 Hz), 3.80-3.95(1H, m), 4.30(1H, dd, J=14.7, 3.0 Hz), 4.60(2H, d, J=5.7 Hz), 5.17-5.23(2H, m), 5.39(1H, d, J=9.9 Hz), 6.95-7.10(2H, m), 7.20-7.40(5H, m), 7.58(2H, d, J=7.2 Hz), 8.41(1H, s), 10.40(1H, s).

-   2) A compound 33-2 was synthesized using hydroxyethylamine according     to the similar method.

Compound 33-2) 5-Benzyloxy-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1-oxa-3a,8a-diaza-cyclopenta[b]naphthalene-7-carboxylic acid 4-fluorobenzylamide

1H-NMR (DMSO-d₆)δ: 3.48-3.58(1H, m), 3.73-3.86(1H, m), 3.97-4.10(2H, m), 4.20-4.30(1H, m), 4.46-4.60(2H, m), 4.85(1H, dd, J=12.3, 3.5 Hz), 5.40(1H, d, J=10.2 Hz), 5.18(1H, d, J=10.2 Hz), 5.28(1H, dd, J=10.2, 3.2 Hz), 7.10-7.20(2H, m), 7.23-7.40(5H, m), 7.50-7.73(2H, m), 8.60(1H, s), 10.22(1H, m).

-   3) Example C-1 was synthesized using a compound 33, according to the     method of synthesizing Example A-1.

Melting point: >300° C.

1H-NMR (DMSO-d₆)δ: 1.10-1.60(10H, 3.25(1H, d, J=11.4 Hz), 3.37(1H, d, J=11.4 Hz), 3.76(1H, t, J=10.5 Hz,), 4.30(2H, d, J=5.8 Hz), 4.66(1H, dd, J=12.2, 3.8 Hz), 5.22(1H, dd, J=3.8, 10.4 Hz), 6.90-6.96(2H, m), 7.10-7.15(2H, m), 8.25(1H, s), 10.10(1H, brs), 11.32(1H, brs).

The following compounds were synthesized using the similar method.

EXAMPLE C-2 5-Hydroxy-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1-oxa-3a,8a-diazacyclopenta[b]naphthalene-7carboxylic acid 4-fluorobenzylamide

Melting point: 272-274° C.

1H-NMR (DMSO-d₆)δ: 3.59-3.67(1H, m), 3.72-3.81(1H, m), 3.98-4.10(2H, m), 4.27-4.35(1H, m), 4.52(2H, d, J=7.2 Hz), 4.92(1H, dd, J=12,3, 12.3 Hz), 5.27(1H, dd, J=3.6, 9.9 Hz), 7.11-7.20(2H, m), 7.30-7.40(2H, m), 8.49(1H, s), 10.32(1H, t, J=5.6 Hz), 11.53(1H, s).

EXAMPLE C-3 5-Hydroxy -6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 259° C.

1H-NMR (DMSO-d₆)δ: 1.60-1.67(1H, m), 1.72-1.85(1H, m), 3.25(1H, td, J=12.8, 3.5 Hz), 3.86-3.93(1H, m), 4.06(1H, dd, J=11.4, 4.2 Hz), 4.44-4.57(5H, m), 5.28(1H, t, J=3.8 Hz), 7.13-7.18(2H, m), 7.33-7.37(2H, m), 8.51(1H, s), 10.36(1H, t, J=6.0 Hz), 12.47(1H, s).

EXAMPLE C4 5-Hydroxy-1-isopropyl-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1H-1,3a,8a-triaza-cyclopenta[b]naphthalene-7-carboxylic acid 4-fluoro-benzylamide

melting point 232-234° C.

NMR (DMSO-d₆)δ: 1.03(3H, d, 6.6 Hz), 1.14(3H, d, 6.6 Hz), 2.79-3.66(5H, m), 3.82(1H, t, 10.8 Hz), 4.51(3H, m), 4.90(1H, m), 7.15(2H, t, 9.0 Hz), 7.34(2H, m), 8.45(1H, s), 10.39(1H, t, 5.4 Hz), 11.60(1H, s).

EXAMPLE C-5 5-Hydroxy-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1H-1,3a,8a-triaza-cyclopenta[b]naphthalene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 256-258° C.

NMR (DMSO-d₆)δ: 3.00-3.55(5H, m), 3.96(1H, t, 11.4 Hz), 4,52(2H, d, 11.7 Hz), 4.76(2H, m), 7.16(2H, t, 8.7 Hz), 7.35(2H, m), 8.48(1H, s), 10.42(1H, t, 5.4 Hz), 11.91(1H, s).

EXAMPLE C-6 5-Hydroxy-6,10dioxo1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 255° C.

NMR (DMSO-d₆)δ: 1.60(1H, s), 2.75-3.16(4H, m), 4.52(2H, d, 6.0 Hz), 4.13-4.68(4H, m), 7.16(2H, 9.0 Hz, t), 7.34(2H, m), 10.42(1H, s), 10.44(1H, 6.0 Hz, t), 12.81(1H, s).

EXAMPLE C-7 1-(2Diethylamino-ethyl)-5-hydroxy-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1H-1,3a,8a-triaza-cyclopenta[b]naphthalene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 186-187° C.

NMR (DMSO-d₆)δ: 0.97(6H, t, 7.2 Hz), 2.42-2.91(10H, m), 3.44-3.87(5H, m), 4.23(1H, m), 4.51(2H, d, 5.7 Hz), 5.00(1H, m), 7.16(2H, t, 9.0 Hz), 7.33-7.37(2H, m), 8.43(1H, s), 10.39(1H, t, 5.7 Hz), 11.81(1H, s).

EXAMPLE C-8 1-Hydroxy2,11-dioxo-2,5,5a,7,8,9,10,11-octahydro-6-oxa-4a,10a-diaza-cyclohepta[b]naphthalene-3-carboxylic acid 4-fluoro-benzylamide

melting point: 242-244° C.

NMR (DMSO-d₆)δ: 1.40-2.00(4H, m), 3.20-3.30(1H, m), 3.66-3,77(2H, m), 4.14-4.23(1H, m), 4.38-4.41(1H, m), 4.52(2H, d, 6.3 Hz), 4.58-4.63(1H, m), 5.34(1H, brs), 7.15(2H, t, 9.0 Hz), 7.33-7.37(2H, m), 8.50(1H, s), 10.39(1H, brs), 12.14(1H, s).

EXAMPLE C-9 5-Hydroxy-1-(2-hydroxy-ethyl)-6,10-dioxo1,2,3,4,6,9,9 a,10-octabydro-1,4a,8 a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

NMR (DMSO-d₆)δ: 1.58-1.80(1H, m) 2.70-3.60(7H, m), 4.40-4.54(6H, m), 4.77-4.82(1H, m), 7.15(2H, t, 9.0 Hz), 7.33-7.38(2H, m), 8.52(1H, s), 10.43(1H, brs), 12.57(1H, s).

EXAMPLE C-10 1-Hydroxy-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalene-3-carboxylic acid 4-fluoro-benzylamide

melting point: 256° C.

NMR (DMSO-d₆)δ: 1.47-1.77(4H, m), 2,69-2.81(2H, m), 3.34-3.41(1H, m), 4.08-4.12(1H, m), 4.26-4.40(2H, m), 4.52(2H, d, J=6.0 Hz), 7.15(2H, t, 8.8 Hz), 7.33-7.36(2H, m), 8.43(1H, s), 10.46(1H, t, J=6.0 Hz), 12.68(1H, s).

EXAMPLE C-11 5-Hydroxy-1-(2-methoxy-ethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 147° C.

NMR (DMSO-d₆)δ: 1.56-1.74(2H, m), 2.53-2.58(1H, m), 2.66-3.10(4H, m), 3.18(3H, s), 3.41-3.39(2H, m), 4.37-4.52(5H, m), 4.73-4.80(1H, m), 7.15(2H, t, 8.8 Hz), 7.33-7.37(2H, m), 8.56(1H, s), 10.40(1H, t, J=6.0 Hz,), 12.62(1H, s).

EXAMPLE C-12 5-Hydroxy-1-(2-isopropoxy-ethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 151° C.

NMR (DMSO-d₆)δ: 1.02(6H, dd, J=4.0, 6.0 Hz), 1.56-1.67(2H, m), 2.53-2.58(1H, m), 2.74-3.04(4H, m), 3.18(3H, s), 3.41-3.52(3H, m), 4.41-4.59(5H, m), 4.79-4.83(1H, m), 7.15(2H, t, 8.8 Hz), 7.34-7.36(2H, m), 8.58(1H, s), 10.40(1H, t, J=6.0 Hz), 12.56(1H, s),

EXAMPLE C-13 5-Hydroxy-3,3-dimethyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 275-277° C.

NMR (DMSO-d₆)δ: 2.97(3H, s), 3.01(3H, s), 3.00-3.18(3H, m), 4.45-4.56(5H, m), 5.16(1H, s), 7.15(2H, t, J=9 Hz), 7.35(2H, dd, J=5.4 Hz, 8.7 Hz), 8.51(1H, s), 10.36(1H, t, J=5.7 Hz), 12.4(1H, s).

EXAMPLE C-14 1-Cyclohexyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid-4-fluoro-benzylamide

melting point: 275-277° C.

NMR (DMSO-d₆)δ: 1.22-1.70(2H, m), 2.50-3.02(3H, m), 4.45(4H, m), 4.52(2H, s), 4.78(1H, d, J=13.2 Hz), 7.16(2H, t, J=8.71 Hz), 7.35(2H, dd, J=5.7 Hz, 8.4. Hz), 8.62(1H, s), 10.52(1H, s), 12.55(1H, s).

EXAMPLE C-15 5-Hydroxy-1-isopropyl-6,10-dioxo-1,2,3,4,6,9,9a,10-oxtahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid-4-fluoro-benzylamide

melting point: 220° C.

NMR (DMSO-d₆)δ: 0.94(6H, d, J=9.6 Hz,), 1.53-1.67(2H, m), 2.92-3.30(3H, m), 4.32-4.40(4H, m), 4.52(2H, d, J=5.7 Hz), 4.89(1H, d, J=14.1 Hz), 7.16(2H, t, J=9.0 Hz), 7.35(2H, dd, J=6.3 Hz, 9.0 Hz), 8.61(1H, s), 10.46(1H, s), 12.55(1H, s).

EXAMPLE C-16 5-Hydroxy-3,3-dimethyl-6,10dioxo-10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 280° C.

NMR (DMSO-d₆)δ: 0.87(3H, s), 0.93(3H, s), 2.59-3.15(6H, m), 4.09-4.57(6H, m), 7.14(2H, d, J=9.0 Hz,), 7.34(2H, dd, J=5.4 Hz, 8.4 Hz), 8.42(1H, s), 10.46(1H, s), 12.77(1H, s),

EXAMPLE C-17 5-Hydroxy-1-(2-morpholin-4-yl-2-oxo-ethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 140° C.

NMR (DMSO-d₆)δ: 1.60(2H, m), 2.91-3.62(13H, m), 4.41(2H, m), 4.51(2H, d, J=4.8 Hz), 4.80(2H, m), 7.15(2H, t, J=8.7 Hz), 7.34(2H, m), 8.44(1H, s), 10.43(1H, s), 12.54(1H, s).

EXAMPLE C-18 1-(3-Acetylamino-propyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 177-178° C.

NMR (DMSO-d₆)δ: 1.74(3H, s), 1.49-2.98(9H, m), 3.60(1H, s), 4.25-4.65(7H, m), 7.14(2H, t, J=8.4 Hz), 7.34(2H, m), 7.71(1H, s), 8.26(1H, s), 10.60(1H, s).

EXAMPLE C-19 1-Dimethycarbamoylmethyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 190° C.

NMR (DMSO-d₆)δ: 1.60(2H, m), 2.76(3H, s), 2.83(3H, s), 2.90-3.59(5H, s), 4.40(2H, m), 4.51(2H, d, 5.7 Hz), 4.80(1H, d, d=14.4 Hz), 4.98(1H, s), 7.16(2H, t, J=8.4 Hz), 7.34(2H, m), 8.54(1H, s), 10.42(1H, s).

EXAMPLE C-20 5-Hydroxy-1-(3-methanesulfonylamino-propyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 176° C.

NMR (DMSO-d₆)δ: 1.54-1.75(4H, m), 2.80(3H, s), 2.30-3.04(8H, m), 4.45(2H, m), 4.52(2H, d, J=5.6 Hz), 4.75(1H, d, J=13.2 Hz), 6.91(1H, t, J=5.6 Hz), 7.16(2H, t, J=8.8 Hz), 7.36(2H, m), 8.61(1H, S), 10.41(1H, t, J=5.6 Hz), 12.58(1H, s).

EXAMPLE C-21 5-Hydroxy-2-methyl-6,10-dioxo-3,4,6,9,9a,10-bexabydro-2H-1-oxa-4a,8a-dizazaanthracene-7-carboxylic acid 4-fluorobenzylamide

NMR (CDCl3)δ: 1.27(3H, d, J=6.0 Hz), 1.55-1.78(2H, m), 3.11(1H, td, J=12.9, 8.7 Hz), 3.89-4.00(1H, m), 4.16(1H, dd, J=13.8, 3.9 Hz), 4.34(1H, dd, J=13.8, 3.9 Hz), 4.60(2H, d, J=6.0 Hz), 4.71(1H, ddd, J=13.5, 4.8, 1.8 Hz), 5.08(1H, t, J=3.9 Hz), 6.96-7.04(2H, m), 7.26-7.35(2H, m), 8.82(1H, s), 10.41(1H, br s), 12.41(1H, br s).

EXAMPLE F-1 5-Hydroxy-1-isobutyl-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1H-1,3a,8a-triazacyelopenta[b]naphthalene-7-carboxylic acid-4-fluorobenzylamide

-   1) According to the method of synthesizing a compound 17-1, the     crude purified product (503 mg) of a compound 48 was obtained at a     yield of 82% from a compound 16 (600 mg). -   2) To a solution of a compound 48 (100 mg, 0.22 mmol),     isobutylaldehyde (39 μl, 0.482 mmol) and acetic acid (25 μl, 0.432     mmol) in dichloromethane (4 ml)) was added. sodium     triacetoxyborohydride (92 mg, 0.432 mmol) under ice-cooling, and the     mixture was stirred at room temperature for 2 hours, Further,     isobutylaldehyde (20 μl) and sodium triacetoxyborohydride (46 mg)     were added, and the mixture was stirred for 30 minutes. To the     reaction solution was added water, this was extracted with     chloroform, and the organic layer was washed with an aqueous     saturated sodium bicarbonate solution. After dying, the solvent was     distilled off under reduced pressure, and this was purified by     silica gel column chromatography. A compound 49 (87 mg) was obtained     as a colorless crystal at a yield of 78%.

1H-NMR (CDCl₃)δ: 0.96(3H, d, J=6.6 Hz), 0.97(3H, d, J=6.3 Hz), 1.72-1.86(1H, m), 2.25-2.41(2H, m), 2.47-2.58(1H, m), 3.39-3.46(1H, m), 3.69-3.76(2H, m), 3.85-3.93(1H, m), 4.06(1H, dd, J=9.9, 2.7 Hz), 4.16-4.22(1H, m), 4.57(1H, dd, J=15.3, 5.1 Hz), 4.64(1H, dd, J=14.7, 5.1 Hz), 5.20(1H, d, J=9.9 Hz), 5.38(1H, d, J=9.9 Hz), 6.96-7.05(2H, m), 7.28-7.36(5H, m), 7.58-7.62(2H, m), 8.40(1H, s), 10.44(1H, br s).

-   3) According to the method of a step 17) of Example B-1, a compound     F-1 (43 mg) was obtained at a yield of 64% from a compound 49 (81     mg).

1H-NMR (DMSO-d₆)δ: 0.90(3H, d, J=6.4 Hz,), 0.91(3H, d, J=6.01 H z), 1.75-1.84(1H, m), 2.24-2.39(1H, m), 2.39-2.54(2H, m), 3.36-3.43(1H, m), 3.52-3.60(1H, m), 3.67-3.73(1H, m), 3.81-3.88(1H, m), 4.19-4.23(1H, m), 4.52(2H, d, J=6.0 Hz), 4.94-4.99(1H, m), 7.12-7.20(2H, m), 7.32-7.38(2H, m), 8.45(1H, s), 10.37(1H, t, J=2.0 Hz), 11.74(1H, s).

According to the same manner as that of Example F-1, the following Example compounds F-2 to F-63 were synthesized.

EXAMPLE F-2 5-Hydroxy-1-isobutyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point 146-148° C.

1H-NMR (DMSO-d₆)δ: 0.63(3H, d, J=6.6 Hz), 0.79(3H, d, J=6.6 Hz), 1.56-1.66(2H, m), 1.67-1.75(1H, m), 1.94-1.99(1H, m), 2.41-2.54(2H, m), 2.96-3.06(2H, m), 4.41-4.59(5H, m), 4.76-4.81(1H, m), 7.14-7.21(2H, 7.33-7.38(2H, m), 8.61(1H, s), 10.40(1H, d, J=5.8 Hz), 12.56(1H, s).

EXAMPLE F-3 1-Cyclopropylmethyl-5-hydroxy6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8atriaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 182-184° C.

NMR (DMSO-d₆)δ: 0.06(2H, m), 0.43(2H, d, 8.4 Hz), 0.80(1H, m), 1.66(2H, m), 2.28-3.30(4H, m), 4.40-4.50(4H, m), 4.52(2H, d, 4.78(2H, m), 7.15(2H, t, 8.7 Hz), 7.34(2H, m), 8.55(1H, s), 10.47(1H, s), 12.55(1H, s).

EXAMPLE F-4 1-Cyclopentylinethyl-5-hydroxy-6,1-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 184-185° C.

NMR (DMSO-d₆)δ: 0.88-2.10(1H, m), 2.60(2H, m), 2.95-3.28(2H, m), 4.38-4.53(6H, m), 4.82(1H, m), 7.15(2H, t, 9.0 Hz), 7.34(2H, m), 8.57(1H, s), 10.42(1H, s), 12.45(1H, s).

EXAMPLE F-5 5-Hydroxy-1-(4-methylsulfanylbenzyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 1.51-1.56(1H, m), 1.69-1.74(1H, m), 2.42(3H, s), 2.55-2.62(1H, m), 2.80-2.84(1H, m), 3.00-3.08(1H, m), 3.32-3.36(1H, m), 3.93(1H, d, J=13.6 Hz), 4.45-4.53(4H, m), 4.58(1H, s), 4.83(1H, d, J=1.5.2 Hz), 7.11-7.19(6H, 7.33-7.40(2H, m), 8.34(1H, s), 10.38(1H, t, J=6.0 Hz), 12.58(1H, s).

EXAMPLE F-6 1-(5-Chloro-1,3-dimethyl-1H-pyrazol-4-ylmethyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,1 0-octahydro-1,4a,8a-taliaz,aantliracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 1.56-1.59(2H, m), 1.88(3H, s), 2.37-2.45(1H, m), 2.76-2.80(1H, m), 3.00-3.06(2H, m), 3.64(3H, s), 3.87(1H, d, J=13.2 Hz), 4.40-4.55(5H, m), 4.97(1H, d, J=14.4 Hz), 7.13-7.19(2H, m), 7.33-7.38(2H, m), 8.56(1H, s), 10.39(1H, t, J=6.0 Hz), 12.46(1H, s).

EXAMPLE F-7 5-Hydroxy-1-(3-methoxybenzyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-flnorobenzylamide

(DMSO-d₆)δ: 1.52-1.57(1H, m), 1.70-1.80(1H, m), 2.60-2.68(1H, m), 2.84-2.90(1H, m), 3.01-3.09(1H, 3.36(1H, d, J=14.0 Hz), 3.61(3H, s), 3.91(1H, d, J=14.0 Hz), 4.45-4.52(4H, m), 4.58(1H, s), 4.76(1H, d, J=14.8 Hz), 6.68-6.73(2H, m), 6.77(1H, d, J=7.6 Hz), 7.13-7.19(3H, m), 7.33-7.38(2H, m), 8.17(1H, s), 10.38(1H, t, J=6.0 Hz), 12.57(1H, s).

EXAMPLE F-8 5-Hydroxy-1-(4-methanesulfonylbenzyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 1.54-1.58(1H, m), 1.74-1.80(1H, m), 2.67-1.74(1H, m), 2.83-2.87(1H, m), 3.05-3.12(1H, m), 3.18(3H, s), 3.52(1H, d, J=14.8 Hz), 4.09(1H, d, J=14.8 Hz), 4.46-4.52(4H, m), 4.67(1H, s), 4.73(1H, d, J=14.8 Hz), 7.12-7.1.8(2H, m), 7.32-7.36(2H, m), 7.46(2H, m), 7.80(2H, d, J=8.0 Hz), 8.17(1H, s), 10.37(1H, t, J=5.8 Hz), 12.59(1H, s).

EXAMPLE F-9 5-Hydroxy-1-(6-methoxypyridin-3-ylmethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 1.51-1.56(1H, m), 1.71-1.77(1H, m), 2.58-2.66(1H, m), 2.80-2.86(1H, m), 3.01-3.09(1H, m), 3.38(1H, d, J=13.6 Hz), 3.78(3H, s), 3.87(1H, d, J=13.6 Hz), 4.45-4.52(4H, m), 4.60(1H, s), 4.82(1H, d, J=13.6 Hz), 6.71(1H, d, J=8.6 Hz), 7.12-7.19(2H, m), 7.33-7.38(2H, m), 7.49(1H, d, J=8.6 Hz), 7.98(1H, s), 8.30(1H, s), 10.37(1H, t, J=6.0 Hz), 12.58(1Hz, s).

EXAMPLE F-10 5-Hydroxy-1-isobutyl-3,3-dimethyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 0.64(3H, d, J=6.4 Hz), 0.82(3H, d, J=6.8 Hz), 0.90(3H, s), 0.91(8H, s), 1.59-1.67(1H, m), 1.92-1.97(1H, m), 2.11-2.15(1H, m), 2.51-2.57(1H, m), 2.67(1H, d, J=12.0 Hz), 2.77(1H, d, J=12.8 Hz), 4.13(1H, s), 4.21(1H, d, J=12.8 Hz), 4,47-4.59(3H, s), 4.80(1H, dd, J=14.4, 2.8 Hz), 7.14-7.19(2H, m), 7.34-7.38(2H, m), 8.66(1H, s), 10.41(1H, t, J=6.0 Hz), 12.44(1H, s).

EXAMPLE F-11 5-Hydroxy-1,3,3-trimethyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 0.89(6H, s), 2.14-2.18(1H, m), 2.24(3H, s), 2.54-2.58(1H, m), 2.74-2.78(1H, s), 3.88(1H, s), 4.21(1H, d, J=13.2 Hz), 4.45-4.53(3H, m), 4.72-4.76(1H, m), 7.13-7.19(2H, m), 7.33-7.38(2H, m), 8.64(1H, s), 10.40(1H, t, J=6.0 Hz), 12.46(1H, s).

EXAMPLE F-12 4-[7-(4-Fluorobenzylcarbamoyl)-5-hydroxy-6,10-dioxy-3,4,6,9,9a,10-hexahydro-2H-1,4a,8a-triazaanthracene-1-yl]butanoic acid ethyl ester

(CDCl₃)δ: 1.23(3H, t, J=7.1 Hz), 1.70-1.79(1H, m), 1.86-2.00(1H, m), 2.17-2.34(2H, m), 2.46-2.57(1H, m), 2.61-2.77(2H, m), 2.85-2.92(1H, m), 3.13-3.18(1H, m), 4.13(2H, q, J=7.1 Hz), 4.27-4.34(2H, m), 4.57-4.63(3H, m), 4.66-4.73(1H, m), 6.95-7.03(2H, m), 7.29-7.36(2H, m), 8.36(1H, s), 10.48(1H, t, J=4.8 Hz), 12.50(1H, s).

EXAMPLE F-13 1-(3-Dimethylcarbamoylpropyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a, 8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(CDCl₃)δ: 1.62-1.82(3H, m), 1.83-2.00(1H, m), 2.10-2.35(2H, m), 2.57-2.65(2H, m), 2.75-2.95(2H, m), 2.92(3H, s), 2.96(3H, s), 3.07-3.14(1H, m), 4.23-4.30(2H, m), 4.60(2H, d, J=6.0 Hz), 4.68(1H, dd, J=13.2, 4.5 Hz), 5.12(1H, d, J=12.6 Hz), 6.95-7.02(2H, m), 7.28-7.35(2H, m), 8.42(1H, s), 1054(1H, t, J=5.4 Hz), 12.51(1H, s).

EXAMPLE F-14 5-Hydroxy (4-morpholin-4-yl-4-oxobutyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(CDCl₃)δ: 1.61-1.83(3H, m), 1.84-2.00(1H, m), 2.12-2.23(1H, m), 2.25-2.36(1H, m), 2.56-2.64(2H, m), 2.75-2.95(2H, m), 3.09-3.15(1H, m), 3.37(2H, t, J=4.8 Hz), 3.61-3.66(6H, m), 4.26-4.32(2H, m), 4.59(2H, d, J=5.7 Hz), 4.68(1H, dd, J=13.2, 4.5 Hz), 4.95-5.01(1H, m), 6.95-7.03(2H, m), 7.28-7.35(2H, m), 8.40(1H, s), 10.52(1H, t, J=5.7 Hz), 12.51(1H, s).

EXAMPLE F-15 5-Hydroxy-1-methyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 252-253° C.

(DMSO-d₆)δ: 1.56-1.75(2H, m), 2.22(3H, s), 2.50-2.55(1H, m), 2.90-3.10(2H, m), 4.17(1H, brs), 4.39-4.42(2H, m), 4.52(2H, J=6.0 Hz), 4.74-4.78(1H, m), 7.13-7.17(2H, m), 7.33-7.37(2H, m), 8.61(1H s,), 10.40(1H, t, J=6.0 Hz), 12.54(1H, s).

EXAMPLE F-16 5-Hydroxy-6,10-dioxo-1-thiophen -3-ylmethyl-1,2,3,4,6,9,9a,10-octahydro-3,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 242-243° C.

(DMSO-d₆)δ: 1.52-1.73(2H, m), 2.69-2.62(1H, m), 2.87-3.03(2H, m), 3,52(1H, d, J=13.6 Hz), 3.90(1H, d, J=14.4 Hz), 4.40-4.56(5H, m), 4.83-4.90(3H, m), 6.92(1H, d, J=5.2 Hz), 7.13-7.17(2H, m), 7.28-7.37(3H, m), 7.42-7.44(1H, m), 8.46(1H, s), 10.39(1H, t, J=6,0 Hz), 12.58(1H, s).

EXAMPLE F-17 5-Hydroxy-6,10-dioxo-1-thiazol-2-ylmethyl-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point 214-215° C.

(DMSO-d₆)δ: 1.54-1.72(2H, m), 2.75-2.81(1H, m), 2.95-3.07(2H, m), 3.80(1H, d, J=16.0 Hz), 4.37(1H, d, J=16.4 Hz), 4.44-4.51(4H, m), 4.69(1H, brs), 4.89-4.93(1H, m), 7.13-7.17(2H, m), 7.32-7.35(2H, m), 7.55(1H, d, J=3.2 Hz), 7.69(1H, d, J=3.2 Hz), 8.37(1H, s), 10.36(1H, t, J=6.0 Hz), 12.50(1H, s).

EXAMPLE F-18 5-Hydroxy-(3-methylsulfanyl-propyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 162-164° C.

(DMSO-d₆)δ: 1.50-1.82(4H, m), 2.27(3H, s), 2.32-2.44(3H, m), 2.60-2.82(2H, m), 3.00-3.14(2H, 4.37-4.59(5H, m), 4.75-4.79(1H, m), 7.13-7.17(2H, m), 7.33-7.35(2H, m), 8.60(1H, s), 10.40(1H, t, J=6.0 Hz), 12.57(1H, s).

EXAMPLE F-19 5 -Hydroxy-6,10-dioxo-1-pyridin-4-ylmethyl-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 180-183° C.

(DMSO-d₆)δ: 1.52-1.76(2H, m), 2.62-2.80(2H, m), 3.01-3.07(1H, m), 3.42(1H, d, J=1.5.2 Hz), 4.05(1H, d, J=15.2 Hz, 4.49-4.50(4H, m), 4.64(1H, brs), 4.78-4.81(1H, m), 7.12-7.21(4H, m), 7.32-7.36(2H, m), 8.33(1H, s), 8.42(2H, d, 10.39(1H, t, J=6.0 Hz), 12.55(1H, s).

EXAMPLE F-20 1-Cyclohexylmethyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7carboxylic acid 4-fluorobenzylamide

melting point: 201-202° C.

(DMSO-d₆)δ: 0.56-0.59(1H, m), 0.87-0.84(1H, 1.02-1.13(3H, m), 1.23-1.29(1H, m), 1.49-1.70(6H, m), 1.92-1.97(1H, m), 2.52-2.55(1H, m), 2.96-3.03(2H, m), 4.40-4.43(3H, m), 4.52(2H, d, J=6.0 Hz), 4.73-4.77 (1H, m), 7.12-7.16(2H, m), 7.32-7.36(2H, m), 8.59(1H s,), 10.40(1H, t, J=5.2 Hz), 12.58(1H, s).

EXAMPLE F-21 5-Hydroxy-6,10-dioxo-1-pyridin-2-ylmethyl-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 216-219° C.

(DMSO-d₆)δ: 1.52-1.76(2H, m), 2.66-2.80(1H, m), 2.90-3.07(2H, m), 3.67(1H, d, J=15.2 Hz), 4.01(1H, d, J=13.2 Hz), 4.37-4.97(4H, m), 4.62(1H, brs), 4.85-4.88(1H, m), 7.07-7.25(4H, m), 7.33-7.36(2H, 7.64-7.68(1H, m), 8.26(1H, s), 8.45(1H, s), 10.36(1H, 12.57(1H, s).

EXAMPLE F-22 1-(2-Ethyl-butyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octalaydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 137-140° C.

(DMSO-d₆)δ: 0.62(3H, t, J=7.2 Hz), 0.77(3H, t, J=7.2 Hz), 0.99-1.30(5H, m), 1.57-1.71(2H, m), 1.97-2.02(1H, m), 2.44-2.58(2H, m), 3.02-3.32(2H, m), 4.34-4.57(5H, m), 4.78-4.82(1H, m), 7.13-7.17(2H, m), 7.32-7.36(2H, m), 8.60(1H, s), 10.39(1H, t, J=5.2 Hz), 12.54(1H, s).

EXAMPLE F-23 5-Hydroxy-1-(2-morpholin-4-ylethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 254-256° C.

(DMSOd₆)δ: 1.55-1.68(2H, m), 2.28-2.39(8H, m), 2.59-2.65(1H, m), 2.82-3.09(3H, m), 3.33-3.58(5H, m), 4.34-4.50(3H, m), 4.52(2H, d, J=6.2 Hz), 4.79-4.84(1H, m), 7.12-7.17(2H, m), 7.32-7.36(2H, m), 8.52(1H, s), 10.45(1H, t, J=5.2 Hz), 12.55(1H, s).

EXAMPLE F-24 1-Hydroxy-6-methyl-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naplatbalene-3-carboxylic acid 4-fluorobenzylamide

melting point: 255° C.

(DMSO-d₆)δ: 1.48-1.55(1H, m), 1.67-1.80(3H, m), 2.29(3H, s), 2.75-2.80(2H, m), 3.23-3.31(1H, m), 4.07-4.09(1H, m), 4.36-4.40(1H, m), 4.45-4.59(3H, m), 4.68-4.69(1H, m), 7.13-7.17(2H, m), 7.30-7.37(2H, m), 8.50(1H, s), 10.42(1H, t, J=6.0 Hz), 12.42(1H, s).

EXAMPLE F-25 1-Hydroxy-6isobutyl-2,11-2,5a,6,7,8,9,10, 11-octahydro-5H-4a,6,10a-triaza-cyclo hepta[b]napbthalene-3-carboxylic acid 4-fluorobenzylamide

melting point: 221-223° C.

DMSO-d₆)δ: 0.81(3H, d, J=6.8 Hz), 0.84(3H, d, J=6.4 Hz), 1.45-1.78(5H, m), 2.36-2.54(2H, m), 2.27-2.93(2H, m), 3.17-3.23(1H, m), 4.03-4.06(1H, m), 4.32-4.56(4H, m), 4.82-4.85(1H, m), 7.13-7.17(2H, m), 7.30-7.37(2H, m), 8.48(1H, s), 10.42(1H, t, J=6.0 Hz), 12.53(1H, s).

EXAMPLE F-26 6-Cyclopropylmethyl-1-hydroxy-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalene3-carboxylic acid 4-fluorobenzylamide

melting point: 213° C.

DMSO-d₆)δ: 0.15-0.26(2H, m), 0.46-0.48(2H, m), 0.86-1.06(1H, m), 1.45-1.75(4H, m), 2.45-2.65(1H, m), 2.68-2.83(1H, m), 2.91-2.98(2H, m), 3.17-3.26(1H, m), 4.08-4.14(1H, m), 4.43-4.45(2H, m), 4.54(2H, d, J=5.6 Hz), 4.89-4.91(1H, m), 7.15-7.19(2H, m), 7.35-7.39(2H, m), 8.50(1H, s), 10.47(1H, t, J=6.0 Hz), 12.52(1H, s).

EXAMPLE F-27 1-Furan-2-ylmethyl-5-hydroxy-6,10-dioxo1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylarnicle

melting point: 193-197° C.

DMSO-d₆)δ: 1.67(2H, m), 2.61(3H, s), 2.93(2H, m), 3.75(1H, d, J=14.8 Hz), 3.84(1H, d, J=1.4.8 Hz), 4.34-4.47(3H, m), 4.52(2H, d, J=6.0 Hz), 4.96(1H, d, J=14.8 Hz), 6.36(2H, s), 7.16(2H, t, J=8.8 Hz), 7.35(2H, m), 7.59(1H s,), 8.97(1H s,), 10.4.3(1H, s), 12.51(1H, s).

EXAMPLE F-26 1-(4-Dimethylamino-benzyl)-5-hydroxy6,10dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 221-223° C.

DMSO-d₆)δ: 1.55-1.99(2H, m), 2.87(6H, s), 2.87-3.06(4H, m), 3.80(1H, d, J=14.0 Hz), 4.50(5H, m), 4.83(1H, d, J=14.0 Hz), 6.58(2H, d, 6.98(2H, d, J=8.8 Hz), 7.15(2H, t, 7.35(2H, m), 8.31(1H s,), 10.39(1H s,), 12.58(1H, s).

EXAMPLE F-29 5-Hydroxy-6,10-dioxo-1-(4-trifluoromethyl-benzyl)-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 273-277° C.

DMSO-d₆)δ: 1.52-1.70(2H, m), 2.63-3.04(3H, m), 3.50(1H, d, 4.10(1H, d, J=14.8 Hz), 4.54(5H, m), 4.79(1H, d, J=14.8 Hz), 7.14(2H, t, J=8.8 Hz), 7.33(2H, m), 7.55(2H, d, J=6.8 Hz), 7.61(2H, d, J=8.0 Hz), 8.22(1H, s), 10.40(1H, s), 12.56(1H, s).

EXAMPLE F-30 5-Hydroxy-6,10-dioxo-1-pyridin-3-ylmethyl-1,2,3,4,6,9,9a,10-octahydro 1,4a,8atriaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 210-212° C.

DMSO-d₆)δ: 1.51-.1.76(2H, m), 2.63(1H, t, J=12.8 Hz), 2.80(1H, d, J=12.0 Hz), 3.07(1H, t, J=12.8 Hz), 3.44(1H, d, J=13.2 Hz), 4.00(1H, d, 14.0 Hz), 4.47(4H, m), 4.62(1H, s), 4.84(1H, d, J=14.0 Hz), 7.16(2H, t, J=8.8 Hz), 7.33(2H, m), 7.58(1H, d, J=7.6 Hz), 8.30(1H, s), 8.45(2H, s), 10.41(1H, s), 12.57(1H, s).

EXAMPLE F-31 1-(2-Chloro-6-fluoro-benzyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-flnorobenzylamide

melting point: 213-215° C.

DMSO-d₆)δ: 1.58(2H, 2H), 2.55-3.09(3H, m), 3.45(1H, d, J=12.4 Hz), 4.16(1H, d, J=12.4 Hz), 4.40-4.58(4H, m), 5.12(1H, d, J=14.4 Hz), 7.15-7.38(7H, m), 8.66(1H, s), 10.41(1H, t, J=6.4 Hz), 12.46(1H, s).

EXAMPLE F-32 5-Hydroxy-1-(4-methoxy-benzyl) -6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 191-193° C.

NMR (DMSO-d₆)δ:1.50-1.77(2H, m), 2.58-3.06(3H, m), 3.68(31-I, a), 3.88(1H, d, J=13.6 Hz), 4.41-4.55(4H, m), 4.80(2H, d, J=14.4 Hz), 6.80(2H, d, J=8.8 Hz), 7.09(2H, d, J=8.4 Hz), 7.15(2H, t, J=8.8 Hz), 7.35(2H, m), 8.28(1H, s), 10.48(1H, s), 12.58(1H, s).

EXAMPLE F-33 1-(3,5-Bis-trifluoromethyl-benzyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 275-277° C.

NMR (DMSO-d₆)δ:1.58-1.88(2H, m), 2.51-3.14(3H, m), 3.33-4.10(3H, m), 4.51(2H, m), 4.73(1H, m), 7.15(2H, m), 7.34(2H, m), 7.82-7.93(4H, m), 10.31(1H, s), 12.57(1H, s).

EXAMPLE F-34 1-(4-Diethylamino-benzyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fltiorobenzylamicle

melting point: 182° C.

NMR (DMSO-d₆)δ: 1.04(6H, t, J=6.8 Hz), 1.50-1.69(2H, m), 2.55-3.05(3H, m), 3.26(4H, q, J=7.2 Hz), 3.80(1H, d, J=1.3.6 Hz), 4.44-4.57(4H, m), 4.91(1H, d, J=12.4 Hz), 6.52(2H, d, J=8.8 Hz), 6.94(2H, d, J=8.4 Hz), 7.15(2H, t, J=8.4 Hz), 7.35(2H, m), 8.46(1H, s), 10.41(1H, s), 12.60(1H, s).

EXAMPLE F-35 5-Hydroxy-1-((E)-2-methyl-but-2-enyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 175-177° C.

NMR (DMSO-d₆)δ: 1.35(3H, s), 1.51(3H, d, J=6.0 Hz), 1.52-1.69(3H, m), 2.60-3.15(3H, m), 4.31-4.52(5H, m), 4.67-4.76(1H, m), 5.30-5.40(1H, m), 7.15(2H, t, J=8.41 Hz), 7.28-43(2H, m), 8.46(1H, s), 10.39(1H, brs), 12.60(1H, s).

EXAMPLE F-36 1-(3-Dimethylamino-2-methyl-propyl)-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

NMR (DMSO-d₆)δ: 0.63-0.68(2H, m), 1.57-1.82(3H, m), 2.11-2.49(10H, m), 2.98-3.11(2H, m), 4.41-4.54(5H, m), 4.73-4.80(1H, m), 7.14-7.18(2H, m), 7.31-7.38(2H, m), 8.58(1H, s), 10.40(1H, s), 12.57(1H, s).

EXAMPLE F-37 1-(3,3-Dimethyl-butyl)-5-hydroxy-6,10-dioxo-1.,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 175-177° C.

NMR (DMSO-d₆)δ: 1.19-1.36(2H, m), 1.57-1.70(2H, m), 2.23-2.30(1H, m), 2.51-2.69(2H, m), 2.97-3.04(2H, m), 4.42-4.54(5H, m), 4.78(1H, d, J=14.0 Hz), 7.13-7.17(2H, m), 7.33-7.36(2H, m), 8.63(1H, s), 10.39(1H, t, J=6.0 Hz), 12.56(1H, s).

EXAMPLE F-38 1-Ethyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluoro-benzylanaide

melting point: 221° C.

NMR (DMSO-d₆)δ: 0.94(3H, t, 1.56-1,71(2H, m), 2.45-2.50(1H, m), 2.59-2.76(2H, m), 2.96-3.03(2H, m), 4.40-4.44(3H, m), 4.52(2H, d, J=6.0 Hz), 4.77-4.82(1H, m), 7.14-7.18(2H, m), 7.34-7.38(2H, m), 8.62(1H, s), 10.41(1H, t, J=6.0 Hz), 12.59(1H, s).

EXAMPLE F-39 5-Hydroxy-6,10-dioxo-1-(2-oxo-propyl)-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 244-246° C.

NMR (DMSO-d₆)δ: 1.54-1.61(1H, m), 1.67-1.76(1H, m), 2.22(3H, s), 2.50-2.56(1H, m), 2.91-3.02(2H, m), 4.18(1H, s), 4.38-4.15(2H, m), 4.52(2H, d, J=6.0 Hz), 4.76(1H, d, J=14.4 Hz), 7.13-7.18(2H, m), 7.34-7.87(2H, m), 8.61(1H s), 10.40(1H, t, J=6.0 Hz), 12.54(1H, s).

EXAMPLE F-40 5-Hydroxy-6,10-dioxo-1-(4,4,4-trifluoro-butyl)-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 220° C.

NMR (DMSO-d₆)δ: 1.53-1.62(2H, m), 1.67-1.75(1H, 2.07-2.18(2H, m), 2.40-2.47(1H, m), 2.64-2.78(2H, m), 2.96-3.04(2H, m), 4.42-4.49(2H, m), 4.53(2H, d, J=5.2 Hz), 4.74(1H, d, J=12.8 Hz), 7.13-7.17(2H, m), 7.33-7.37(2H, m), 8.61(1H, s), 10.40(1H, t, J=6.0 Hz), 12.57(1H, s).

EXAMPLE F-41 5-Hydroxy-1-(3-methyl-butyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluorobenzylamide

melting point: 151° C.

NMR (DMSO-d₆)δ: 0.78(6H, dd, J=7.6, 16.2 Hz), 1.21-1.28(2H, m), 1.41-1.48(1H, m), 1.56-1.71(2H, m), 2.22-2.31(1H, m), 2.51-2.59(1H, m), 2.66-2.73(1H, m), 2.96-3.05(2H, m), 4.41-4.55(5H, m), 4.80(1H, d, J=13.2 Hz), 7.13-7.18(2H, 7.33-7.37(2H, m), 8.64(1H, s), 10.40(1H, t, J=6.0 Hz), 12.57(1H, s).

EXAMPLE F-42 5-Hydroxy-1-isobutyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 180-182° C.

NMR (DMSO-d₆)δ: 0.62(3H, d, J=6.0 Hz), 0.78(3H, d, J=6.4 Hz), 1.55-1.69(3H, m), 1.93-1.99(1H, m), 2.97-3.08(2H, m), 4.39-4.46(3H, m), 4.59-4.64(2H, m), 4.75-4.81(1H, m), 7.16-7.23(1H, m), 7.27-7.34(1H, m), 7.47-7.53(1H, m), 8.59(1H s,), 10.44(1H, s), 12.57(1H, s).

EXAMPLE F-43 1-Cyclopropylmethyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 189-192° C.

NMR (DMSO-d₆)δ: 0.00-0.10(2H, m), 0.35-0.41(2H, m), 0.70-0.77(1H, m), 1.57-1.69(2H, m), 2.52-2.65(1H, m), 2.67-2.85(1H, m), 2.91-2.99(1H, m), 4.30-4.41(2H, m), 4.48-4.52(2H, m), 4.71-4.80(1H, m), 7.06-7.10(1H, m), 7.18-7.22(1H, m), 7.36-7.40(1H, m), 8.62(1H, s), 10.30(1H, s), 12.26(1H, s).

EXAMPLE F-44 1-Furan-2-ylmethyl-5-hydroxy-6,10-clioxo-1,2,3,4,6,9,9a, 10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 190-192° C.

NMR (DMSOd₆)δ: 1.56-1.68(2H, m), 2.54-2.63(1H, m), 2.89-2.99(2H, m), 3.80(2H, dd, J=18.4, 33.2 Hz), 4.37-4.51(3H, m), 4.62(2H, d, J=6.0 Hz), 4.97(1H, d, J=15.2 Hz), 6.39(2H, s), 7.18-7.22(1H, m), 7.31-7.34(1H, m), 7.48-7.51(1H, m) 7.58(1H s,), 8.64(1H, s), 10.45(1H, t, J=6.0 Hz), 12.55(1H, s).

EXAMPLE F-45 5-Hydroxy-6,10-dioxo-1-thiazol-2-ylmethyl-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 217-219° C.

NMR (DMSO-d₆)δ: 1.59-1.74(2H, m), 2.76-2.83(1H, m), 2.97-3.08(2H, m), 3.90(1H, d, J=16.0 Hz), 4.36(1H, d, J=16.0 Hz), 4.46-4.69(5H, m), 4.89(1H, d, J=14.8 Hz), 7.18-7.22(1H, 7.28-7.31(1H, m), 7.47-7.53(1H, m), 7.54(1H, d, J=3.2 Hz), 7.68(1H, d, J=3.2 Hz), 8.34(1H, s), 10.40(1H, d, 12.52(1H, s).

EXAMPLE F-46 5-Hydroxy-6,10-dioxo-1-pyridin-2-ylmethyl-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 190-193° C.

NMR (DMSO-d₆)δ: 1.54-1.61(1H, m), 1.69-1.75(1H, m), 2.66-2.74(1H, m), 2.91-3.08(2H, m), 3.68(1H, d, J=14.4 Hz), 4.02(1H, d, J=14.8 Hz), 4.40-4.67(5H, m), 4.85(1H, d, J=12.4 Hz), 7.16-7.35(3H, m), 7.46-7.52(1H, m), 7.61-7.69(1H, m), 8.20(1H, s), 8.43-8.47(1H, m), 10.41(1H, d, J=6.0 Hz), 12.58(1H, s).

EXAMPLE F-47 5-Hydroxy-1-isobutyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 2,4-dilluoro-benzylamide

melting point: 194° C.

NMR (DMSO-d₆)δ: 0.62(3H, d, J=6.4 Hz), 0.78(3H, d, J=6.4 Hz), 1.55-1.69(3H, m), 1.93-1.99(1H, m), 2.97-3.08(2H, m), 4.39-4.46(3H, m), 4.50-4.59(2H, m), 4.77(1H, d, J=14.4 Hz), 7.03-7.09(1H, m), 7.20-7.28(1H, m), 7.36-7.43(1H, m), 8.59(1H, s), 10.39(1H, s), 12.56(1H, s).

EXAMPLE F-48 1-Cyclopropylmethyl-5-hydroxy-6,10-dioxo1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

melting point: 169-171° C.

NMR (DMSO-d₆)δ: 0.00-0.10(2H, m), 0.42-0.44(2H, m), 0.77-0.81(1H, m), 1.59-1.74(2H, m), 2.27-2.32(1H, m), 2.62-2.72(1H, m), 3.05-3.12(1H, m), 4.30-4.58(5H, 4.69(1H, d, J=14.8 Hz), 7.03-7.11(1H, m), 7.22-7.26(1H, m), 7.37-7.40(1H, m), 8.62(1H, s), 10.40(1H, t, J=6.0 Hz), 12.57(1H, s).

EXAMPLE F-49 1-Furan-2-ylmethyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

melting point: 186-188° C.

NMR (DMSO-d₆)δ: 1.55-1.68(2H, m), 2.55-2.64(1H, m), 2.88-2.99(2H, m), 3.80(2H, dd, J=15.6, 34.8 Hz), 4.36-4.56(5H, m), 4.97(1H, d, J=16.0 Hz), 6.39(2H, s), 7.05-7.08(1H, m), 7.21-7.26(1H, m), 7.37-7.44(1H, m), 7.58(1H, s), 8.64(1H, s), 10.38(1H, t, J=5.6 Hz), 12.53(1H, s).

EXAMPLE F-50 5-Hydroxy-6,10-dioxo-1-thiazol-2-ylinethyl-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

melting point: 168-170° C.

NMR (DMSO-d₆)δ: 1.59-1.74(2H, m), 2.76-2.83(1H, m), 2.97-3.08(2H, m), 3,89(1H, d, (J=16.4 Hz, 4.36(1H, d, J=1.6.0 Hz), 4.44-4.55(4H, m), 4.69(1H, s), 4.89(1H, d, J=14.8 Hz), 7.03-7.09(1H, m), 7.20-7.27(1H, m), 7.34-7.41(1H, m), 7.54(1H, d, J=3.2 Hz), 7.68(1H, d, J=3.2 Hz), 8.34(1H, s), 10.35(1H, d, J=6.0 Hz), 12.50(1H, s).

EXAMPLE F-51 5-Hydroxy-6,10-dioxo-1-pyridin-2-ylmethyl-1,2,3,4,6,9,9a,10-octalaydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

melting point: 200-203° C.

NMR (DMSO-d₆)δ: 1.54-1.61(1H, m), 1.69-1.78(1H, m), 2.71-2.79(1H, m), 2.91-3.09 (2H, m), 3.72(1H, d, J=14.4 Hz), 4.07(1H, d, J=14.4 Hz), 4.44-4.54(4H, m), 4.70(1H, s), 4.82(1H, d, J=14.4 Hz), 7.04-7,10(1H, m), 7.21-7.42(4H, m), 7.74-7.80(1H, m), 8.17(1H, s), 8.47-8.49(1H, m), 10.35(1H, d, J=6.0 Hz), 12.57(1H, s).

EXAMPLE F-52 1-Hydroxy-6-methyl-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalene-3-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 230-231° C.

NMR (DMSO-d₆)δ: 1.47-1.53(1H, m), 1.62-1.78(3H, m), 2.29(3H, s), 2.77-2.81 (2H, m), 4.05-4.10(1H, 4.35-4.4.0(1H, m), 4.54-4.64(3H, m), 4.70(1H s,), 7.18-7.22(1H, m), 7.30-7.34(1H, m), 7.47-7.52(1H, m), 8.49(1H, s), 10.47(1H, d, J=6.0 Hz), 12.44(1H, s).

EXAMPLE F-53 1-Hydroxy-6-isobutyl-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10atriaza-cyclohepta[b]naphthalene-3-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 215-216° C.

NMR (DMSO-d₆)δ: 0.83(6H, dd, J=6.8, 13.6 Hz), 1.45-1.80(5H, m), 2.36-2.41(1H, m), 2.77-2.93(2H, m), 3.17-3.24(1H, m), 4.02-4.09(1H, m), 4.32-4.40(2H, m), 4.61(2H, d, J=5.6 Hz), 4.82-4.84(1H, m), 7.18-7.22(1H, m), 7.30-7.33(1H, m), 7.48-7.51(1H, m), 8.47(1H, s), 10.48(1H, t, J=6.0 Hz), 12.55(1H, s).

EXAMPLE F-54 6-Cyclopropylmethyl-1-hydroxy-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalenc-3-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 212° C.

NMR (DMSO-d₆)δ: 0.00-0.10(2H, m), 0.40-45(2H, m), 0.80-0.87(1H, m), 1.45-1.77(3H, m), 2.64-2.69(1H, m), 2.85-2.95(2H, 3.13-3.20(1H, m), 4.03-4,09(1H, m), 4.36-4.40(2H, m), 4.59(2H, d, J=5.6 Hz), 4.84-4.86(1H, m), 7.16-7.20(1H, m), 7.28-7.32(1H, m), 7.46-7.50(1H, m), 8.45(1H, s), 10.46(1H, t, J=6.0 Hz), 12.50(1H, s).

EXAMPLE F-55 6-Furan-2-ylmethyl-1-hydroxy-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6, 10a-triaza-cyclohepta[b]naphthalene-3-carboxylic acid 3-chloro-2-fluoro-benzylamide

Melting point: 189-190° C.

NMR (DMSO-d₆)δ: 1.48-1.63(3H, m), 1.70-1.77(1H, m), 2.79-2.83(2H, m), 3.90(2H, dd, J=14,8, 39.6 Hz), 4.05-4.11(1H, m), 4.40-4.51(2H, m), 4.61(2H, d, J=5.6 Hz), 4.89-4.91(1H, m), 6.30-6.33(1H, m), .,38-6.40(1H, m), 7.18-7.22(1H, m), 7.30-7.34(1H, m), 7.48-7.53(1H, m), 7.57(1H, s), 8.45(1H, s), 10.45(1H, t, J=6.0 Hz), 12.44(1H, s).

EXAMPLE F-56 1-Hydroxy-6-methyl-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalene-3-carboxylic acid 2,4-difluoro-benzylamide

melting point: 241° C.

NMR (DMSO-d₆)δ: 1.47-1.53(1H, m), 1.62-1.78(3H, m), 2.29(3H, s), 2.77-2.81 (2H, m), 4.05-4.10(1H, m), 4.35-4.40(1H, m), 4.53-4,61(3H, m), 4.69(1H, s),'7.03-7.08(1H, m), 7.20-7.27(1H, m), 7.37-7.43(1H, m), 8.49(1H s,), 10.42(1H, d, J=6.0 Hz), 12.43(1H, s).

EXAMPLE F-57 1-Hydroxy-6-isobutyl-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalenc-3-carboxylic acid 2,4-difluoro-benzylamide

melting point: 203° C.

NMR (DMSO-d₆)δ: 0.82(6H, dd, J=6.4, 13.2 Hz), 1.45-1.80(5H, m), 2.36-2.42(1H, m), 2.77-2.93(2H, m), 3.15-3.23(1H, m), 4.02-4.08(1H, m), 4.32-4.41(2H, m), 4.54(2H, d, J=5.6 Hz), 4.82-4.84(1H, m), 7.02-7.09(1H, m), 7.20-7.27(1H, m), 7.36-7.43(1H, m), 8.47(1H, s), 10.41(1H, t, J=6.0 Hz), 12.54(1H, s).

EXAMPLE F-58 6-Cyclopropylmethyl-1-hydroxy-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naphthalene-3-carboxylic acid 2,4-difluoro-benzylamide

melting point: 182-183° C.

NMR (DMSO-d₆)δ: 0.00-0.10(2H, m), 0.40-45(2H, m), 0.80-0.87(1H, m), 1.43-1.77(3H, m), 2.60-2.69(1H, m), 2.85-2.95(2H, m), 3.11-3.19(1H, m), 4.00-4.06(1H, m), 4.36-4.40(2H, m), 4.51(2H, d, J=5.6 Hz), 4.83-4.87(1H, m), 7.00-7.07(1H, m), 7.16-7.23(1H, m), 7.34-7.38(1H, m), 8.44(1H, s), 10.39(1H, t, J=6.0 Hz), 12.47(1H, s).

EXAMPLE F-59 6-Furan-2-ylmethyl-1-hydroxy-2,11-dioxo-2,5a,6,7,8,9,10,11-octahydro-5H-4a,6,10a-triaza-cyclohepta[b]naplithalene-3-carboxylic acid 2,4-difluoro-benzylamide

melting point 171-173° C.

NMR (DMSO-d₆)δ: 1.47-1.64(3H, m), 1.70-1.77(1H, 2.79-2.83(2H, 3.90(2H, dd, J=15.6, 39.6 Hz), 4.05-4.11(1H, m), 4.41-4.57(4H, m), 4.90-4.92(1H, 6.30-6.33(1H, m), 6.38-6.40(1H, m), 7.03-7.09(1H, m), 7.20-7.27(1H, m), 7.37-7.45(1H, m), 7.57(1H, s), 8.44(1H, s), 10,41(1H, t, J=6.0 Hz), 12.43(1H, s).

EXAMPLE F-60 5-Hydroxy-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa.-4a,8a-diaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 276° C.

NMR. (DMSOd₆)δ: 1.60-1.68(1H, m), 1.77-1.84(1H, m), 3.85-3.93 (1H, m), 4.03-4.07(1H, m), 4.43-4.62(5H, m), 5.28(1H s,), 7.17-7.22(1H, m), 7.29-7.34(1H, m), 7.47-7.52(3H, m), 8.49(1H, s), 10.41(1H, d, J=6.0 Hz), 12.48(1H, s).

EXAMPLE F-61 5-Hydroxy-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

melting point: 258° C.

NMR (DMSO-d₆)δ: 1.60-1.69(1H, 1.77-1.85(1H, m), 3.86-3.92 (1H, m), 4.04-4.08(1H, m), 4.43-4.55(5H, m), 5.28(1H s,), 7.03-7.09(1H, m), 7.21-7.27(1H, m), 7.36-7.43(1H, m), 8.50(1H, s), 10.35(1H, d, J=6.0 Hz), 12.47(1H, s).

EXAMPLE F-62 5-Hydroxy-1-(2-methoxy-ethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 3-chloro-2-fluoro-benzylamide

melting point: 193° C.

NMR (DMSO-d₆)δ: 1.53-1.73(2H, m), 2.51-2.58(1H, m), 2.71.-2.78(1H, 2.81-2.87 (1H, m), 2.95-3.08(2H, m), 3.17(3H, s), 4.40-4.52(3H, m), 4.62(1H, d, J=5.6 Hz), 4.78(1H, d, J=14.4 Hz), 7.18-7.22(1H, m), 7.30-7.34(1H, m), 7.47-7.52(1H, m), 8.55(1H, s), 10.45(1H, d, J=6.0 Hz), 12.59(1H, s).

EXAMPLE F-63 5-Hydroxy-1-(2-methoxy-ethyl)-6,10-dioxo1,2,3,4,6,9,9a,10-octahydro-1,4a,8atriaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

melting point: 166-168° C.

NMR (DMSO-d₆)δ: 1.55-1.72(2H, m), 2.51-2.58(1H, m), 2.70-2.77(1H, m), 2.80-2.87 (1H, m), 2.97-3.07(2H, m), 3.18(3H, s), 4.39-4.52(3H, m), 4.54(1H, d, J=5.2 Hz), 4.78(1H, d, J=13.6 Hz), 7.03-7.09(1H, m), 7.20-7.27(1H, m), 7.37-7.43(1H, m), 8.55(1H, s), 10.40(1H, d, J=6.0 Hz), 12.58(1H, s).

EXAMPLE F-64 5Hydroxy-1-(1H-imidazol-4-ylmethyl)-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro1,4a,8a-triazaanthracene-7-carboxylic acid 4-fluorobenzylamide

(DMSO-d₆)δ: 1.55-1.59(1H, m), 1.64-1.70(1H, m), 2.58-2.66(1H, m), 2.87-2.95(2H, m), 3.67(1H, d, J=15.2 Hz), 3.73(1H, d, J=15.2 Hz), 4.34(1H, s), 4.38-4.43(1H, m), 4.47-4.54(3H, m), 5.05(1H, d, J=14.0 Hz), 7.00(1H, s), 7.13-7.19(2H, m), 7.33-7.38(1H, m), 7.59(1H, s), 8.55(1H, s), 10.41(1H, t, J=5.6 Hz), 11.95(1H, br s), 12.59(1H, s).

EXAMPLE H-1 1-Acetyl -5-hydroxy-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1H-1,3a,8a-triaza-cyclopenta[b]naphtbalene-7-carboxylic acid 4-fluorobenzylamide

-   1) To a solution of a compound 48 (120 mg, 0.26 mmol) in methylene     chloride (1.2 ml) were added triethylamine (43 μl, 0.31 mmol),     acetic anhydride (20 μl, 0.31 mmol), and 4-dimethylaminopyridine     (cat.) at room temperature, and the mixture was stirred for 30     minutes. Further, triethylamine (18 μl, 0.13 mmol) and acetic     anhydride (12 μl, 0.13 mmol) were added, and the mixture was stirred     for 4 hours, 2N hydrochloric acid was added, this was extracted with     chloroform, and the organic layer was washed with water, dried with     sodium sulfate, and concentrated under reduced pressure. Diisopropyl     ether was added to crystallize the material, which was filtered to     obtain 53 (112 mg) as a pale orange crystal at a yield of 86%. -   2) An Example compound H-1 (71 mg) was obtained at a yield of 82%     from a compound 53 (106 mg), according to the method of Example B-1     17),

melting point 290° C.

NMR (DMSO-d₆)δ: 2.08(3H, s), 3.44-4.21(5H, m), 4.51(2H, d, 5.7 Hz), 4.93(1H, m), 5.46-5.62(1H, m), 7.15(2H, t, 9.0 Hz), 7.34(2H, m), 8.49(1H, s), 10.40(1H, t, 5.7 Hz), 11.48(1H, s).

An Example compound H-2 was synthesized according to the same manner as that of Example H-1.

EXAMPLE H-2 1-Acetyl-5-hydroxy-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene7-carboxylic acid 4-fluoro-benzylamide

melting point: 290° C.

NMR (DMSO-d₆)δ: 1.95(2H, m), 2.14(3H, s), 2.85(2H, m), 4.45(4H, m), 4.51(2H, d, 5.7 Hz), 5.99(1H, s), 7.15(2H, t, 9.0 Hz), 7.34(2H, m), 8.37(1H, s), 10.46(1H, s), 12.28(1H, s).

EXAMPLE I-1 5-Hydroxy-1-methanesulfonyl-4,6-dioxo-2,3,4,6,9,9a-hexahydro-1H-1,3a,8a-triaza-cyclopenta[b]naphthalene-7-carboxylic acid 4-fluoro-benzylamide

-   1) To a solution of a compound 48 (140 mg, 0.30 mmol) in pyridine     (1.4 ml) were added methanesulfonyl chloride (28 μl, 0.36 mmol), and     4-dimethylaminopyridine (cat.) at room temperature, and the mixture     was stirred for 3 hours. After 2N hydrochloric acid was added, this     was extracted with ethyl acetate, and the organic layer was washed     with water, dried with sodium sulfate, and concentrated under     reduced pressure, Diisopropylether was added to crystallize the     material, which was filtered to obtain 54 (127 mg) as a pale orange     crystal at a yield of 78%. -   2) According to the method of Example B-1 17), an Example compound     I-1 (21 mg) was obtained at a yield of 21% from a compound 54 (123     mg).

melting point: 260° C.

NMR (DMSO-d₆)δ: 3.16(3H, s), 3.30-4.15(5H, m), 4.45(2H, d, 5.7 Hz), 4.27(2H, m), 5.36(1H, m), 7.14(2H, t, 8.7 Hz), 7.33(2H, m), 8.22(1H, s), 10.53(1H, s).

According to the same manner as that of Example I-1, an Example compound I-2 was synthesized.

EXAMPLE I-2 5-Hydroxy-1-methanesulfonyl-6,10-dioxo-1,2,3,4,6,9,9a,10-octahydro-1,4a,8a-triaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

melting point: 257-259° C.

NMR (DMSO-d₆)δ: 1.80-1.96(2H, m), 3.02-3.58(2H, m), 3.16(3H, s), 4.76(2H, m), 5.56(1H, s), 7.16(2H, t, 9.0 Hz), 7.35(2H, m), 8.36(1H, s), 10.39(1H, s).

EXAMPLE L-1 5,9-Dihydroxy-6,10-dioxo-3,4,6,9,9a,10-hexahydro-1H-2-oxa-4a,8a-dia.zaanthracene-7-carboxylic acid 4-fluoro-benzylamide

-   1) According to the method of synthesizing a compound 66, a compound     62 (278 mg, 57%) was obtained from a compotind 13 (357 mg). -   2) According to the method of synthesizing a compound 57, a compound     63 (202 mg, 79%) was obtained from a compound 62 (278 mg). -   3) To a solution of a compound 63 (200 mg, 0.403 mmol) in chloroform     (2 ml) were added dimethyl sulfoxide (286 μl, 4.03 mmol), and     triethylamine (337 μl, 2.42 mmol), the mixture was stirred for 10     minutes under ice-cooling, a sulfur trioxide-pyridine complex (321     mg, 2.02 mmol) was added, and the mixture was stirred at room     temperature for 2 hours. To the reaction solution was added water (3     ml), and chloroform was distilled off under reduced pressure,     followed by extraction with ethyl acetate, The organic layer was     washed with water, dried with anhydrous sodium sulfate, and the     solvent was distilled off under reduced pressure. The crystalline     residue was washed with ethyl acetate to obtain a compound 64 (60     mg) at a yield of 30%. -   4) Using a compound 64, and according to the method of synthesizing     Example A-1, an Example compound L-1 was synthesized.

NMR (DMSO-d₆)δ: 2.98-3.10(1H, m), 3.38-3.60(2H, m), 3.80-4.20(5H, 4.40-4.55(2H, m), 5.48(1H, brs), 5.85(1H, s), 7.15(2H, t, J=8.4 Hz), 7.33-7.37(2H, m), 8.45(1H, s), 8.60(1H, s), 10.27-10.42(1H, m), 12.61(1H, brs).

EXAMPLE M-1 1-Hydroxy-2,10-dioxo-2,4b,5,6,7,8,9,10-octahydro-4a,9a-diaza-benzo[a]azulene-3-carboxylic acid 4-fluoro-benzylamide

-   1) According to the method of synthesizing a compound 21, a compound     65 (207 mg) was obtained at a yield of 24% from a compound 13 (250     mg). -   2) According to the method of synthesizing a compound 64, a compound     66 (313 mg, 67%) was obtained from a compound 65 (470 mg). -   3) After trifluoroacetic acid (10 ml) was added to a compound 66     (100 mg, 0.020 mmol), the mixture was stirred at 75° C. for 4 hours.     The solvent was distilled off under reduced pressure, and, this was     diluted with chloroform, and added to ice water. This was washed     with an aqueous saturated sodium bicarbonate solution, a 10% aqueous     citric acid solution, and water, and dried with anhydrous sodium     sulfate, and the solvent was distilled off under reduced pressure.     The residue was subjected to silica gel column chromatography, and     fractions eluted with chloroform-methanol were concentrated under     reduced pressure, and recrystallized with ethyl acetate-diisopropyl     ether to obtain an Example compound M-1 (23 mg, 16%).

melting point 281-283° C.

NMR (DMSO-d₆)δ: 1.43-1.52(2H, m), 1.62-1.83(3H, m), 2.04-2.18(1H, m), 2.23-2.35(1H, m), 4.08-4.16(1H, m), 4.48-4.53(2H, m), 5.58-5.61(1H, m), 7.11-7.20(2H, m), 7.30-7.38(2H, m), 8.29(1H, s), 10.30-10.36(1H, m), 12.78(1H, brs).

EXAMPLE X-1 (R)-6-Hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahyrdro-1H-pyrido[1,2-a]pyrrolo[1,2-d]pyrazine-8-carboxylic acid 4-fluoro-benzylamide

-   1) Selenium dioxide (666 mg, 6.0 mmol) was added to the solution of     compound 2 (216 mg, 1.0 mmol) in bromobenzene (2 ml)). Then the     mixture was heated up to 1.60° C., and stirred for 16 h. After     celite filtration the solvent was evaporate. The precipitate was     purified by silicagel column chromatography, and fractions eluting     With n-hexan/EtOAc were concentrated under reduced pressure to     obtain compound 100 (164 mg, 71%) as a yellow oil.

1H-NMR (CDCl₃)δ: 5.52(1H, s), 6.50(1.14, d, J=6.0 Hz), 7.36(5H, m), 7.74(1H, d, J=6.3 Hz), 9.88(1H, s).

-   2) Sulfamic acid (1.50 g, 15.4 mmol) and NaClO₂ (1.05 g, 11.6 mmol)     was added to the solution of compound 100 (2.54 g, 11.0 mmol) in     acetone (20 ml)) and water (30 ml)). Then the mixture was stirred     for 3 h. The solvent was evaporated under reduced pressure to obtain     compound 101 (2.18 mg, 80%) as a white solid.

1H-NMR (DMSO-d₆)δ: 5.11(2H, s), 6.55(1H, d, J=5.4 Hz), 7.32-7.46(5H, m), 8.21(1H, d, J=5.7 Hz).

-   3) (R)-2-N-BOC-aminomethyl pyrrolidine (391 mg, 1.95 mmol) was added     to the solution of compound 101 (400 mg, 1.62 mmol).

1-(3-dimethylaminopropyl)3-ethylcarbodiimide hydrochloride (373 mg, 1.95 mmol), and 1-hydroxybenzotriazole (219 mg, 1.62 mmol) in THF (6 ml)). After sttiring for 16 h NaHCO₃ aqueous solution was added to the mixture. The mixture was extracted with EtOAc, which was washed with NH₄Cl aqueous solution and brine. The organic phase was dried. over MgSO₄. After a filtration the solvent was removed under reduced pressure to obtain compound 102 (694 mg, 100%) as a white solid.

1H-NMR (CDCl₃)δ: 1.46(9H, s), 1.56-2.14(4H, m), 3.29(4H, m), 4.18(1H, m), 5.24(1H, s), 5.27(1H, s), 6.46(1H, d, J=5.7 Hz), 7.35(5H, m), 7.69(1H, d, J=5.7 Hz).

-   4) The solution of compound 102 (694 mg, 1.95 mmol) in HCl/EtOAc (4     mol/l, 8 ml)) was stirred for 30 min. The soluvant was removed under     reduced pressur, diluted with EtOH (16 ml)) then. A saturated NaHCO₃     aqueous solution was added to the solution to control pH at 9. The     mixture was strred at 50° C. for 2 h, then diluted with water. The     mixture was extracted with CHCl₃, washed with brine, and dried. over     MgSO₄. The solvent was removed under reduced pressure to obtain     compound 103 (413 mg, 68%) as a yellow solid,

1H-NMR (CDCl₃)δ: 1.54-2.22(4H, m), 3.60(2H, m), 3.80(1H, t, J=12.0 Hz), 4.18(1H, d, J=12.0 Hz), 5.15(1H, d, J=9.9 Hz), 5.35(1H, d, J=9.9 Hz), 6.71(1H, d, J=5.4 Hz), 7.33(3H, m), 7.50(1H, d, J=5.1 Hz), 7.63(2H, d, J=7.2 Hz).

-   5) NaOAc (118 mg, 1.44 mmol) and bromine (0.234 ml, 2.62 mmol) were     added to the solution of compound 103 (408 mg, 1.31 mmol) in acetic     acid (8 ml)), stirred for 30 min then. An aqueous solution of NaOH     (2M) was added to the mixture, and extracted with CH₂Cl₂, washed     with brine, and dried over Na₂SO₄. The solvent was removed under     reduced pressure to give compound 104 (390 mg, 77%) as a white     solid.

1H-NMR (CDCl₂)δ: 1.55-2.19(4H, m), 3.55-4.02(5H, m), 5.12(1H, d, J=9.6 Hz), 5.35(1H, d, J=9.9 Hz), 7.29-7.38(3H, m), 7.61(1H, s), 7.67(2H, d, J=6.6 Hz).

-   6) Tetrakis triphenylphosphine paradium (0) (77 mg, 0.067 mmol) and     N,N-diisopropylethylamine (0.29 ml, 1.67 mmol) were added to the     solution of compound 104 (1.30mg, 0.334 mmol) in DMSO (2.6 ml)). the     mixture was stirred under CO atmosphere for 2 h at 80° C. The     reaction mixture was diluted with a saturated NH₄Cl aqueous     solution, extracted with EtOAc then. And the organic phase was     washed with brine, and dried over Na₂SO₄. The precipitate was     purified by silicagel column chromatography, and fractions eluting     witIrMeOH/EtOAc were concentrated under reduced pressure to obtain     compound 105 (115 mg, 75%) as a white oil.

1H-NMR (CDCl₃)δ: 1.56-2.33(4H, m), 3.66(2H, m), 3.90(2H, m), 4.19(1H, s), 4.66(2H, m), 5.20(1H, d, J=9.9 Hz), 5.37(1H, d, J=9.9 Hz), 7.00(2H, t, J=8.7 Hz), 7.33(5H, m), 7.61(2H, m), 8.39(1H, m), 10.50(1H, s).

-   7) A mixture of compound 105 (11.1 mg, 0.241 mmol) and     paradium-carhon (10%, 22 mg) in THF (8 ml)) and MeOH (2 ml) was     stirred under hydrogen atmosphere for 3 h. After celite filteration     the solvent was removed under reduced pressure to give the example     X-1 (57 mg, 64%) as a white solid.

Melting point: 274° C.

1H-NMR (DMSO-d₆)δ:1,56-2.25(4H, m), 3.48-3.65(2H, m), 4,01(2H, m), 4.51(2H, d, J=5.7 Hz), 4.71(1H, d, J=9.9 Hz), 7.14(2H, t, J=9.0 Hz), 7.33(2H, dd, J=5.7, 8.7 Hz), 8.41(1H, s), 10.44(1H, t, J=6.0 Hz), 12.18(1H, s).

The following compounds were synthesized using the similar method.

EXAMPLE X-2 (R)-6-Hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro-1H-pyrido[1,2-a]pyrrolo[1,2-d]pyrazine-8-carboxylic acid 2,4-difluoro-benzylamide

Melting point: 300° C.

1H-NMR (DMSO-d₆)δ: 1.03-2.20(4H, m), 3.39-3.66(2H, m), 4.02(2H, m), 4.54(2H, d, J=6.0 Hz), 4.71(1H, d, J=9.9 Hz), 7.06(1H, m), 7.23(1H, 7.38(1H, m), 8.41(1H, s), 10.43(1H, t, J=6.0 Hz), 12.19(1H, s).

EXAMPLE X-3 (R)-6-Hydroxy5,7-dioxo-2,3,5,7,11,11a-hexahydro-1H-pyrido[1,2-a]pyrrolo[1,2-d]pyrazine-8-carboxylic acid 3-chloro-2-fluoro-benzylamide

Melting point: 304° C.

1H-NMR (DMSO-d₆)δ: 3.44-3.66(2H, m), 4.01(2H, m), 4.61(2H, d, J=5.4 Hz), 1.70(1H, d, J=9.0 Hz), 7.20(1H, m), 7.31(1H, m), 7.49(1H, m), 8.41(1H s,), 10.49(1H, t, J=5.7 Hz), 12.20(1H, s).

EXAMPLE X-4 1-Hydroxy-2,9-dioxo-2,5,6,7,8,9,10,10a-octahydro-4a,8a-diaza-anthracene-3-carboxylic acid 4-fluoro-benzylamide

Melting point: 259° C.

1HNMR (DMSO-d₆)δ:1.33-1.79(6H, m), 2.51(1H, m), 3.88(1H, m), 4.12(1H, dd, J=9.3, 14.1 Hz), 4.38(1H, d, J=12.9 Hz), 4.53(3H, m), 7.16(2H, t, J=9.0 Hz), 7.34(2H, dd, J=5.7, 8.7 Hz), 8.39(1H, s), 10.44(1H, t, J=6.3 Hz), 12.84(1H, s).

According to the same manner as that of Example C-21, the following Example compounds Y-1 to Y-18 were synthesized.

EXAMPLE Y-1 (3S,9aS)-5-Hydroxy-3-methyl-6,10dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide EXAMPLE Y-9 (3R,9aR)-5-Hydroxy-3-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diazaanthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (CDCl₃)δ: 0.90(3H, d, J=6.9 Hz), 2.00-2.10(1H, m), 2.70(1H, dd, J=11.6, 13.4 Hz), 3.41(1H, dd, J=11.2, 12.9 Hz), 4.05-6.45(2H, m), 4.30-4.38(1H, dd, J=4.0, 14.1 Hz), 4.63(2H, d, J=5.9 Hz), 6.65-4.75(1H, m), 4.98(1H, t, J=3.7 Hz), 6.80-6.84(2H, m), 7.32-7.40(1H, m), 8.31(1H, s), 10.38(1H, brs), 12.37(1H, s).

EXAMPLE Y-2 (4S,9aR)-5Hydroxy-4-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluorobenzylamide EXAMPLE Y-3 (4R,9aS)-5-Hydroxy-4-methyl-6,10-dioxo3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (CDCl₃)δ: 1.42(3H, d, J=7.0 Hz), 1.56(1H, dd, J=2,0, 14.0 Hz), 2.19-2.30(1H, m), 4.02(1H, d, J=2.2 Hz), 4.05(1H, t, J=2.3 Hz), 4.12(1H, dd, J=6.0, 13.6 Hz), 4.27(1H, dd, J=4.2, 13.4 Hz), 4.64(2H, d, J=5.9 Hz), 4.95-5.05(1H, m), 5.26(2H, d, J=4.1, 5.8 Hz), 6.75-6.85(2H, m), 7.30-7.40(1H, m), 8.30(1H, s), 10.38(1H, brs), 12.45(1H, s).

EXAMPLE Y-4 (2R,9aR)-5-Hydroxy-2-methoxymethyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4-a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide EXAMPLE Y8 (2S,9 aS) -5-Hydroxy-2 -methoxymethyl-6,10-dioxo 3,4,6,9,9a,10-hexahydro-2H-1 -oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (CDCl₃)δ: 1.60-1.80(2H, m), 3.09-3.21(1H, m), 3.37(3H, s), 3.35-3.50(2H, m), 4.00-4.11(1H, m), 4.24(1H, d, J=13.1 Hz), 4.36(1H, d, J=10.1 Hz), 4.64(1H, d, J=5.9 Hz), 4.70-4.80(1H, m), 5.12(1H s,), 6.75-6.85(2H, m), 7.30-7.40(114, m), 8.30(1H, s), 10.38(1H, brs), 12.33(1H, brs).

EXAMPLE Y-5 (5aR,6a8,10aR)-1-Hydroxy-2,12-clioxo-2,5,5a,7,8,9,10,10a,11,12-decahydro-6aH-6-oxa-4a,11a-diaza-naphthacene-3-carboxylic acid 2,4-difluoro-benzylamide [racemate]

1H-NMR (DMSO-d₆)δ: 1.00-1.85(9H, m), 2.90(1H, t, J=4.2 Hz), 4.36(1H, dd, J=4.2, 12.9 Hz), 4.44-4.57(4H, 5.32(1H, t, J=3.9 Hz), 7.03-7.09(1H, m), 7.20-7.27(1H, m), 7.35-7.43(1H, m), 8.49(1H, s), 10.34(1H, brs).

EXAMPLE Y-6 (2S,9aR)-2-Ethyl-5-hydroxy-6,10-dioxo3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide EXAMPLE Y-7 (2R,9aS)-2-Ethyl-5-hydroxy-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (DMSO-d₆)δ: 0.87(3H, d, 1.40-1.51(3H, m), 1.75(1H, d, J=10.8 Hz), 3.22(1H, t, J=10.2 Hz), 3.73-3.78(1H, m), 4.41-4.57(4H, m), 5.29(1H, s), 7.03-7.07(1H, m), 7.21-7.26(1H, m), 7.37-7.42(1H, 8.50(1H, s), 10.34(1H, brs), 12.48(1H, s).

EXAMPLE Y-10 (2S,9aS)-5-Hydroxy-6,10-dioxo-2-phenyl-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (CDCl₃)δ: 1.70-1.82(1H, m), 1.98(1H, d, J=9.6 Hz), 3.49(1H, t, J=9.6 Hz), 4.54-4.68(5H, m), 4.98(1H, d, J=8.7 Hz), 5.51(1H, s), 7.04-7.08(1H, m), 7.21-7.42(7H, m), 8.50(1H s,), 10.38(1H, s), 12.45(1H, s).

EXAMPLE Y-11 (2S,9aS)-5-Hydroxy-2-isopropyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide EXAMPLE Y-12 (2R,9aR)-5-Hydroxy-2-isopropyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (DMSO-d6)δ: 0.86(6H, dd, J=4.8, 13.5 Hz), 1.41-1.49(1H, m), 1.57-1.69(1H, m), 1.72-1.78(1H, m), 3.20(1H, t, J=8.4 Hz), 3.52-3.59(1H, m), 4.41-4.46(5H, m), 5.29(1H, s), 7.01-7.08(1H, m), 7.21-7.26(1H, m), 7.37-7.43(1H, m), 8.50(1H, s), 10.35(1H, brs), 12.48(1H, s).

EXAMPLE Y-13 (3S,9aS)-5-Hydroxy-3-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide EXAMPLE Y-14 (3R,9aR)-5-Hydroxy-3-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

1H-NMR (DMSO-d6)δ: 0.81(3H,d, J=6.6 Hz), 1.84-1.93(1H, m), 2.86(1H, t, J=12.5 Hz), 3.48(1H, t, J=11.1 Hz), 3.97-4.03(1H, m), 4.41-4.60(3H, m), 4.52(2H, d, J=5.9 Hz), 5.20(1H, t, J=3.8 Hz), 7.12-7.20(2H, m), 7.32-7.38(2H, m), 8.52(1H, s), 10.36(1H, t, J=5.9 Hz), 12.45(1H, s).

EXAMPLE Y-15 (2R,9aS)-5-Hydroxy-2-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide EXAMPLE Y-16 (2S,9aR)-5-Hydroxy-2-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 2,4-difluoro-benzylamide

1H-NMR (DMSO-d6)δ: 1.14(3H, d, J=6.0 Hz), 1.38(1H, m), 1.75(1H, d, J=13.8 Hz), 3.18-3.29(1H, m), 3.95-4.06(1H, m), 4.42-4.58(3H, m), 4.54(2H, d, J=5.7 Hz), 5.30(1H, t, J=3.9 Hz), 7.03-7.10(1H, m), 7.20-7.29(1H, m), 7.35-7.44(1H, m), 8.50(1H, s), 10.35(1H, t, J=5.7 Hz), 12.48(1H, s).

EXAMPLE Y-17 (2S,9aR)-5-Hydroxy-2-methyl-6,10-dioxo-3,4,6,9,9a,10-hexahydro-2H-3-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide EXAMPLE Y-18 (2R,9aS)-5-Hydroxy-2-methyl-6,10-dioxo-3,4,6,9,9 a,10-hexahydro-2H-1-oxa-4a,8a-diaza-anthracene-7-carboxylic acid 4-fluoro-benzylamide

1H-NMR (DMSO-d₆)δ: 1.15(3H, d, J=6.0 Hz), 1.35-1.50(1H, m), 1.75(1H, d, J=12.9 Hz.), 3.23(1H, td, J=13.0, 3.95-4.03(1H, m),4.41-4.59(8H, 4.52(2H, d, J=6.0 Hz), 5.30(1H, t, J=3.9 Hz), 7.12-7.19(2H, m), 7.32-7.38(2H, m), 8.52(1H, s), 10.36(1H, t, J=6.0 Hz), 12.48(1H, s).

Corresponding amino-alcohol derivatives used in syntheses of Y-1 to Y-18 were prepared as optically pure version using methods similar to those described in the following reports.

-   3-Amino-2-methyl-propan-1-ol, and 4-Amino-butan-2-ol were prepared     according to the method of Russell A. Barrow (J. Am, Chem. Soc.     1995, 117, 2479-2490), -   3-Amino-butan-1-el were prepared according to the method of P. Besse     (Tetrahedron Asymmetry 10(1999) 2213-2224.). -   1-Amino-pentan-3-ol, 1-Amino-4-methyl-pentan-3-ol,     4-Amino-1-methoxy-butan-2-ol, and 3-Amino-1-phenyl-propan-1-ol were     prepared. according to the method described in the following     literatures, U.S. Pat. Appl. Publ., 2004133029, 8 Jul. 2004, PCT     Int, Appl., 2002012173, 14 Feb. 2002.

All examples below consist of >95% ee and >6:1 diastereomeric purity unless indicated otherwise. The compounds shown in table ZZ consist of mixtures of diastereoniers at the depicted stereocenter in ratios of 1:1 to >10:1, Stereocenters that were formed during the process below have been assigned using NMR techniques well know in the art (1D and 2D method) and/or using vibrational circular dichroism techniques. Stereochemical assignment determinatons were performed on representative examples and closely related compounds were assigned by analogy in sonic cases. The schemes below are meant to be general guidance to how examples were synthesized. It will be possible that one skilled in the art may rearrange the order of steps or change substituents to apply the method described below and in the examples to construct compounds of the general formula. Additional methods known to those skilled in the art or commonly present in the literature may also be applied to perform similar transformations and arriving at the same compounds of the general formula or amino alcohol and diamino precursors.

EXAMPLE Z-1 (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide sodium salt

a)

(3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-3-methyl-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexalaydro[1,3]oxazolo[3,2a]pyrido[1,2-d]pyrazine-8-carboxamide. To a solution of 16a (409 mg, 0.87 mmol) in dichloroethane (20 mL) was added (2R)-2-amino-1-propanol (0.14 mL, 1.74 mmol) and 10 drops of glacial acetic acid. The resultant solution was heated at reflux for 2 h. Upon cooling, Celite was added to the mixture and the solvents removed in vacuo and the material was purified via silica gel chromatography (2% CH₃OH/CH₂Cl₂ gradient elution) to give (3R,11aS)-N-[(2,4-difluorophenyl)methyl]-3-methyl-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide (396 mg, 92%) as a glass. ¹H NMR (CDCl₃) δ 10.38 (m, 1H), 8.42 (s, 1H), 7.54-7.53 (m, 2H), 7.37-7.24 (m, 4H), 6.83-6.76 (m, 2H), 5.40 (d, J=10.0 Hz, 1H), 5.22 (d, J=10.0 Hz, 1H), 5.16 (dd, J=9.6, 6.0 Hz, 1H), 4.62 (m, 2H), 4.41 (m, 1H), 4.33-4.30 (m, 2H), 3.84 (dd, J=12.0, 10.0 Hz, 1H), 3.63 (dd, J=3.4, 7.2 Hz, 1H), 1.37 (d, J=6.0 Hz, 3H); ES⁺MS: 496 (M+1).

b)

(3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide sodium salt. To a solution of (3R,11aS)-N-[(2,4-difluorophenyl)methyl]-3-methyl-5,7-dioxo-6[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (396 mg, 0.80 mmol) in methanol (30 mL) was added 10% Pd/C (25 mg). Hydrogen was bubbled through the reaction mixture via a balloon for 2 h. The resultant mixture was filtered through Celite with methanol and dichloromethane. The filtrate was concentrated in vacuo to give (3R,11aS)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexalaydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide as a pink tinted white solid (278 mg, 86%). ¹H NMR (CDCl₃) δ 11.47 (m, 1H), 10.29 (m, 1H), 8.32 (s, 1H), 7.36 (m, 1H), 6.82 (m, 2H), 5.31 (dd, J=9.6, 3.6 Hz, 1H), 4.65 (m, 2H), 4.47-4.38 (m, 3H), 3.93 (dd, J=12.0, 10.0 Hz, 1H), 3.75 (m, 1H), 1.49 (d, J=5.6 Hz, 3H); ES⁺ MS: 406 (M+1). The above material (278 mg, 0.66 mmol) was taken up in ethanol (10 mL) and treated with 1 N sodium hydroxide (aq) (0.66 mL, 0.66 mmol). The resulting suspension was stirred at room temperature for 30 min. Ether was added and the liquids were collected to provide the sodium salt of the title compound as a white powder (291 mg, 99%). ¹H NMR (DMSO-d₆) δ 10.68 (m, 1H), 7.90 (s, 1H), 7.35 (m, 1H), 7.20 (m, 1H), 7.01 (m, 1H), 5.20 (m, 1H), 4.58 (m, 1H), 4.49 (m, 2H), 4.22 (m, 2H), 3.74 (dd, J=11.2, 10.4 Hz, 1H), 3.58 (m, 1H), 1.25 (d, J=4.4 Hz, 3H).

EXAMPLE Z-2 (4aR,13aS)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide

a)

(4aR,13aS)-N-[(2,4-Difluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide. A solution of 16a (24 mg, 0.05 mmol), [(2S)-2-pyrrolidinylmethyl]amine (0.1 mL) and 2 drops of glacial acetic acid were heated under microwave conditions at 140° C. for 10 min. Upon cooling, Celite was added to the mixture and the solvents removed in vacuo and the matezial was purified via silica gel chromatography (2% CH_(a)OH/CH₂Cl₂ gradient elution) to give (4aR,13aS)-N-[(2,4-difluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazinc-8-carboxamide (19 mg, 71%) as a white solid. NMR (CDCl₃) δ 10.41 (m, 1H), 8.38 (s, 1H), 7.56 (m, 2H), 7.38-7.24 (m, 4 H), 6.80 (m, 2H), 5.38 (d, J=9.6 Hz, 1H), 5.10 (d, J=10.0 Hz, 1H), 4.62 (m, 2H), 4.40 (m, 1H), 4.25 (dd, J=12.0, 6.8 Hz, 1H), 4.10 (d, J=12.8 Hz, 1H), 3.83 (m, 1H), 3.71 (m, 1H), 3.14-3.04. (n, 2H), 2.78 (m, 1H), 2.11-1.58 (m, 4H); ES⁺ MS: 521 (M+1).

b)

(4aR,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide.

To a solution of (4aR,13aS)-N-[(2,4-difluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide (19 mg, 0.04 mmol) in methanol (8 mid) was added 10% Pd/C (10 mg). Hydrogen was bubbled through the reaction mixture via a balloon for 2 h. The resultant mixture was filtered through Celite with methanol and dichloromethane. The filtrate was concentrated in-vacuo to give the title compound (6 mg, 38%) as a white solid. ¹H NMR (CDCl₃) δ 11.78 (m, 1H), 10.36 (m, 1H), 8.31 (s, 1. H), 7.33 (m, 1H), 6.78 (m, 2H), 4.62 (m, 2H), 4.50 (m, 1H), 4.27-4.19 (m, 2H), 3.87-3.77 (m, 2H), 3.16-3.08 (m, 2H), 2.83 (m, 1H), 2.11-1.65 (m, 4.14): ES⁺ MS: 481 (M+1).

EXAMPLE Z-3 (3aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-8-hydroxy-7,9-dioxo-1,2,3,3a,4,53 9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino [1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide

-   a) N-BOC-(2S)-2-(Hydroxymethyl)-1-pyrrolidine. To a solution of     N-BOC-L-proline (4.17 g, 19.4 mmol) in THF (40 mL) at 0° C. was     added BH₈-THF (21.4 mL, 1 M in THF, 21.4 mmol) dropwise. The bath     was removed and the resultant solution stirred at room temperature     for 2 h. Methanol was added to quench the mixture and the solvents     were removed in vacuo. The residue was taken up in ethyl acetate and     washed with sodium bicarbonate and brine. The aqueous layers were     extracted twice with ethyl acetate, The combined organics were dried     over Na₂SO₄, filtered and concentrated to give     N-BOC-(2S)-2-(hydroxymethyl)-1-pyrrolidine (3.82 g, 98%) as a clear     oil. This material was used without further purification. ¹H NMR     (CDCl₃) δ 3.94 (m, 1H), 3.62 (dd, J=11.2, 3.2 Hz, 1H), 3.56 (dd,     J=10.8, 7.2 Hz, 1H), 3.44 (m, 1H), 3.29 (m, 1H), 2.62 (br, 1H), 1.98     (m, 1H), 1.85-1.72 (m, 2H), 1.58 (m, 1H). -   b)     N-BOC-(2S)-2-({[(4-Methylphenyl)sulfonyl]oxy}methyl)-1-pyrrolidine.     To a cold (0° C.) solution of     N-BOC-(2S)-2-(hydroxymethyl)-1-pyrrolidine (350 mg, 1.74 mmol) in     dichloromethane (20 mL) was added triethylamine (0.29 mL, 2.08     mmol), and toluenesulfonyl chloride (398 mg, 2.08 mmol).     N,N-demethylaminopyridine (70 mg) was added and the resultant     solution was allowed to warm to rt as the bath warmed and stirred     for 4 h. Water was added and the layers separated. The aqueous layer     was washed with sodium bicarbonate and then with brine, The combined     organics were dried over Na₂SO₄, filtered and concentrated followed     by flash chromatography purification to give     N-BOC-(2S)-2-({[(4-methylphenyl)sulfonyl]oxy}methyl)-1-pyrrolidine     (460 mg, 75%) as a clear oil, ¹H NMR exists as rotomers (CDCl₃) δ     7.77 (d, 2H), 7.33 (m, 2H), 4.08 (m, 1H), 3.97-3.88 (m, 1H),     3.35-3.25 (m, 2H), 2.43 (s, 3H), 1.95-1.79 (m, 4H), 1.40 and 1.35     (s, 9H rotomeric BOC t-butyl). -   c) N-BOC-(2S)-2-Cyano-1-pyrrolidine. A mixture of     N-BOC-(2S)-2-({[(4-methylphenyl)sulfonyl]oxy}methyl)-1-pyrrolidine     (460 mg, 1.29 mmol) and KCN (256 mg, 3.88 mmol) were heated at     90° C. in DMSO (10 mL) for 6.5 h. The mixture was cooled to room     temperature and EtOAc and water were added. The organics were washed     with water twice and then with brine. The aqueous layers were     extracted with EtOAc and the combined organics dried over Na₂SO₄,     filtered and concentrated followed by flash chromatography     purification to give N-BOC-(2S)-2-cyano-1-pyrrolidine (179 mg, 66%)     as an oil. ¹H NMR exists as rotomers (CDCl₃) δ 3.99 (m, 1H),     3.43-3.37 (m, 2H), 2.83-2.51 (m, 2H), 2.17-1.83 (m, 4 H), 1.46 and     1.44 (s, 9 H rotomeric BOC t-butyl). -   d) N-BOC-(2S)-2-(2-Aminoethyl)-1-pyrrolidine. A solution of     N-BOC-(2S)-2-cyano-1-pyrrolidine (179 mg, 0.85 mmol) in ethanol     saturated with anhydrous ammonia was treated with Raney-Ni (1 mL of     50% aq. Suspension) and 50 psi of H₂ overnight. The mixture was     filtered through Celite and the filtrate was concentrated in vacuo.     The residue was purified by flash chromatography (10% CH₃OH/CH₂Cl₂     with 1% NH₄OH gradient elution) through a short plug of silica gel     to give N-BOC-(2S)-2-(2-aminoethyl)-1-pyrrolidine (90 mg, 50%) as a     clear oil. ¹H NMR exists as rotomers (CDCl₃) δ 3.88-3.77 (m, 1H),     3.33-3.24 (m, 2H), 2.66 (m, 2H), 1.89-1.54 (m, 6H), 1.40 (s, 9H). -   e) {2-[(2S)-2-Pyrrolidinyl]ethyl)amine. A solution of     N-BOC-(2S)-2-(2-aminoethyl)-1-pyrrolidine (90 mg, 0.42 mmol) in THF     (6 mL) was treated with 4 N HCl (aq) (2 mL) and stirred at room     temperature for 3 h. The mixture was concentrated in vacuo to give     the title compound as its HCl salt. A portion of this material (40     mg) was dissolved in methanol and treated with solid supported     carbonate resin (MP-Carbonate, Argonaut Technologies) to freebase     the amines. After 30 minutes, the. solution was filtered through a     fritted tube and the solvents removed carefully in vacuo to give     {2-[(2S)-2-pyrrolidinyl]ethyl}amine (30 mg) as its free base. ¹H NMR     (CDCl₃) δ 3.06 (m, 1H), 2.94 (m, 1H), 2.83 (m, 1H), 2.79-2.69 (m,     2H), 1.90-1.56 (m, 6 H).     f)

(3aS,13a S)-N-[(2,4-Difluorophenyl)methyl]-7,9-dioxo-8-[(phenylmethyl)oxy]-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide

A solution of 16a (30 mg, 0.06 mmol), {2-[(2S)-2-pyrrolidinyl]ethyl}amine (30 mg, 0.26 mmol) and 2 drops of glacial acetic acid were heated under microwave conditions at 140° C. for 10 min. Upon cooling, Celite was added to the mixture and the solvents removed in vacuo and the material was purified via silica gel chromatography (2% CH₃OH/CH₂Cl₂ gradient elution) to give (3aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-7,9-dioxo-8-[(phenylmethyl)oxy]-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrolo [1,2-c]pyrimidine-10-carboxamide. (25 mg, 74%) as a film. ¹H NMR (CDCl₃) δ 10.44 (m, 1H), 8.32 (s, 1H), 7.59 (m, 2H), 7.38-7.24 (m, 4H), 6.80 (m, 2H), 5.28-5.22 (m, 2H), 4.67 (dd, J=13.6, 2.8 Hz, 1H), 4.62 (m, 2H), 4.26 (m, 1H), 4.11-4.03 (m, 2H), 2.91 (m, 1H), 2.81 (m, 1H), 2.37 (m, 1H), 2.24 (m, 1H), 1.92 (m, 1H), 1.82-1.76 (m, 3H), 1.52-1.38 (m, 2H); ES⁺ MS: 535 (M+1).

g)

(3aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-8-hydroxy-7,9-dioxo-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide

To a solution of (3aS,13aS)-N-[(2,4-difluorophenyl)methyl]-7,9-dioxo-8-[(phenylmethyl)oxy]1,2,3,3a,4,5,7,9,13,13a decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide (25 mg, 0.05 mmol) in methanol (8 mL) was added 10% Pd/C (10 mg). Hydrogen was bubbled through the reaction mixture via a balloon for 18 h. The resultant mixture was filtered through. Celite with methanol and dichloromethane. The filtrate was concentrated in vacua to give the title compound (14 mg, 67%) as a white solid. ¹H NMR (CDCl₃) δ 12.53 (br, 1H), 10.44 (s, 1H), 8.29 (s, 1H), 7.34 (m, 1H), 6.78 (m, 2H), 4.71-4.58 (m, 3H), 4.29-4.14 (m, 3H), 2.99 (m, 1H), 2.88 (m, 1H), 2.44 (m, 1H), 2.30 (m, 1H), 1.97-1.38 (m, 6H); ES⁺ MS: 445 (M+1).

EXAMPLE Z-4 (4aS,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′, 2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide sodium salt

-   a) [(2R)-2-Pyrrolidinylmethyl]amine. To a solution of     N-BOC-(2R)-2-(aminomethyl)-1-pyrrolidine (1.37 g, 6.85 mmol) in THF     (20 mL) was added 4 NHCl (aq) (8 mL). The resultant solution was     stirred at room temperature overnight. The solvents were removed in     vacua and the residue was treated with MP-carbonate resin in     methanol and dichloromethane. After 1 h, the resin was removed via     filtration through a fritted tube and the volatiles were removed     carefully in vacua to produce the free based amine (760 mg     crude >100%) as a oil. This material was used without further     purification, ¹H NMR (CDCl₃) δ 3.13 (m, 1H), 2.92 (m, 1H), 2.82-2.62     (m, 5H), 1.88-1.30 (m, 4H).     b)

(4aS,13aR))-N-[(2,4-Difluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide. In a similar manner as described in example 7.2 from 16a (435 mg, 0.93 mmol) and [(2R)-2-pyrrolidinylmethyl]amine (200 mg, 2.0 mmol) in 1,2-dichloroethane (20 mL) and 15 drops of glacial acetic acid was obtained (4aS,13aR)-N-[(2,4-difluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide (321 mg, 67%) as a white solid. ¹H NMR (CDCl₃) δ 10.41 (m, 1H), 8.35 (s, 1H), 7.56 (m, 2H), 7.55-7.24 (m, 4 H), 6.80 (m, 2H), 5.35 (d, J=10.0 Hz, 1H), 5.13 (d, J=10.0 Hz, 1H), 4.60 (m, 2H), 4.38 (dd, J=10.4, 3.2 Hz, 1H), 4.21 (dd, J=12.0, 6.8 Hz, 1H), 4.04 (dd, J=12.4, 2.8 Hz, 1H), 3.77 (apparent t, J=11.6 Hz, 1H), 3.68 (m, 1H), 3.11-3.00 (m, 2H), 2.75 (m, 1H), 2.08-1.84 (m, 8H), 1.65 (m, 1H); ES⁺ MS: 521 (M+1).

c)

(4aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octabydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-a]pyrazine-8-carboxamide

In a similar manner as described in example Z-2 from (4aS,13aR)-N-[(2,4-difluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octabydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide (518 mg, 0.99 mmol) and 10% Pd/C (35 mg) in methanol (40 mL) was obtained (4aS,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide (430 mg, 99%) as a white solid. ¹H NMR (CDCl₃) δ 11.73 (m, 1H), 10.36 (m, 1H), 8.32 (s, 1H), 7.35 (m, 1H), 6.79 (m, 2H), 4.64 (m, 2H), 4.54 (dd, J=10.8, 4.0 Hz, 1H), 4.28-4.19 (m, 2H), 3.90-3.79 (m, 2H), 3.18-3.10 (m, 2H), 2.84 (m, 1H), 2.14-1.92 (m, 3H), 1.72 (m, 1H).

d)

(4aS,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide sodium salt. In a similar manner as described in example Z-1 from (4aS,13aR)-N-[(2,4-Difluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide (430 mg, 1.0 mmol) and sodium hydroxide (1.0 mL, 1.0 M aq, 1.0 mmol) in 20 mL of ethanol was formed the corresponding sodium salt (425 mg, 94%) as a white solid. ¹H NMR (D₂O) δ 7.85 (s, 1H), 7.23 (m, 1H), 6.82 (m, 2H), (m, 3H), 4.28 (m, 1H), 3.95 (m, 1H), 3.84. (m, 1H), 3.62 (m, 1H), 3.16 (m, 1H), 2.89 (m, 1H), 2.84 (m, 1H), 1.90 (m, 2H), 1.73 (m, 1H), 1.60 (m, 1H). ES⁺ MS: 431 (M+1). EXAMPLE Z-5 (4aS,13aR)-N-[(4-Fluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2d]pyrazine-8-carboxamide.

The title compound was made in two steps using a similar process to that described in example Z-2. 16 (60 mg, 0.13 mmol) and [(2R)-2-pyrrolidinylmethyl]amine (100 mg, 1.0 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4aS,13aR)-N-[(4-fluorophenyl)methyl]-9,11-dioxo-10-[(phenylmethyl)oxy]-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2a]pyrazine-8 -carboxamide (60 mg, 91%). This material was hydrogenated in a second step as described in example Z-2 to give

(4aS,13aR)-N-[(4-fluorophenyl)methyl]-10-hydroxy-9,11-dioxo-2,3,4a,5,9,11,13,13a-octahydro-1H-pyrido[1,2-a]pyrrolo[1′,2′:3,4]imidazo[1,2-d]pyrazine-8-carboxamide (21 mg, 42%) as a white solid. NMR (CDCl₈) δ 11.72 (m, 1H), 1.37 (m, 1H), 8.33 (s, 1H), 7.29 (m, 2H), 6.97 (m, 2H), 4.57 (m, 2H), 4.52 (m, 1H), 4.24-4.19 (m, 2H), 3.87-3.76 (m, 2H), 3.14-3.07 (m, 2H), 2.82 (m, 1H), 2.11-1.89 (m, 3H), 1.68 (m, 1H); ES⁺ MS: 4.13 (M+1). EXAMPLE Z-6 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-8-(phenylmethyl)-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2, 16a (37 mg, 0.08 mmol) and (2S)-2-amino-3-phenyl-1-propanol (35 mg, 0.24 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-3-(phenylmethyl)-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (4.1 mg, 91%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-3-(phenylmethyl)-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide. (25 mg, 75%) as a white solid. ¹H NMR (CDCl₃) δ 11.47 (br, 1H), 10.28 (m, 1H), 8.35 (m, 1H), 7.37-7.26 (m, 4H), 7.18 (m, 2H), 6.79 (m, 2H), 5.03 (m, 1H), 4.64-4.61 (m, 3H), 4.40 (m, 1H), 4.23 (apparent t, J=7.2 Hz, 1H), 3.96 (dd, J=8.8, 6.4 Hz, 1H), 3.88 (apparent t, J=11.2 Hz, 1H), 3.37 (dd, J=13.6, 3.2 Hz, 1H), 2.99 (dd, J=13.2 8.8 Hz, 1H); ES⁺ MS: 482 (M+1).

EXAMPLE Z-7 (3a S,13aS)-N-[(4-Fluorophenyl)methyl]-8-hydroxy-7,9-dioxo-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrazino[1,2-c]pyrimidine-10-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16 (84 mg, 0.13 mmol) and {2-[(2S)-2-Pyrrolidinyl]ethyl}amine (150 mg, 1.3 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3aS,13aS)-N-[(4-fluorophenyl)methyl]-7,9-dioxo-8-[(phenylmethyl)oxy]-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide (86 mg, 90%). This material was hydrogenated in a second step as described in example Z-2 give

(3aS,13aS)-N-[(4-Fluorophenyl)methyl]-8-hydroxy-7,9-dioxo-1,2,3,3a,4,5,7,9,13,13a-decahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrrolo[1,2-c]pyrimidine-10-carboxamide. (63 mg, 88%) as a white solid. ¹H NMR (CDCl₃/CD₃OD) δ 10.45 (m, 1H), 8.23 (s, 1H), 7.35 (m, 2H), 6.94 (t, J=8.8 Hz, 2H), 4.63 (m, 1H), 4.58-4.48 (m, 2H), 4.33 (dd, J=13.6, 3.6 Hz, 1H), 4.21 (m, 1H), 4.11 (m, 1H), 2.98 (m, 1H), 2.85 (td, J=13.2, 3.2 Hz, 1H), 2.41 (m, 1H), 2.29 (m, 1H), 1.92 (m, 1H), 1.83-1.75 (m, 3H), 1.54-1.35 (m, 2H); ES⁺ MS: 427 (M+1).

EXAMPLE Z-8 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(1S)-1-methvlpropyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide sodium salt

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (417 mg, 0.89 mmol) and L-isoleucinol (259 mg, 2.21 mmol) were reacted in 1,2-dichloroethane (40 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-[(1S)-1-methylpropyl]-5,7 -dioxo-6-[(phenyl methyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (426 mg, 90%), This material was hydrogenated in a second step as described in example Z-1 to give (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(1S)-1-methylpropyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (376 mg, 99%) as a coarse white solid. ¹H NMR (CDCl₃) δ 11.43 (br, 1H), 10.27 (br, 1H), 8.32 (s, 1H), 7.33 (m, 1H), 6.79 (m, 2H), 5.26 (dd, J=9.6, 4.0 Hz, 1H), 4.62 (m, 2H), 4.42-4.35 (m, 2H), 4.19 (dd, J=8.8, 7.2 Hz, 1H), 4.01 (dd, J=8.8, 5.6 Hz, 1H), 3.86 (dd, J=12.0, 10.0 Hz, 1H), 2.27 (m, 1H), 1.40 (m, 1H), 1.15 (m, 1H), 0.97 (t, J=7.2 Hz, 3H), 0.91 (d, J=6.8 Hz, 3H); ES⁺ MS: 448 (M+1). This material (360 mg, 0.81 mmol) was treated with sodium hydroxide (0.81 mL, 1.0 M, 0.81 mmol) in ethanol (15 mL) as described in example Z-1 to provide its corresponding sodium salt (384 mg, 99%) as a white solid. ¹H NMR (DMSO-d₆) δ 10.82 (m, 1H), 7.80 (m, 1H), 7.33 (m, 1H), 7.18 (m, 1H), 7.00 (m, 1H), 5.14 (m, 1H), 4.47 (d, J=5.6 Hz, 2H), 4.31 (m, 1H), 4.18 (m, 1H), 3.96 (m, 1H), 3.84 (m, 1H), 3.71 (m, 1H), 3,40 (m, 1H), 1.88 (m, 1H), 1.36 (m, 1H), 1.04 (m, 1H), 0.85 (t, J=7.2 Hz, 3H), 0.80 (d, J=6.8 Hz, 3H); ES⁺ MS: 448 (M+1).

EXAMPLE Z-9 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide sodium salt

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (510 mg, 1.08 mmol) and (2S)-2-amino-1-propanol (0.17 mL, 2.17 mmol) were reacted in 1,2-dichloroethane (20 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-methyl-5,7-dioxo-6-[(phenylmethyl)oxy]-2, 3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (500 mg, 93%). This material was hydrogenated in a second step as described in example Z-1 to give 3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (386 mg, 94%) as a tinted white solid, ¹H NMR (CDCl₃) δ 11.46 (m, 1H), 10.28 (m, 1H), 8.32 (s, 1H), 7.35 (m, 1H), 6.80 (m, 2H), 5.30 (dd, J=10.0, 4.0 Hz, 1H), 4.63 (m, 2H), 4.48-4.37 (m, 3H), 3.91 (dd, J=12.0, 10.0 Hz, 1H), 8.73 (m, 1H), 1.48 (d, J=6.0 Hz, 3H); ES⁺ MS; 406 (M+1). This material (385 mg, 0.95 mmol) was treated with sodium hydroxide (0.95 mL, 1.0 M, 0.95 mmol) in ethanol (15 mL) as described in example Z-1 to provide its corresponding sodium salt (381 mg, 94%) as a white solid. ¹H NMR (DMSO-d₆) δ 10.66 (m, 1H), 7.93 (s, 1H), 7.33 (m, 1H), 7.20 (m, 1H), 7.01 (m, 1H), 5.19 (m, 1H), 4.59 (m, 1H), 4.48 (m, 2H), 4.22 (m, 2H), 3.75 (m, 1H), 3.57 (m, 1H), 1.24 (d, J=5.6 Hz, 3 H).

EXAMPLE Z-10 (3S,11aR)-N-[(4-Fluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16 (100 mg, 0.22 mmol) and (2S)-2-amino-1-propanol (0.10 mL, 1.28 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-N-[(4-fluorophenyl)methyl]-3-methyl-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (100 mg, 95%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-N-[(4-Fluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (80 mg, 99%) as a white solid. ¹H NMR (CDCl₃) δ 11.43 (br, 1H), 10.28 (br, 1H), 8.35 (s, 1H), 7.28 (m, 2H), 6.97 (m, 2H), 5.29 (m, 1H), 4.55-4.38 (m, 5H), 3.89 (apparent t, J=10.8 Hz, 1H), 3.70 (m, 1H), 1.45 (d, J=5.6 Hz, 3H); ES⁻ MS: 386 (M−1).

EXAMPLE Z-11 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-3-(1,1-dimethylethyl)-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (41 mg, 0.09 mmol) and freebased L-tert-leucinol (59 mg, 0.50 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-(1,1-dimethylethyl)-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a -hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (40 mg, 86%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-N-[(2,4-Difluorophenyl)metlayl]-3-(1,1-dimethylethyl)-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (33 mg, 99%) as a tinted white solid. ¹H NMR (CDCl₃) δ 10.29 (s, 1H), 8.37 (s, 1H), 7.34 (m, 1H), 6.79 (m, 2H), 5.43 (m, 1H), 4.62 (m, 2H), 4.36 (m, 2H), 4.21 (m, 1H), 3.99 (1H), 3.81 (m, 1H), 1.03 (s, 9H); ES⁺ MS: 448 (M+1).

EXAMPLE Z-12 (3S,11aR)-3-(1,1-Dimethylethyl)-N-[(4-fluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3 5,7,11,13a-hexahydroftaloxazolo[3,2-a]-pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2, 16 (41 mg, 0.09 mmol) and freebased L-tert-leucinol (59 mg, 0.50 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-3-(1,1-dimethylethyl)-N-[(4-fluorophenyl)methyl]-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (40 mg, 85%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,¹¹aR)-3-(1,1-Dimethylethyl)-N-[(4-fluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (32 mg, 97%) as a tinted white solid. ¹H NMR (CDCl₃) δ 11.15 (br, 1H), 10.32 (s, 1H), 8.38 (s, 1H), 7.29 (m, 2H), 6.98 (m, 2H), 5.43 (m, 1H), 4.58 (m, 2H), 4.36 (m, 2H), 4.21 (m, 1H), 3.99 (1H), 3.79 (m, 1H), 1.02 (s, 9 H); ES⁺ MS: 4.30 (M+1).

EXAMPLE Z -18 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-3-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (33 mg, 0.07 mmol) and L-phenylglycinol (19 mg, 0.14 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3S, 11aR)-N-[(4-fluorophenyl)methyl]-5,7 -dioxo-3-phenyl-6 -[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8carboxamide (37 mg, 95%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-3-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide (33 mg, 99%) as a tinted white solid. ¹H NMR. (CDCl₃) δ 11.23 (br, 1H), 10.27 (s, 1H), 8.39 (s, 1H), 7.43-7.32 (m, 6H), 6.80 (m, 2H), 5.58 (d, J=6.8 Hz, 1H), 5.37 (apparent t, J=6.8 Hz, 1H), 4.67-4.62 (m, 3H), 4.54 (d, J=10.4 Hz, 1H), 4.11 (m, 1H), 4.01 (m, 1H); ES⁺ MS: 468 (M+1).

EXAMPLE Z-14 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(hydroxymethyl)-5 7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (50 mg, 0.10 mmol) and (2R)-2-amino-3-[(phenylmethyl)oxy]-1-propanol (0.1 mL) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-6-[(phenylmethyl)oxy]-3-{[(phenyl methyl)oxy]methyl}-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (61 mg, 99%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3(hydroxymethyl)-5,7-dioxo-2,3, 5,7,11,11a-hexhydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide (37 mg, 87%) as a tinted white solid. ¹H NMR (CDCl₃/CD₃OD) δ 8.23 (s, 1H), 7.32 (m, 1H), 6.79 (m, 2H), 5.31 (d, J=7.6 Hz, 1H), 4.56 (s, 2H), 4.42-4.36 (m, 3 H), 4.17-4.11 (m, 2H), 3.85 (m, 1H), 3.62 (d, J=112 Hz, 1H).

EXAMPLE Z-15 (2S,3R)—N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2-phenyl-2,3,5,7,11,11ahexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (25 mg, 0.05 mmol) and (1S,2R)-(+)-norephedrine (0.1 mL) were reacted in dichloromethane (2 mL) with acetic acid to give (2S,3R)—N-[(2,4-difluorophenyl)methyl]-3-methyl-5,7-dioxo-2-phenyl-6-[(phenylmethyl) oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (30 mg, 99%). This material was hydrogenated in a second step as described in example Z-2 to give (2S,3R)—N-[(2,4-difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-c]pyrazine-8-carboxamide (25 mg, 91%) as a white solid, This material is a single diastereomer (>6:1 diastereomeric ratio but unconfirmed relative stereochemistry at the aminal center). ¹H NMR (CDCl₃/CD₃OD) δ 10.28 (m, 1H), 8.38 (s, 1H), 7.10-7.30 (m, 6H), 6.78 (m, 2H), 5.70 (d, J=7.6 Hz, 1H), 5.36 (d, J=5.2 Hz, 1H), 4.82 (m, 1H), 4.61 (m, 2H), 4.47 (d, J=10.4 Hz, 1H), 4.00 (apparent t, J=10.4 Hz, 1H), 0.94 (d, J=6.4 Hz, 3H); ES⁺ MS: 482 (M+1).

EXAMPLE Z-16 (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-3-(phenylmethyl)-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2, 16a (34 mg, 0.07 mmol) and (2R)-2-amino-3-phenyl-1-propanol (D-phenylalaninol) (50 mg, 0.33 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3R, 11aS)-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-3-(phenylmethyl)-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (29 mg, 70%). This material was hydrogenated in a second step as described in example Z-2 to give (3R, 11a S)-N-[(2,4-Difluorophenyl)methyl]-6 -hydroxy-5,7-dioxo-3-(phenylmethyl)-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (24 mg, 98%) as a white solid. ¹H NMR (CDCl₃) δ 11.46 (br, 1H), 10.27 (m, 1H), 8.33 (m, 1H), 7.32-7.16 (m, 6H), 6.78 (m, 2H), 5.02 (m, 1H), 4.61 (m, 3H), 4.39 (m, 1H), 4.22 (m, 1H), 3.95 (m, 1H), 3.87 (m, 1H), 3.36 (m, 1H), 2.97 (dd, J=13.2 8.8 Hz, 1H); ES⁺ MS: 482 (M+1).

EXAMPLE Z-17 (3R,11aS)-N-[(2,4-Difluorophenyl)methy]-6-hydroxy-3-(2-methylpropyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[3,2-a]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z2. 16a (32 mg, 0.07 mmol) and (2R)-2-amino-4-methyl-1-pentanol (0.1 mL) were reacted in dichloromethano (2 mL) with acetic acid to give (3R,11aS)-N-[(2,4-difluorophenyl)methyl]-3-(2-methylpropyl)-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (43 mg, 99%). This material was hydrogenated in a second step as described in example Z-2 to give (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(2-methylpropyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (32 mg, 90%) as a white solid. ¹H NMR (CDCl₃) δ 11.47 (br, 1H), 10.29 (m, 1H), 8.35 (s, 1H), 7.39 (m, 1H), 6.80 (m, 2H), 5.31 (m, 1H), 4.62 (m, 2H), 4,44 (m, 2H), 4.37 (m, 1H), 3.88 (m, 1H), 3.84 (dd, J=8.0, 5.6 Hz, 1H), 2.04 (m, 1H), 1.62 (m, 1H), 1.41 (m, 1H), 1.00 (d, 5.6 Hz, 3H), 0.99 (d, J=6.0 Hz, 3H); ES⁺ MS: 448 (M+1).

EXAMPLE Z-18 (5aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide

-   a) 1,1-Dimethylethyl (2R)-2-(aminocarbonyl)-1-piperidinecarboxylate.     To a cold (0° C.) solution of     (2R)-1-{[(1,1-dimethylethyl)oxy]carbonyl}-2-piperidinecarboxylic     acid (1.0 g, 4.36 mmol) in THF (20 mL) was added triethylamine (0.60     mL, 4.36 mmol) followed by slow addition of methyl chloroformate     (0.34 mL, 4.36 mmol). After a few minutes a suspension had formed.     To this mixture was added concentrated NH₄OH (1.5 mL) and the     solution was allowed to warm to rt as the bath warmed and stirred     for a total of 4 h. The mixture was concentrated in vacuo and the     residue was taken up in EtOAc. The organic layer was washed with     citric acid, bicard and then brine, dried over Na₂SO₄. Filtration     and concentration gave 1,1-dimethylethyl     (2R)-2-(aminocarbonyl)-1-piperidinecarboxylate (1.0 g, 99%). ¹H NMR     (CDCl₃) δ 6.03 (br, 1H), 5.45 (br, 1H), 4.77 (br, 1H), 4.06 (br,     1H), 2.82 (m, 1H), 2.29 (m, 1H), 1.67-1.43 (m, 13H), -   b) 1,1-Dimethylethyl (2R)-2-cyano-1-piperidinecarboxylate. To a cold     (0° C.) solution of 1,1-dimethylethyl     (2R)-2-(aminocarbonyl)-1-piperidinecarboxylate (269 mg, 1.17 mmol in     THF (10 mL) was added triethylamine (0.33 mL, 2.34 mmol) and then     trifluoroacetic anhydride (0.17 mL, 1.17 mmol). The mixture was     stirred at 0° C. for 1 h and concentrated in vacuo. The residue was     taken up in EtOAc and washed successively with sodium bicarbonate,     0.5 N HCl and brine. The organics were dried over Na₂SO₄, filtered     and concentrated to give 1,1-dimethylethyl     (2R)-2-cyano-1-piperidinecarboxylate (255 mg, 99%) as a crystalline     solid upon standing, ¹H NMR (CDCl₃) δ 5.23 (br, 1H), 4.05 (br, 1H),     2.93 (br, 1H), 1.93-1.39 (m, 6H), 1.46 (s, 9H). -   c) 1,1-Dimethylethyl (2R)-2-(aminomethyl)-1-piperidinecarboxylate.     An ammonia saturated ethanol solution of 1,1-dimethylethyl     (2R)-2-cyano-1-piperidinecarboxylate (255 mg, 1.19 mmol) was reduced     with Raney-Ni in a similar manner to that described in example Z-3     to give after filtration through a short plug of silica,     1,1-dimethylethyl (2R)-2-(aminomethyl)-1-piperidinecarboxylate (236     mg, 91%), as an oil, ¹H NMR (CDCl₃/CD₃OD) δ 4.15 (br, 1H), 3.97 (m,     1 h), 2.96 (m, 1H), 2.75-2.69 (m, 2H), 2.23-2.08 (m, 3H), 1.59-1.55     (m, 3H), 1.43 (s, 9H). -   d) [(2R)-2-Piperidinylmethyl]amine bis HCl salt. A solution of     1,1-dimethylethyl (2R)-2-(aminomethyl)-1-piperidinecarboxylate (236     mg, 1.08 mmol) in THF (10 mL) was treated with 4 N HCl (3 mL) as     described in example Z-3 to give the his HCl salt of     [(2R)-2-Piperidinylmethyl]amine. ¹H NMR (DMSO-d₆) δ 9.67 (br, 1H),     9.48 (br, 1H), 8.48 (br, 2H), 3.70 (br, 2H), 3.20 (m, 1H), 3.04 (m,     1H), 2.86 (m, 1H), 1.89-1.41 (m, 6H). -   e)

(5aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (50 mg, 0.11 mmol) and [(2R)-2-Piperidinylmethyl]amine (150 mg, 1.31 mmol) (free based using carbonate resin as described in example Z-3) were reacted in dichloromethane (2 mL) with acetic acid to give (5aR,14aR)-N-[(2,4-difluorophenl)methyl]-10,12-dioxo-11-[(phenylmethyl)oxy]-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide (50 mg, 88%). This material was hydrogenated in a second step as described in example 2.2 to give (5aR,14aR)-N-[(2,4-difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-a]pyrazine-9-carboxamide (11 mg, 44%) as a white solid, ¹H NMR (CD₃OD/CDCl₃) δ 10.46 (m, 1H), 8.32 (s, 1H), 7.31 (m, 1H), 6.80 (m, 2H), 4.64-4.52 (m, 3H), 4.14 (dd, J=10.4, 2.8 Hz, 1H), 3.91-3.82 (m, 2H), 3.19 (apparenrt, J=10.8 Hz, 1H), 3.08 (d, J=10.4 Hz, 1H), 2.50 (m, 1H), 2.27 (m, 1H), 1.99-1.30 m, 6 H); ES⁺ MS: 445 (M+1).

EXAMPLE Z-19 (2S,3S)—N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(methyloxy)methyl]-5,7-dioxo-2-phenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (36 mg, 0.07 mmol) and (2R)-2-amino-4-methyl-1-pentanol (0.1 mL) were reacted in dichloromethane mL) with acetic acid to give (2S,3S)—N-[(2,4-difluorophenyl)methyl]-3-[(methyloxy)methyl]-5,7-dioxo-2-phenyl-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2a]pyrido[1,2-d]pyrazine-8-carboxamide. This material was hydrogenated in a second step as described in example Z-2 to give (2S,3S)—N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(methyloxy)methyl]-5,7-dioxo-2-phenyl-2,3,5,7,11,11a -hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (26 mg, 64% for 2 steps) as a white solid. This material is a single diastereomer (>6:1 diastereomeric ratio but unconfirmed relative stereochemistry at the aminal center). ¹H NMR (CDCl₃) δ 11.48 (hr, 1H), 10.30 (m, 1H), 8.39 (s, 1H), 7.39-7.24 (m, 6H), 6.78 (m, 2H), 5.46 (dd, J=10.0, 3.6 Hz, 1H), 5.33 (d, J=7.2 Hz, 1H), 4.63 (m, 2H), 4.54 (dd, J=12,4, 4.0 Hz, 1H), 4.19 (m, 1H), 4.12 (dd, J=10.4, 3.2 Hz, 1H), 4.06 (m, 1H), 3.55 (dd, J=10.4, 1.6 Hz, 1H), 3.40 (s, 3H); ES⁺MS: 512 (M+1).

EXAMPLE Z-20 (3S,11aR)-3-(Cyclohexylmethyl)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (36 mg, 0.08 mmol) and (2S)-2-amino-3-cyclohexyl-1-propanol (30 mg, 0.19 mmol) were reacted in dichloromethane mL) with acetic acid to give (3S,11aR)-3-(cyclohexylmethyl)-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (27 mg, 61%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-3-(cyclohexylmethyl)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (25 mg, 99%) as a white solid. ¹H NMR (CDCl₃) δ 11.48 (br, 1H), 10.28 (s, 1H), 8.33 (s, 1H), 7.33 (m, 1H), 6.78 (m, 2H), 5.29 (m, 1H), 4.61 (m, 2H), 4.47-4.33 (m, 3H), 3.87-3.81 (m, 2H), 2.05 (m, 1H), 1.75-1.64 (m, 6H), 1.39 (m, 1H), 1.25-1.14 (m, 3H), 1.02-0.97 (m, 2H); ES⁺ MS: 488 (M+1).

EXAMPLE Z-21 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(1-methylethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (42 mg, 0.09 mmol) and (2S)-2-amino-3-methyl-1-butanol (0.1 mL) were reacted in 1,2-dichloroethane (8 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-(1-methylethyl)-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyraine-8-carboxamide (40 mg, 86%). This material was hydrogenated in a second step as described in example Z-1 to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-3-(1-methylethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-c]pyrido[1,2-d]pyrazine-8-carboxamide (34 mg, 99%) as a white solid. ¹H NMR (CDCl₃) δ 10.29 (br, 1H), 8.36 (s, 1H), 7.33 (m, 1H), 6.79 (m, 2H), 5.29 (d, J=6.4 Hz, 1H), 4.61 (m, 2H), 4.44 (d, J=9.6 Hz, 1H), 4.34 (m, 1H), 4.17 (m, 1H), 4.02 (dd, J=8.4, 5.2 Hz, 1H), 3.86 (m, 1H), 2.37 (m, 1H), 0.97 (m, 6 H); ES⁺ MS: 434 (M+1).

EXAMPLE Z-22 (5aR14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-5a,6a 7,11,13,14a-hexahydro-5H-indeno[1′,2′:4,5][1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-10-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (42 mg, 0.09 mmol) and (1S2R)-1-amino-2,3-dihydro-1H-inden-2-ol (100 mg, 0.67 mmol) were reacted in 1,2-dichloroethane mL) with acetic acid to give (5aR,14aS)-N-[(2,4-difluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-5a,6a,711,13,14a-hexahydro-5H-indenao[1′,2′:4,5:][1,3]oxazolo[3,2-a]pyrido[1,2-d]pyra zine-10-carboxamide (55 mg, 99%). This material was hydrogenated in a second step as described in example Z-1 to give (5aR,14aS)-N-[(2,4-difluorophenyl) methyl]-12-hydroxy-11,13-dioxo-5a,6a,7,11,13,14a-hexahydro-5H-indeno[1′,2′:4,5][1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-10-carboxamide (45 mg, 97%) as a white solid. ¹H NMR (CDCl₃) δ 10.28 (m, 1H), 8.33 (s, 1H), 7.69 (d, J=7.2 Hz, 1H), 7.34-7.19 (m, 4H), 6.78 (m, 2H), 5.96 (d, J=6.0 Hz, 1H), 5.32 (m, 1H), 5.22 (m, 1H), 4.60 (m, 2H), 4.45 (d, J=9.2 Hz, 1H), 3.96 (apparent t, J=10.8 Hz, 1H), 3.40 (dd, J=18.0, 6.8 Hz, 1H), 3.24 (d, J=17.6 Hz, 1H);); ES⁺ MS: 480 (M+1).

EXAMPLE Z-23 & Z-24 (2S,3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3-dinhenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide & (2S,3R,11aR)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3-diphenyl-2,3,5,7,11,11a-hexahydra[1,3]oxazolo[3,2-a]ptrido[1,2-d]pyrazine-8-carboxamide

The title compounds were made in two steps using a similar process to that described in example Z-1, 16a (40 mg, 0.09 mmol) and (1S,2R)-2-amino-1,2-diphenylethanol (50 mg, 0.23 mmol) were reacted in 1,2-dichloroethane (5 mL) with acetic acid to give (2S,3R,11aS)-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-2,3-diphenyl-6-[(phenylmethyl) oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (34 mg, 63%) and (2S,3R,11aR)-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-2,3-diphenyl-6-[(phenylmethyl) oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (13 mg, 24%). These materials were hydrogenated in a second step as described in example Z-1 to give (2S,3R,11aS)-N-[(2, 4-Difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3-diphenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (example Z-23, 29 mg, 99%) as a white solid and (2S,3R,11aR)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3-diphenyl-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (example Z-24, 10 mg, 89%) as a white solid respectively. For example Z-23: ¹H NMR (DMSO-d₆) δ 10.29 (t, J=5.6 Hz, 1H), 8.55 (s, 1H), 7.38 (m, 1. H), 7.22 (m, 1H), 7.11-6.95 (m, 11H), 6.16 (dd, J=10.4, 3.6 Hz, 1H), 5.71 (m, 2H), 4.90 (m, 1H), 4.54 (m, 2H), 4.38 (t, J=11.2 Hz, 1H); ES⁺ MS: 544 (M+1). For example Z-24: ¹H NMR (CDCl₃) δ 11.64 (br, 1H), 10.30 (s, 1H), 8,45 (s, 1H), 7.34 (m, 1H), 7.01-6.90 (m, 10H), 6.80 (m, 2H), 5.56 (m, 2H), 5.42 (d, J=6.4 Hz, 1H), 4.73 (m, 1H), 4.63 (m, 2H), 4.49 (m, 1H); ES⁺ MS: 544 (M+1).

EXAMPLE Z-25 (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(1-methylethyl)-5,7-dioxo-2,3,5,7,11,11a-hexalaydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (40 mg, 0.09 mmol) and (2R)-2-amino-3-methyl]-1-butanol (0.1 mL) were reacted in 1,2-dichloroothane (8 mL) with acetic acid to give (3R,11aS)-N[(2,4-difluorophenyl)methyl]-3-(1-methylethyl)-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (41 mg, 92%). This material was hydrogenated in a second step as described in example Z1 to give (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(1-methylethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (32 mg, 94%) as a white solid. ¹H NMR (CDCl₃) δ 11.42 (br, 1H), 10.27 (br, 1H), 8.34 (s, 1H), 7.31 (m, 1H), 6.78 (m, 2H), 5.28 (d, J=6.0 Hz, 1H), 4.60 (m, 2H), 4.42 (m, 1H), 4.33 (m, 1H), 4.16 (m, 1H), 4.01 (dd, J=8.8, 5.2 Hz, 1H), 3.85 (m, 1H), 2.37 (m, 1H), 0.97 (d, J=6.8 Hz, 3H), 0.95 (d, J=6.4 Hz, 3H); ES⁺ MS: 434 (M+1).

EXAMPLE Z-26 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[2-(methylthio)ethyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (43 mg, 0.09 mmol) and (2S)-2-amino-4-(methylthio)-1-butanol (0.1 mL) were reacted in 1,2-dichloroethane (5 mL) with acetic acid to give (3S,11aR-N-[(2,4,-difluorophenyl)methyl]-3-[2-(methylthio)ethyl]-5,7-dioxo-6-[(phenyl methyl)oxy]-2,3,5,7,11,11a -hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (41 mg, 81%). This material (20 mg, 0.04 mmol) was treated with trifluoroacetic acid (1 mL) in dichloromethane (3 mL) at 0° C. to rt over 6 h. The mixture was concentrated in vacuo and subjected to reverse phase preparative HPLC purification to provide (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[2-(methylthio)ethyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide (12 mg, 72%) as a white solid. ¹H NMR (CDCl₃) δ 11.35 (br, 1H), 10.25 (s, 1H), 8.34 (s, 1H), 7.33 (m, 1H), 6.79 (m, 2H), 5.32 (m, 1H), 4.62-4.53 (m, 3 H), 4.43-4.39 (m, 2H), 3.91-3.87 (m, 2H), 2.63-2.53 (m, 2H), 2.39 (m, 1H), 2.12 (s, 3 H), 1.89 (m, 1H); ES⁺ MS: 466 (M+1).

EXAMPLE Z-27 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[2-(methylsulfonyl)ethyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

To a solution of (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-[2-(methylthio)ethyl]-5,7-dioxo-6-[(phenyl methyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (20 mg, 0.04 mmol) in dichloromethane (5 mL) at 0° C. was added m-CPBA (20 mg, 70%, 0.082 mmol). The resultant solution was allowed to warm as the bath warmed and stirred a total of 3 h. The reaction was quenched by the addition of Na₂S₂O₃ (aq) and sodium bicarbonate. The layers were separated and the organic layer washed with brine. The aqueous layer was extracted with dichloromethane and the combined organics dried over Na₂SO₄. Filtration and concentration provided (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-[2-(methylsulfonyl)ethyl]-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (26 mg, 99%) as a white solid. This material was hydrogenated in a second step as described in example Z-1 to give (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[2-(methylsulfonyl)ethyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-a]pyrazine-8-carboxamide (22 mg, 99%) as a white solid. ¹H NMR (CDCl₃) δ 11.00 (br, 1H), 10.16 (s, 1H), 8.83 (s, 1H), 7.36 (m, 1H), 6.81 (m, 2H), 5.42 (m, 1H), 4.62 (m, 3H), 4.41 (m,2H), 3.93 (m, 2H), 3.31 (m, 2H), 2.98 (s, 3H), 2.40 (m, 1H), 2.28 (m, 1H); ES⁺ MS: 498 (M+1).

EXAMPLE Z-28 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-(1H-indol-3-ylmethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[8,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-1. 16a (4.3 mg, 0.09 mmol) and (2S)-2-amino-3-(1H-indol-3-yl)-1-propanol (100 mg, 0.52 mmol) were reacted in 1,2-dichloroethane 5 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-(1H-indol-8-ylmethyl)-5,7-dioxo-6-[(phenyl methyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (36 mg, 64%). This material was hydrogenated in a second step as described in example Z-1 to give (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy3-(1H-indol-3-ylmethyl)-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (29 mg, 95%) as a white solid. ¹H NMR (CDCl₃/CD₃OD) δ 10.34 (m, 1H), 8.98 (br, 1H), 8.24 (s, 1H), 7.58 (d, J=8.0 Hz, 1H), 7.32 (m, 2H), 7.15-7.01 (m, 3H), 6.78 (m, 2H), 4.94 (d, J=6.8 Hz, 1H), 4.71 (d, J=5.6 Hz, 1H), 4.59 (m, 2H), 4.35 (d, J=10.4 Hz, 1H), 4.22 (m, 1H), 3.99 (m, 1H), 3.81 (m, 1H), 3.40 (dd, J=13.6, 11.6 Hz, 1H), 3.18 (dd, J=14.0, 8.4 Hz, 1H); ES⁺ MS: 521 (M+1).

EXAMPLE Z-29 (4R,12aR)-N-[(4-fluorophenvl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydroxyrido[1′,2′;4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) (2R)-2-({[1,1-Dimethylethyl)oxy]carbonyl}amino)propyl     methanesulfonate. To a stirred solution of 1,1-dimethylethyl     [(1R)-2-hydroxy-1-methylethyl]carbamate (5.00 g, 28.5 mmol) and     triethylaMine (5.92 mL, 42.9 mmol) in CH₂Cl₂ (30 mL) cooled to 0° C.     and under a nitrogen atmosphere was added dropwise a solution of     methanesulfonyl chloride (2.43 mL, 31.5 mmol) in CH₂Cl₂ (25 mL).     Stirring was continued for 20 minutes at 0° C., after which time the     reaction was judged complete by TLC analysis (1:1 bexanes/EtOAc).     The solution was poured into water and the layers were separated.     The organic phase was washed with 0.1 N HCl and then with 5% NaHCO₃,     dried over Na₂SO₄, filtered and concentrated to give     (2R)-2-({[(1,1-dimethylethyl)oxy]lcarbonyl}amino)propyl     methanesulfonate (7.08 g, 98%) as a white solid. ¹H NMR (400 MHz,     CDCl₃) δ 1.23 (d, J=6.8 Hz, 3H), 1.44 (s, 9H), 3.03 (s, 3H), 3.97     (m, 1H), 4.15 (dd, J=4.2, 9.8 Hz, 1H), 4.21 (m, 1H), 4.61 (br s,     1H). -   b) 1,1-Dimethylethyl [(1R)-2-cyano-1-methylethyl]carbamate. To a     stirred solution of     (2R)-2-({[(1,1-dimethylethyl)oxy]carbonyl}amino)propyl     methanesulfonate (7.08 g, 27.9 mmol) in DMSO (50 mL) was added NaCN     (3.78 g, 84.0 mmol). The solution was stirred at 70° C. for 2 hours,     over which time the formation of a precipitate was observed. After     cooling at room temperature, water was added and the mixture was     extracted with Et₂O. The ethereal layers were washed with a brine     solution, dried over Na₂SO₄, filtered and concentrated to give     1,1-dimethylethyl [(1R)-2cyano-1-methylethyl]carbamate (3.81 g, 73%)     as a pale yellow solid. ¹H NMR (400 MHz, CDCl₃) δ 1.30 (d, J=6.8 Hz,     3H), 1.42 (s, 9H), 2.51 (dd, J=3.8, 16.6 Hz, 1H), 2.73 (m, 1H), 3.93     (m, 1H), 4.63 (br s, 1H). -   c) 1,1-Dimethylethyl [(1R)-3-amino-1-methylpropyl]carbamate. A     solution of 1,1-dimethylethyl [(1R-2-cyano-1-methylethyl]carbamate     (1.30 g, 7.1 mmol) in ethanol saturated with anhydrous ammonia was     treated with Raney-Ni (1.5 mL of 50% aq. Suspension) and 55 psi of     H₂ overnight. The mixture was filtered through Celite and the     filtrate was concentrated in vacuo. The residue was purified by     flash chromatography (80:19:1 CH₂Cl₂/MeOH/NH₄OH (37%) gradient     elution) through a short plug of silica gel to give     1,1-dimethylethyl [(1R)-3-amino-1-methylpropyl]carbamate (1.37 g,     100%) as a clear oil that solidified. ¹H NMR (400 MHz, CDCl₃) δ 11.4     (d, J=6.8 Hz, 3H), 14.3-1.62 (m, 13H), 2.76 (m, 2H), 3.77 (m, 1H),     4.57 (m, 1H). -   d) 1,1-Dimethylethyl     {(1R)-1-methyl-3-{(2-methylpropyl}amino]propyl}carbamate,     1,1-dimethylethyl [(1R)-3-amino-1-methylpropyl]carbamate (0.320 g,     1.70 mmol), isobutyraldehyde (150 μL, 1.62 mmol), and sodium     triacetoxyborohydricle (0.512 g, 2.42 mmol) were stirred in     anhydrous dichloroethane (10 mL) at ambient temperature overnight.     The reaction was quenched by the addition of saturated NaHCO₃ and     then extracted with dichloroinethane. The combined extracts were     washed with water, dried over Na₂SO₄, filtered and concentrated. The     residue was purified by flash chromatography (80:19:1     CH₂Cl₂/MeOH/NH₄OH (37%) gradient elution) through a short plug of     silica gel to afford 1,1-dimethylethyl     {(1R)-1-methyl-3-[(2-methylpropyl)amino]propyl}carbamate (0.158 g,     40%) as a clear oil. ¹H NMR (400 MHz, CDCl₃) δ 0.90 (d, J=6.4 Hz,     6H), 1.13 (d, J=6.4 Hz, 3H), 1.42-1.51 (m, 1H), 1.67-1.75 (m, 2H),     2.33-2.42 (m, 2H), 2.58-2.72 (m, 2H), 3.72 (m, 1H), 5.20 (m, 1H). -   e) [(3R)-3-Aminobutyl](2-methylpropyl)amine. An ice cold solution of     1,1-dimethylethyl     {(1R)-1-methyl-3-[(2-methylpropyl)amine]propyl}carbamate (0.158 g,     0.65 mmol) in THF (8 mL) was treated with 4 N HCl (aq) (2 mL) and     then stirred at room temperature for 2 h. The mixture was     concentrated in vacuo to give     [(3R)-3-aminobutyl](2-methylpropyl)amine dihydrochloride. The HCl     salt was then dissolved in dichloromethane and a minimal amount of     methanol and treated with solid supported carbonate resin     (MP-Carbonate, Argonaut Technologies). After 30 minutes, the     solution was filtered through a fritted tube and the solvents     removed carefully in vacuo to give     [(3R)-3-aminobutyl](2-methylpropypamine (65 mg). ¹H NMR (400 MHz,     CDCl₃) δ 0.88 (d, J=6.0 Hz, 6H), 1.06 (d, J=5.6 Hz, 3H), 1.23-1.53     (m, 5H), 1.71-1.74 (m, 1H), 2.39 (m, 2H), 2.65 (m, 2H), 2.97 (m,     1H). -   f)

(4R,12aR)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylprepyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2, 16 (40 mg, 0.09 mmol) and [(3R)-3-aminobutyl](2-methylpropyl)amine (65 mg, 0.45 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4R,12aR)-N-[(4-fluorophenyl)methyl]-4-methyl-1-(2methylpropyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (29 mg, 60%), This material was hydrogenated in a second step as described in example Z-2 to give (4R,12aR)-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (18 mg, 75%) as a tan solid. ¹H NMR (400 MHz, CDCl₃) δ 0.77 (d, J=6.4 Hz, 3H), 0.84 (d, J=6.4 Hz, 3H), 1.32 (d, J=7.2 Hz), 1.45-1.49 (m, 1H), 1.57-1.67 (m, 1H), 2.03-2.12 (m, 2H), 2.21-2.27 (m, 1H), 2.73-2.79 (m, 1H), 2.87-2.92 (m, 1H), 4.16-4.24 (m, 2H), 4.45 (s, 1H), 4.54-4.64 (m, 2H), 4.96-4.99 (m, 1H), 6.96-7.00 (m, 2H), 7.29-7.32 (m, 2H), 8.27 (s, 1H), 10.46 (s, 1H), 12.55 (s, 1H); ES⁺ MS: 456 (M+1). EXAMPLE Z-30 (4R,12aR)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3R)-3-Aminobutyl](1-methylethyl)amine. The free diamine was     prepared in a similar manner as described in example Z-29. ¹H NMR     (400 MHz, CDCl₃) δ 1.04 (d, J=6.4 Hz, 6H), 1.06 (d, J=6.4. Hz, 3H),     1.41-1.58 (m, 5H), 2.62-2.66 (m, 2H), 2.74-2.80 (m, 1H), 2.92-3.00     (m, 1H). -   b)

(4R,12aR)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16 (40 mg, 0.088 mmol) and [(3R)-3-aminobutyl](1-methylethyl)amine (78 mg, 0.60 mmol) were reacted in dichloromethane (2 with acetic acid to give (4R,12aR)-N-[(4-fluorophenyl)methyl]-4-methyl-1-(1-methylethyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (26 mg, 56%). This material was hydrogenated in a second step as described in example Z-2 to give (4R,12aR)-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methyletbyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (21 mg, 90%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.01 (d, J=5.6 Hz, 3H), 1.06 (d, J=6.0 Hz, 3H), 1.31 (d, J=6.8 Hz, 3H), 1.57 (m, 1H), 1.98 (m, 1H), 2.70-2.82 (m, 2H), 3.16 (m, 1H), 4.15-4.19 (m, 1H), 4.30 (m, 1H), 4.48 (s, 1H), 4.54-4.59 (m, 2H), 4.97 (m, 1H), 6.98 (m, 2H), 7.29-7.32 (m, 2H), 8.27 (s, 1H), 10.49 (s, 1H), 12.52 (s, 1H). EXAMPLE Z-31 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxo-1,2,14,1,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) 1,1-Dimethylethyl [(1S)-2-cyano-1-methylethyl]carbamate. The     nitrile was prepared in two steps using a modified procedure as     described in example Z-29. To a stirred solution of     (2S)-2-({[(1,1-dimethylethyl)oxy]carbonyl}amino)propyl     methanesulfonate (8.40 g, 33.2 mmol) in DMSO (50 mL) and KCN (6.51     g, 100.0 mmol) cooled to 0° C. was added 18-crown-6 (9.05 g, 34.3     mmol). The solution was allowed to warm to room temperature and then     heated to 70° C. for 1 hour. After cooling at room temperature,     water was added and the mixture was extracted with Et₂O. The     ethereal layers were washed with a brine solution, dried over     Na₂SO₄, filtered and concentrated to give 1,1-dimethylethyl     [(1S)-2-cyano-1-methylethyl]carbamate (5.37 g, 88%) as a pale yellow     solid. ¹H NMR (400 MHz, CDCl₃) δ 1.32 (d, J=6.8 Hz, 3H), 1.44 (s,     9H), 2.52 (dd, J=4.0, 16.4 Hz, 1H), 2.74 (m, 1H), 3.95 (m, 1H), 4.65     (br s, 1H). -   b) [(3S)-3-Aminobutyl](2-methylpropyl)amine dihydrochloride was     prepared in a similar manner as described in example Z-29. ¹H NMR     (400 MHz, CDCl₃/CD₃OD) δ 0.99 (m, 6H), 1.34 (m, 3H), 2.13-2.27 (m,     3H), 2.76 (m, 2H), 3.07 (m, 2H), 3.47 (m, 1H), 8.22 (m, 1H), 8.83     (m, <1H). -   c)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (80 mg, 0.17 mmol) and free based [(3S)-3-aminobutyl](2-methylpropyl)amine (107 mg, 0.74 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-4-methyl-1-(2-methylpropyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (76 nig, 76%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxol-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (39 mg, 80%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 0.76 (d, J=6.4 Hz, 3H), 0.84 (d, J=6.4 Hz, 3H), 1.32 (d, J=7.2 Hz, 3H), 1.45-1.50 (m, 1H), 1.60-1.69 (m, 1H), 2.03-2.12 (m, 2H), 2.21-2.27 (m, 1H), 2.73-2.79 (m, 1H), 2.87-2.93 (m, 1H), 4.16-4.25 (m, 2H), 4.45 (s, 1H), 4.57-4.68 (m, 2H), 4.96-5.01 (m, 1H), 6.75-6.82 (m, 2H), 7.32-7.38 (m, 1H), 8.26 (s, 1H), 10.45 (s, 1H), 12.56 (s, 1H); ES MS: 475 (M+1).

EXAMPLE Z-32 (4S,12aS)-1-(Clyclopropylmethyl)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) 1,1-Dimethylethyl     {(1S)-3-[(cyclopropylmethyl)amino]-1-methylpropyl}carbamate, The     protected diamine was prepared using a modified procedure as     described in example Z-29. 1, 1-dimethylethyl     [(1S)-3-amino-1-methylpropyl]carbamate (0.293 g, 1.56 mmol),     cyclopropane carboxaldehyde (96 μL, 1.30 mmol), and sodium     triacetoxyborohydride (0.439 g, 2.07 mmol) were stirred in a 1:1     mixture of anhydrous dichloroethane and tetrahydrofuran (10 mL) at     ambient temperature overnight. The reaction was quenched by the     addition of saturated NaHCO₃ and then extracted with EtOAc. The     combined extracts were washed with saturated NaHCO₃, then a solution     of brine, dried over Na₂SO₄, filtered and concentrated. The residue     was purified by flash chromatography (80:19:1 CH₂Cl₂/MeOH/NH₄OH     (37%) gradient elution) through a short plug of silica gel to afford     1,1-dimethylethyl     {(1S)-3-[(cyclopxopylmethyl)amino]-1-methylpropyl}carbamate (76 mg,     26%) as a clear oil, ¹H NMR (400 MHz, CDCl₃) δ 0.09-0.13 (m, 2H),     0.44-0.49 (m, 2H), 0.92-0.95 (m, 1H), 1.14 (d, J=6.4 Hz, 3H),     1.43-1.70 (m, 12H), 2.38-2.50 (m, 2H), 2.62-2.73 (m, 2H), 3.74 (m,     1H), 4.88 (m, 1H). -   b) [(3S)-3-Aminobutyl](cyclopropylmethyl)amine dihydrochloride was     prepared in a similar manner as described in example Z-29. ¹H NMR     (4.00 MHz, CDCl₃/CD₃OD) δ 0.40 (m, 2H), 0.64 (m, 2H), 1.15 (m, 1H),     1.34 (m, 3H), 2.12-2.25 (m, 2H), 2.82 (m, 2H), 3.08 (m, 2H), 3.47     (m, 1H), 8.25 (br, <1H), 9.04 (br, <1H). -   c)

(4S,12aS)-1-(Cyclopropylmethyl)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9 -carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (50 mg, 0.106 mmol) and free based [(3S)-3-aminobutyl](cyclopropylmethyl)amine (44 mg, 0.31 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-1-(cyclopropylmethyl)-N-[(2,4-difluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (50 mg, 83%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-1-(cyclopropylmethyl)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (23 mg, 56%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 0.1.1 (m, 2H), 0.56-0.59 (m, 2H), 0.77 (m, 1H), 1.34 (d, J=7.2 Hz, 3H), 1.46-1.50 (m, 1H), 2.04-2.13 (m, 1H), 2.30-2.34 (m, 1H), 2.46-2.51 (m, 1H), 2.90-2.96 (m, 1H), 3.16-3.19 (m, 1H), 4.21-4.30 (m, 2H), 4.51 (s, 1H), 4.58-4.67 (m, 2H), 5.00-5.05 (m, 1H), 6.75-6.82 (m, 2H), 7.31-7.37 (m, 1H), 8.28 (s, 1H), 10.46 (s, 1H), 12.55 (br, 1H); ES⁺ MS: 473 (M+1).

EXAMPLE Z-33 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-1-(2-furanylmethyl)-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl](2-furanylmethyl)amine dihydrochloride was     prepared in a similar manner as described in example Z-32, ¹H NMR     (400 MHz, CDCl₃/CD₃OD) δ 1.27 (d, J=6.4 Hz, 3H), 1.96-2.05 (m, 1H),     2.14-2.19 (m, 1H), 3.00-3.04 (m, 2H), 3.38-3.39 (m, 1H), 4.11-4.18     (m, 2H), 6.34 (m, 1H), 6.59 (m, 1H), 7.40 (m, 1H), 8.18 (br, <1 14),     9.41 (br, <1H). -   b)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-1-(2-furanylmethyl)-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (36 mg, 0.076 mmol) and free based [(3S)-3-aminobutyl](2-furanylmethyl)amine (70 mg, 0.42 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-1-(2-furanylmethyl)-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (32 mg, 70%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-1-(2-furanylmethyl)-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (20 mg, 76%), as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.24 (d, J=6.8 Hz, 3H), 1.45-1.49 (m, 1H), 2.04-2.13 (m, 1H), 2.77-2.82 (m, 1H), 2.94-3.01 (m, 1H), 3.65 (d, J=15.6 Hz, 1H), 3.89 (d, J=16.0 Hz, 1H), 4.27-4.31 (m, 1H), 4.39-4.41 (m, 1H), 4.49-4.53 (m, 1H), 4.58-4.66 (m, 1H), 4.98-5.03 (m, 1H), 6.24 (m, 1H), 6.36 (m, 1H), 6.75-6.82 (m, 2H), 7.31-7.39 (m, 1H), 7.40 (m, 1H), 8.26 (s, 1H), 10.47 (m, 1H), 12.50 (br, 1H); ES⁺ MS: 499 (M+1).

EXAMPLE Z-34 (1S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(1,3-thiazol-2-ylmethyl)-1,2,1,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine9-carboxamide

-   a) [(3S)-3-Aminobutyl](1,3-thiazol-2-ylmethyl)amine dihydrochloride     was prepared in a similar manner as described in example Z-32. ¹H     NMR (400 MHz, CDCl₃/CD₃-OD) δ 1.28 (d, J=6.4 Hz, 3H), 2.05 (m, 1H),     2.17 (m, 1H), 3.20 (m, 2H), 3.39 (m, 1H), 4.51-4.58 (m, 2H), 7.52     (d, 1H), 7.82 (d, 1H). -   b)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(1,3-thiazol-2-ylmethyl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (35 mg, 0.074 mmol) and free based [(3S)-3-aminobutyl](1,3-thiazol-2-ylmethyl)amine were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1-(1,3-thiazol-2-ylmethyl)-1,2,8,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (36 mg, 80%) as a film, This material was debenzylated in a second step to in a manner similar to Z-26 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(1,3-thiazol-2-ylmethyl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (18 mg, 60%) as an off-white solid. ¹H NMR, (400 MHz, CDCl₃) δ 1.30 (d, J=7.2 Hz, 3H), 1.49-1.53 (m, 1H), 2.12-2.18 (m, 1H), 2.93-2.96 (an, 1H), 3.07-3.13 (m, 1H), 3.99-4.03 (m, 1H), 4.13-4.17 (m, 1H), 4.24-4.27 (m, 1H), 4.57-4.61 (m, 3H), 5.03-5.06 (m, 1H), 6.75-6.82 (m, 2H), 7.26 (m, 1H), 7.31-7.37 (m, 2H), 7.76 (m, 1H), 7.94 (m, 1H), 10.40 (m, 1H), 12.48 (m, 1H); ES⁺ MS: 516 (M+1).

EXAMPLE Z-35 racemic-(4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide

-   a)

racemie-(4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-11,18-dioxo-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14-a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide. racemic-cis2-Hydroxymethyl-1 -cyclohexylamine hydrochloride (24 mg, 0.186 mmol) was dissolved in a dichloromethane solution containing a small amount of methanol (to dissolve) and excess MP-Carbonate (Argonaut Technologies) was added, the mixture was stirred for 30 minutes, and the MP-Carbonate was removed by filtration. The free amine solution was transferred to a microwave vessel containing 16a (29 mg, 0.0617 mmol). One drop of glacial acetic acid was added and the solution was heated for 10 minutes at 140° C. The resultant solution was absorbed on celite and the material was purified by silica gel chromatography (0-12% methanol/dichloromethane gradient elution) to yield the desired product as a white solid (1.8 mg, 53%). ¹H NMR (CDCl₃) δ 10.40 (m, 1H), 8.35 (s, 1H), 7.60 (m, 2H), 7.34-7.26 (m, 4H), 6.80 (m, 2H), 5.35-5.23 (m, 2H), 5.13 (m, 1H), 4.77 (m, 1H), 4.70 (m, 2H), 4.22 (dd, J=13.2, 3.2 Hz, 1H), 4.07 (dd, J=13.2, 6.4, 1H), 3.96 (m, 1H), 3.76 (dd, J=11.2, 4.4, 1H), 2.22 (m, 1H), 1.84 (m, 1H), 1.74-1.40 (m, 6H), 1.17 (m, 1H); MS: 550 (M+1).

-   b)

racemic-(4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[3,1]benzoxazine-10-carboxamide.

racemic-(4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide (13 mg, 0.0236 mmol) was dissolved in tetrahydrofuran and 10 w.t. % Pd/C (13 mg) was added. Hydrogen was passed through the solution several times and the mixture was stirred at 1 atm hydrogen for 18 hours until the reaction was determined complete by TLC (5% methanol/dichloromethane). The mixture was filtered through Celtite, eluting with methanol/chloroform and the filtrate was concentrated under reduced pressure and purified by HPLC to yield the title compound (7.3 mg, 73%) ¹H NMR (CDCl₃) δ 12.45 (m, 1H), 10.38 (s, 1H), 8.30 (s, 1H), 7.32 (m, 1H), 6.83-6.76 (m, 2H), 5.23 (m, 1H), 4.75 (m, 1H), 4.63 (m, 2H), 4.26 (m, 1H), 4.12-4.01 (m, 2H), 3.83 (m, 1H), 2.30 (m, 1H), 1.91 (m, 1H), 1.80 (m, 1H), 1.67-1.40 (m, 5H), 1.20 (m, 1H); ES⁺ MS: 460 (M +1).

EXAMPLE Z-36 racamic-(4aR,6aR,14aS)-N-[(4-Fluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a^(,7,11,13,14)a-decahydro-2H-pyridor[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide

-   a)

racemic-(4aR,6aR,14aS)-N-[(4-Fluorophenyl)methyl]-11,13-dioxo-12-[(phenyl methypoxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide. In a manner similar to that described in example Z-35, from racemic-cis-2-Hydroxymethyl-1-cyclohexylamine hydrochloride (50 mg, 0.303 mmol) and 16 (45 mg, 0.0995 mmol) was prepared racemic-(4aR,6aR,14aS)-N-[(4-fluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide (4.8 mg, 91%) as a white solid. ¹H NMR (CDCl₃) δ 10.42 (m, 1H), 8.37 (s, 1H), 7.59 (m, 2H), 7.88-7.24 (m, 5H), 6.98 (m, 2H), 5.26-5.18 (m, 2H), 5.07 (m, 1H), 4.74 (m, 1H), 4.62-4.51(m, 2H), 4.20 (dd, J=18.6, 4 Hz, 1H), 4.04 (m, 1H), 3.91 (m, 1H), 3.71 (dd, J=11.3, 4.8 Hz, 1H), 2.18 (m, 1H), 1.82 (m, 1H), 1.78-1.63 (m, 2H), 1.62-1.56 (m, 2H), 1.48 (1H), 1.38 (m, 1H), 1.14 (m, 1H); ES⁺ MS: 532 (M+1).

-   b)

racemic-(4aR,6aR14aS)-N[(4-Fluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide. In a manner similar to that described in example Z-37, from racemic-(4-aR,6aR,14aS)-N-[(4-fluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide (37 mg, 0.0696 mmol) and 10 w.t. % Pd/C (3 mg) was prepared the title compound (18 mg, 58%) as a white solid after purification by HPLC. ¹H NMR (CDCl₃) δ 12.47 (s, 1H), 10.39 (m, 1H), 8.32 (s, 1H), 7.30 (m, 2H), 6.98 (m, 2H), 5.22 (m, 1H), 4.74. (m, 1H), 4.58 (m, 2H), 4.28 (dd, J=13.2, 4 Hz, 1H), 4.12-3.98 (m, 2H), 3.81 (dd, J=11.6, 4.8 Hz, 1H), 2.29 (m, 1H), 1.91-1.19 (m, 8H); ES⁺ MS: 442 (M+1).

EXAMPLE Z-37 racemic-(3S,4aR,6aR,14aS)-N-[(2 4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-3-phenyl-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide

-   a)

racemic-(3S,4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-11,13-dioxo-3-phenyl-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide. In a manner similar to that described in example Z-35, from racemic-[(1R,2S,5S)-2-amino-5-phenylcyclohexyl]methanol hydrochloride (32 mg, 0.160 mmol) and 16a (30 mg, 0.064 mmol) was prepared racemic-(3S,4aR,6aR,14aS)-N-[(2,4-difluorophenyl)methyl]-11,13-dioxo-3-phenyl-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide (35 mg, 88%) as a white solid. ¹H NMR (CDCl₃) δ 10.41 (m, 1H), 8.38 (s, 1H), 7.66 (m, 2H), 7.40-7.26 (m, 6H), 6.81 (m, 3H), 5.32-5.25 (m, 2H), 5.17 (m, 1H), 4.89 (m, 1H), 4.66-4.62 (m, 2H), 4.26 (dd, J=13.6, 4 Hz, 1H), 4.13-4.04 (m, 2H), 3.85 (dd, J=11.2, 4.4 Hz, 1H), 2.56 (m, 1H), 2.37 (m, 1H), 2.03-1.64 (m, 6H); ES⁺ MS: 626 (M +1).

-   b)

racemic-(3S,4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-3-phenyl-1,3,4,4a,5,6a,7 ,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide.

raceinic-(3S,4aR,6aR,14aS)-N-[(2,4-Difluorophenyl)methyl]-11,13-dioxo-3-phenyl-12-[(phenylmethyl)oxy]-1,3,4,4a,5,6a,7,11,13,14a-decahydro-2H-pyrido[1′,2′:4,5]pyrazino[1,2-a][3,1]benzoxazine-10-carboxamide (27 mg, 0.0432 mmol) was suspended in methanol, 10 w.t, % Pd/C (3 mg) was added and hydrogen was bubbled through the system several times until the reaction was determined complete by TLC (5% methanol/dichloromethane). The suspension was filtered through Celite eluting with methanol/chloroform and the filtrate was concentrated under reduced pressure and purified by HPLC to give the title compound (13 mg, 57%) as a white solid. ¹H NMR (CDCl₃) δ 12.40 (br s, 1H), 10.37 (m, 1H), 8.32 (s, 1H), 7.37-7,28 (m, 3H), 7.24-7.15 (m, 4 H), 6.79 (m, 2H), 5.78 (hr s, 1H), 4.85 (m, 1H), 4.62 (m, 2H), 4.29 (m, 1H), 4.16-4.09 (m, 2H), 3.92 (dd, J=11.6, 4.8 Hz, 1H), 2.58 (m, 1H), 2.46 (m, 1H), 2.07-1.64 (m, 7 H); ES⁺ MS: 536 (M+1).

EXAMPLE Z-38 Sodium racemic-(4aS,6aS,14aS)-10-({[(2,4-difluorophenyl)methyl]amino}carbonyl)-6-(2-methyl propyl)-13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahvdropyrido[1′,2′:4,5]pyrazino[1 2-a]quinazolin-12-olate

-   a) racemic-1,1-Dimethylethyl     [(1S,2R)-2-(hydroxymethyl)cyclohexyl]carbamate,     racemic-[(1R,2S,5S)-2-Amino-5-phenylcyclohexyl]methanol     hydrochloride (800 mg, 4.82 mmol) was dissolved in MeOH (40 mL) and     bis(1,1-dimethylethyl) dicarbonate (1.16 g, 5.30 mmol) and     triethylamine (4 mL, 28.92 mmol) were added and the mixture was     stirred 18 hours at ambient temperature. The solvents were removed     under reduced pressure, ethyl acetate and aqueous saturated sodium     bicarbonate were added and the product was extracted with ethyl     acetate. The combined organics were dried over sodium sulfate and     the solvents were removed under reduced pressure. Purification by     silica gel chromatography (9:1 hexane:ethyl acetate to ethyl acetate     gradient elution) gave 1,1-dimethylethyl     racemic-[(1S,2R)-2-(hydroxymethyl)cyclohexyl]carbamate (934 mg, 85%)     as a white solid. ¹H NMR (CDCl₃) δ 4.87 (m, 1H), 4.03-3.95 (m, 2H),     3.26 (m, 1. H), 3.15 (m, 1H), 1.73-1.48 (m, 5H), 1.38 (s, 9H).     1.27-1.15 (m, 3H), 0.887 (m, 1H). -   b) racemic-1,1-Dimethylethyl [(1S,2R)-2-Formylcyclohexyl]carbamate.     To a solution of dimethylsulfoxide (0.2 mL, 2.88 mmol) in     dichloromethane (3 mL) at −78° C. was added oxalyl chloride (0.72     mL, 1.44 mmol) dropwise. The mixture was stirred 10 minutes and     racemic-1,1-dimethylethyl     [(1S,2R)-2-(hydroxymethyl)cyclohexyl]carbamate (220 mg, 0.961 mmol)     in dichlormethane was added dropwise and stirred 10 minutes,     Triethylamine (0.53 mL, 3.84 mmol) was added slowly and the reaction     was stirred at −78° C. for one hour and allowed to warm to ambient     temperature. Water was added and product was extracted with     dichloromethane. The combined organics were washed with brine and     dried over sodium sulfate. Removal of solvents under reduced     pressure afforded racemic-1,1-dimethylethyl     [(1S,2R)-2-formylcyclohexyl]carbamate (223 mg, quantitative) as a     yellow oil. ¹H NMR (CDCl₃) δ 9.61 (s, 1H), 5.19 (m, 1H), 3.88 (m,     1H), 2.61 (m, 1H), 1.85 (m, 1H), 1.63-1.49 (m, 4H), 1.37-1.16 (m,     12H). -   c) racemic-1,1-dimethylethyl     ((1S,2S)-2-{[(2-Methylpropyl)amino]methyl}cyclohexyl)carbamate,     racemic-1,1-Dimethylethyl [(1S,2R)-2-formylcyclohexyl]carbamate (223     mg, 0.982 mmol) was dissolved in dichloroethane and     2-methylpropyl)amine (0.15 mL, 1.47 mmol) and sodium     triacetoxyborohydride (290 mg, 1.37 mmol) were added and the     reaction was stirred at ambient temperature for 18 hours. Aqueous     sodium bicarbonate was added and the product was extracted with     dichloromethane. The combined extracts were dried over sodium     sulfate and the solvents were removed under reduced pressure.     Purification by silica gel chromatography (dichloromethane to 1%     ammonium hydroxide 19% methanol 80% dichloromethane gradient     elution) afforded racemic-1,1-dimethylethyl     ((1S,2S)-2-{[(2-methylpropyl)amino]methyl}cyclohexyl)carbamate (112     mg, 40%) as a clear colorless oil. ¹H NMR (CDCl₃) δ 6.06 (br s, 1H),     3.76 (br s, 1H), 2.63 (m, 1H), 2.43-2.37 (m, 2H), 2.25 (m, 1H), 1.81     (m, 1H), 1.71-1.59 (m, 3H), 1.44-1.32 (m, 14H), 1.27-1.19 (m, 2H),     0.866 (m, 6H). -   d) racemic-(1S,2S)-2-{[(2-Methylpropyl)amino]methyl}cyclobexanamine     hydrochloride.

In a manner similar to that describe in example Z-3, step e, from racemic-1,1-dimethylethyl ((1S,2S)-2-{[(2-methylpropyl)amino]methyl}cyclohexyl)carbamate (112 mg, 0.394 mmol) was prepared (1S,2S)-2-({[(2-methylpropyl)amino]methyl}cyclohexanamine hydrochloride (180 mg, >100%) as a white solid. ¹H NMR (methanol-d₄/CDCl₃) δ 8.68-8.28 (m, 1H), 3.62 (br s, 1H), 3.26 (m, 1H), 2.83-2.78 (m, 3H), 2.54 (br s, 1H), 2.12 (m, 1H), 1.82-1.66 (m, 3H), 1.53-1.39 (m, 5H), 0.96 (m, 6H), 0.766 (m, 1H).

-   e)

racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-6-(2-methylpropyl)-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in Z-35, from racemic-(1S, 2S)-2-{[(2-methyl}propyl)amino]methyl)cyclohexanamine hydrochloride (130 mg, 0.508 mmol) and 16a (55 mg, 0.117 mmol) was prepared racemic-(4aS,6aS,14aS)-N[(2,4-difluorophonyl)methyl]-6-(2-methylpropyl)-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyride[1′,2′:4,5]pyrazino[1,2a]quinazoline-10-carboxamide (44 mg, 62%) with a 12:1 d.r. ¹H NMR (CDCl₃) δ 10.46 (m, 1H), 8.33 (s, 1H), 7.59 (m, 2H), 7.37-7.24 (m, 4 H), 6.79 (m, 2H), 5.30-5.23 (m, 2H), 4.75-4.56 (m, 3H), 4.23-4.09 (m, 3H), 2.69-2.66 (m, 2H), 2.21-1.98 (m, 3H), 1.80 (m, 1H), 1.71-1.33 (m, 6H), 1.26-1.19 (m, 2H), 0.810 (m, 3H), 0.720 (m, 3H); MS: 605 (M+1).

-   f)

racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-(2-methylpropyl)-11,13-dioxo-1,2, 3,4,4a,5,6, 6a,7,11,13,14a-clodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamicle. In a manner similar to that described in example Z-37, from racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-(2-methylpropyl)-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10carboxamide (39 mg, 0.064 mmol) and 10 w.t. % Pd/C (7 mg) was prepared racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-6-(2-methylpropyl)-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1,′2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (36 mg, >100%) as a tan solid. ¹H NMR, (CDCl₃) δ 12.60 (br s, 1H), 10.43 (br s, 1H), 8.25 (s, 1H), 7.35 (m, 1H), 6.78 (m, 2H), 4.77 (m, 1H), 4.63 (m, 2H), 4.49 (br s, 1H), 4.30-4.13 (m, 2H), 3.63-3.40 (m, 2H), 2.88-2.71 (m, 2H), 2.32-2.21 (m, 2H), 2.05 (m, 1H), 1.88-1.11 (m, 7H), 0.830 (m, 3 H), 0.760 (m, 3H); AP⁺ MS: 515 (M +1).

-   g) Sodium     racemic-(4aS,6aS,14aS)-10-({[(2,4-Difluorophenyl)methyl]amino}carbonyl)-6-(2-methyl     propyl)-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyricio[1,′2′:4,5]pyrazino[1,2-a]quinazolin-12-olate.     In a manner similar to that described in example Z-1, from     racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-6-(2-methylpropyl)-11,13-dioxo-1,2,3,4,4a5,6,6a,7,11,13,14a-doclecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide     (37 mg, 0.071 mmol) and 1 N sodium hydroxide (0.07 mL) the title     compound was prepared as a yellow solid (26 mg, 68%). ¹H NMR     (DMSO-d₆) δ 10.73 (m, 1H), 7.94 (s, 1H), 7.32 (m, 1H), 7.19 (m, 1H),     7.00 (m, 1H), 4.59-4.41 (m, 3 H), 4.28 (m, 2H), 4.14 (br s, 1H),     2.63-2.60 (m, 2H), 1.98-1.61 (m, 5H), 1.48-1.36 (m, 4H), 0.997 (m,     3H), 0.760 (m, 3H), 0.660 (m, 2H); AP⁺ MS: 515 (M+1 of free acid).

EXAMPLE Z39 (6aR,7aS,11aS)-N-[(2,4-Difluorophenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide & Example Z-40 (6aS,7aS,11aS)-N-[(2,4-Difluoroohenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,7 a ,8,9,10,11,11a,13-deoahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide

-   a)

(6aR,7aS,11aS)-N-[(2,4-Difluorophenyl)methyl]-2,13-dioxo-1[(phenylmethyl)oxyl]-2,6a,7,7a,8,9,10,11,11a,18-decahydro-6H-pyrido [1′,2′:4,5] pyrazino[1,2-a]benzimiclazole-3-carboxamide and (6aS,7aS,11aS)-N-[(2,4-difluorophenyl)methyl]-2,13-dioxo-1-[(phenylmethyl)oxy]-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide. In a manner similar to that described in example Z-2, from [(1S,2S)-2-aminocyclohexyl]amine (122 mg, 1.07 mmol) and 16a (200 mg, 0.426 mmol) was prepared (6aR,7aS,11aS)-N-[(2,4-difluorophonyl)methyl]-2,13-dioxo-1-[(phenylmethyl)oxy]-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido [1′,2′:4,5] pyrazino[1,2-a]benzimidazole-3-carboxamide (58 mg) and (6 aS,7aS,11aS)-N-[(2,4-difluorophenyl)methyl]-2,13-dioxo-1-[(phenylmethyl)oxy]-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide (10.6 mg) after separation of the diastereorners using silica gel chromatography (0-12% methanol/dichloromethane). (6aR,7aS,11aS)-N-[(2,4-difluorophenyl)methyl]-2,13-dioxo-1-[(phenylmethyl)oxy]-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido [1′,2′:4,5] pyrazino[1,2-a]benzimidazole-3-carboxamide (major): ¹H NMR (CDCl₃) δ 10.40 (m, 1H), 8.33 (s, 1H), 7.57 (m, 2H), 7.40-7.25 (m, 4H), 6.81 (m, 2H), 5.32 (d, J=10 Hz, 1H), 5.13 (d, J=10 Hz, 1H), 4.64-4.58 (m, 3H), 4.21 (dd, J=12.4, 3.2 Hz, 1H), 3.79 (m, 1H), 3.04 (m, 1H), 2.73 (m, 1H), 2.53 (m, 1H), 2.01-1.79 (m, 4H), 1.36-1.24 (m, 4H); ES⁺ MS: 535 (M+1). (6aS,7aS,11aS)-N-[(2,4-difluorophenyl)methyl]-2,13-dioxo-1-[(phenylmethyl)oxy]-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide (minor diastereomer): ¹H NMR (CDCl₃) δ 10.33 (m, 1H), 8.28 (s, 1H), 7.61 (m, 2H), 7.39-7.28 (m, 3H), 6.79 (m, 2H), 5.29 (d, J=9.6 Hz, 1H), 5.05 (d, J=9.6 Hz, 1H), 4.84 (m, 1H), 4.60 (m, 2H), 3.90-3.84 (m, 2H), 3,07 (m, 1H), 2.75 (m, 1H), 2.49 (m, 1H), 2.07 (m, 1H), 1.90-1.51 (m, 4H), 1.33-1.19 (m, 4H); MS data matches that of its diastereomor.

-   b) (For example Z-39),     (6aR,7aS,11aS)-N-[(2,4-Difluorophenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,7a,8,9,10,11,11a,13-docahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide.     In a manner similar to that described in example Z-37, from the     minor diastereomer prepared in step a     (6aS,7aS,11aS)-N-[(2,4-difluorophenyl)methyl]-2,13-dioxo-1-[(phenylm     ethyl)oxy]-2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide     (7 mg, 0.0131 mmol) and 10 w.t. % Pd/C (catalytic amount) was     prepared     (6aR,7aS,11aS)-N-[(2,4-difluorophenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,     7a,8,9,-10,11,11a,13-docahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide     (2.8 mg, 48%) after purification by HPLC. NMR (CDCl₃) δ 12.15 (br s,     1H), 10.42 (br s, 1H), 8.31 (s, 1H), 7.36 (m, 1H), 6.80 (m, 2H),     5.01 (m, 1H), 4.63 (m, 2H), 4.16 (m, 1H), 3.96 (m, 1H), 3.06-2.93     (m, 2H), 2.61 (m, 1H), 2.18 (m, 1H), 1.93 (m, 1H), 1.60-1.13 (m,     4H), 0.893-0.840 (m, 2H); ES⁺ MS: 44.5 (M +1). -   c) (For example Z-40).     (6aS,7aS,11aS)-N-[(2,4Difluorophenyl)methyl]-1-hydroxy-2,13-dioxo-2,6a,7,7     a,8,9,10,11,1,1a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide.     In a manner similar to that described in example Z-37, from the     major diastereomer (30 mg, 0.0561 mmol) prepared in step a and 10     w.t. % Pd/C (catalytic amount),     (6aS,7aS,11aS)-N-[(2,4-Difluorophenyl)methyl]-1-hydroxy-2,13-dioxo2,6a,7,7a,8,9,10,11,11a,13-decahydro-6H-pyrido[1′,2′:4,5]pyrazino[1,2-a]benzimidazole-3-carboxamide     was prepared as a white solid (15 mg, 60%) after purification by     HPLC. ¹H NMR (methanol-d₄/CDCl₃) δ 10.41 (m, 1H), 8.25 (s, 1H), 7.30     (m, 1H), 6.77 (m, 2H), 4.77 (m, 1H), 4.57 (m, 2H), 4.45 (m, 1H),     3.91 (m, 1H), 3.12 (m, 1H), 2.67 (m, 1H), 2.12 (m, 1H), 1.87-1.84     (m, 2H), 1.47-1.33 (m, 4H); ES⁺ MS: 44.5 (M+1).

EXAMPLE Z-41 (5aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyridr[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide

-   a)

(5aS,14aS)N-[(2,4-Difluorophcnyl)methyl]-10,12-dioxo-11-[(phenylmethyl)oxy]-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide. In a manner similar to that described in example Z-18, from 16a (50 mg, 0.108 mmol) and [(2S)-2-piperidinylmethyl]amine hydrochloride (50 mg, 0.289 mmol, made in a similar manner as described in example Z-18) was prepared (5aS,14as)-N-[(2,4-difluorophenyl)methyl]-10,12-dioxo-11-[(phenylmethyl)oxy]-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide (4.0 mg, 78%). ¹H NMR (CDCl₃) δ 10.43 (m, 1H), 8.38 (s, 1.H), 7.59 (m, 2H), 7.59-7.25 (m, 4H), 0.81 (m, 2H), 5.38 (d, J=10 Hz, 1H), 5.19 (d, J=10 Hz, 1H), 4.65-4.62 (m, 2H), 4.20 (dd, J=12, 2.8 Hz, 1H), 4.00 (dd, J=12.4, 2.8 Hz, 1H), 3.85 (m, 1H), 3.74 (m, 1H), 3.27 (m, 1H), 2.99 (m, 1H), 2.43 (m, 1H), 2.24 (m, 1H), 1.94-1.87 (m, 2H), 1.77-1.58 (m, 2H), 1.39-1.24 (m, 2H); LS⁺ MS: 535 (M +1).

-   b)

(5aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide.

In a manner similar to that described in example Z-37, from (5aS,14aS)-N-[(2,4-difluorophenyl)methyl]-10,12-dioxo-11-[(phenylmethyl)oxy]-1,2,3,4,5a,6,10,12,14,14a-decahydropyrido[1,2-a]pyrido[1′,2′:3,4]imidazo[1,2-d]pyrazine-9-carboxamide (18 mg, 0.0337 mmol) and 1.0 w.t. % Pd/C (catalytic amount) was prepared the title compound as a white solid (13 mg, 87%) after purification by HPLC, ¹H NMR (CDCl₃) δ 11.71 (br s, 1H), 10.36 (br s, 1H), 8.31 (s, 1H), 7.34 (m, 1H), 6.78 (m, 2H), 4.64-4.57 (m, 2H), 4.28 (m, 1H), 4.12 (m, 1H), 3.92-3,89 (m, 2H), 3.22 (m, 1H), 3.04. (m, 1H), 2.49 (m, 3H), 2.28 (m, 1H), 1.97-1.89 (m, 2H), 1.78 (m, 1H), 1.66-1.60 (m, 2H), 1.43-1.36 (m, 2H); ES⁺ MS: 445 (M+1).

EXAMPLE Z-42 (4aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-9-hydroxy-8,10-dioxo-2,3,4,4a,5,6,8,10,14,14a -decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide

-   a) Phenylmethyl (2R)-2-(hydroxymethyl)-1-piperidinecarboxylate. In a     manner similar to that described in example Z-3a, from     (2R)-1-{[(phenylmethyl)oxy]carbonyl}-2-piperidinecarboxylic acid     (4.93 g, 18.75 mmol) was prepared phenylmethyl     (2R)-2-(hydroxymethyl)-1-piperidinecarboxylate (2.24 g, 48%) as an     oil that solidified upon standing to a white solid. ¹H NMR (CDCl₃) δ     7.36-7.26 (m, 5H), 5.18-5.10 (m, 2H), 4.37 (m, 1H), 4.03 (m, 1H),     3.84 (m, 1H), 3.63 (m, 1H), 2.96 (br s, 1H), 1.71-1.42 (m, 6H). -   b) Phenylmethyl (2R)-2-(cyanomethyl)-1-piperidinecarboxylate. In a     manner similar to that described in example Z-3b, from phenylmethyl     (2R)-2-(hydroxymethyl)-1-piperidinecarboxylate (1.09 g, 4.38 mmol)     was prepared phenylmethyl     (2R)-2-({[(4-methylphenyl)sulfonyl]oxy}methyl)-1-piperidinecarboxylate     (1.05 g, 59% impure with uncharacterized byproduct) as a clear     colorless oil after purification using silica gel chromatography     (10-100% ethyl acetatehexanes). It is necessary to use this material     in the next step as soon as possible or yields deteriorate     dramatically, in a manner similar to that described in example Z-3c,     from phenylmethyl     (2R)-2-({[(4-methylphenyl)sulfonyl]oxy}methyl)-1-piperidinecarboxylate     (1.05 g, 2.61 mmol) and sodium cyanide (383 mg, 7.82 mmol) was     prepared phenylmethyl (2R)-2-(cyanomethyl)-1-piperidinecarboxylate     (171 mg, 25%) as a yellow oil. ¹H NMR (CDCl₃) δ 7.35-7.29 (m, 5H),     5.13 (s, 2H), 4.65 (m, 1H), 4.10 (m, 1H), 2.96 (m, 1H), 2.60 (m,     2H), 1.,82-1.67 (m, 4H), 1.54-1.39 (m, 2H). -   d) Phenylmethyl (2R)-2-(2-aminoethyl)-1-piperidinecarboxylate. In a     manner similar to that described in example Z-3d, from phenylmethyl     (2R)-2-(cyanomethyl)-1-piperidinecarboxylate (171 mg, 0.663 mmol)     was prepared phenylmethyl     (2R)-2-(2-aminoethyl)-1-piperidinecarboxylate (119 mg, 68%) as a     clear colorless residue. ¹H NMR (CDCl₃) δ 7.32-7.25 (m, 5H), 5.08     (m, 2H), 4.39 (br s, 1H), 4.01 (br s, 1H), 2.78 (m, 1H), 2.60-2.56     (m, 2H), 1.95-1.86 (m, 3H), 1.63-1.35 (m, 6 H). -   e) {2-[(2R)-2-Piperidinyl]ethyl}amine. Phenylmethyl     (2R)-2-(2-aminoethyl)-1-piperidinecarboxylate (119 mg, 0.454 mmol)     was dissolved in methanol and 10 w.t. % Pd/C (120 mg) was added,     Hydrogen was bubbled through the solution for 15 minutes and the     reaction was stirred under 1 atm hydrogen for 18 hours until     determined complete by TLC (1% ammonium hydroxide 19% methanol 80%     dichloromethane). The suspension was filtered through Celite eluting     with methanol and the filtrate was carefully concentrated under     reduce pressure to yield a clear colorless liquid (58 mg,     quantitative). ¹H NMR (CDCl₃) δ 2.99 (m, 1H), 2.71-2.66 (m, 2H),     2.57-2.48 (m, 2H), 1.72 (m, 1H), 1.61-1.52 (m, 2H), 1.48-1.42 (m,     2H), 1.35-1.25 (m, 2H), 1.05 (m, 1H). -   f)

(4aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-8,10-dioxo-9-[(phenylmethyl)oxy]-2,3,4,4a,5,6,8,10,14,14a-decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide. In a manner similar to that described in example Z-35, from 16a (50 mg, 0.106 mmol) and {2-[(2R)-2-piperidinyl]ethyl}amine (58 mg, 0.454 mmol) was prepared (4aR,14aR)-N-[(2,4-difluorophenyl)methyl]-8,10-dioxo-0-[(phenylmethyl)oxy]-2,3,4,4a,5,6,8,10,14,14a-docahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide (47 mg, 81%), ¹H NMR (CDCl₃) δ 10.50 (br s, 1H), 8.33 (s, 1H), 7.60 (s, 2H), 7.38-7.24 (m, 4H), .,80 (m, 2H), 5.29-5.22 (m, 2H), 4.66-4.56 (m, 3H), 4.30 (m, 1H), 4.19 (m, 1H), 3.78 (br s, 1H), 2.86-2.80 (m, 2H), 2.18 (br s, 1H), 1.94 (m, 1H), 1.68-1.36 (m, 6H), 1.23 (br s, 2H); ES⁺ MS: 549 (M+1).

-   g)

(4aR,14aR)-N-[(2,4-Difluorophenyl)methyl]-9-hydroxy-8,10-dioxo-2,3,4,4a,5,6,8,10,14,14a-decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide. In a manner similar to that described in example Z-37, from (4aR,14aR)-N-[(2,4difluorophenyl)methyl]-8,10-dioxo-9-[(phenylmethyl)oxy]-2,3,4,4a,5,6,8,10,14,14a-decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide (47 mg, 0.0857 mmol) and a catalytic amount of 10 w.t. % Pd/C was prepared the title compound as a white solid (19 mg, 54%) after purification by HPLC. ¹H NMR (CDCl₃) δ 10.49 (m, 1H), 8.29 (s, 1H), 7.34 (m, 1H), 6.79 (m, 2H), 4.67-4.56 (m, 3H), 4.41 (m, 1H), 4.20 (m, 1H), 3.93 (s, 1H), 2.94-2.87 (m, 2 H), 2.28 (br s, 1H), 2.01 (m, 1H), 1.68-1.54 (m, 4H), 1.44 (m, 1H), 1.29-1.23 (m, 3H), 0.850 (m, 1H); ES⁺ MS: 459 (M+1).

EXAMPLE Z-43 (4R,12aR)-N-[(2,4Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(8-methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3R)-3-Ainobutyl](3-methylbutyl)amine dihydrochloride was     prepared in a similar manner as described in example Z-32. ¹H NMR     (400 MHz, CDCl₃/CD₃OD) δ 0.87 (d, J=5.2 Hz, 6H), 1.32 (m, 3H), 1.61     (m, 3H), 2.10-2.20 (m, 2H), 2.90-3.04 (m, 4H), 3.45 (m, 1H), 8.23     (br, <1H.), 8.96 (br, <1H). -   b)

(4R,12aR)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(3methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps rising a similar process to that described in example Z-2, 16a (40 mg, 0.085 mmol) and free [(3R)-3-aminobutyl](3-methylbutypamine (46 mg, 0.35 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4R,12aR)-N-[(2,4-difluorophenyl)methyl]-4-methyl-1-(3-methylbutyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (44 mg, 90%) as a film, This material was hydrogenated in a second step as described in example Z-2 to give (4R,12aR)-N-[(2,4-difluorophenyl)methyl]7-hydroxy-4-methyl-1-(3-methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1,′2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (11 mg, 30%) as an off-white solid, ¹H NMR (400 MHz, CDCl₃) δ 0.84 (d, J=6.8 Hz, 3H), 0.86 (d, J=6.8 Hz, 3H), 1.24-1.36 (m, 5H), 1.47-1.53 (m, 2H), 2.02-2.11 (m, 1H), 2.36-2.43 (m, 1H), 2.54-2.61 (m, 1H), 2.77-2.92 (m, 2H), 4.16-4.26 (m, 2H), 4.44 (m, 1H), 4.62-4.64 (m, 2H), 4.95-5.02 (m, 1H), 6.75-6.81 (m, 2H), 7.31-7.37 (m, 1H), 8.27 (s, 1H), 10.43 (mi, 1H), 12.54 (s, 1H); ES⁺ MS: 489 (M+1).

EXAMPLE Z-44 (4S,12aS)-N-[(2,4-Difluorophenpl)methyl]-7-hydroxv-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine9-carboxamide

-   a) [(3S)-8-Aminobutyl](1-methylethyl)amine dihydrochloride was     prepared in a similar manner as described in example Z-29, ¹H NMR     (400 MHz, CDCl₃/CD₃OD) δ 1.20-1.25 (m, 9H), 1.93-2.02 (m, 2H), 2.92     (m, 2H), 3.20-3.29 (m, 2H), 8.04 (br, <1H), 8.64 (br, <1H). -   b)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylothyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2, 16a (60 mg, 0.13 mmol) and free based [(3S)-3-aminobutyl](1-methylethyl)amine (55 mg, 0.42 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S, 12aS)-N-[(2,4-difluorophenyl)methyl]-4-methyl-1-(1-methylethyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine 9-carboxamide (40 mg, 57%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (17 mg, 50%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.02 (d, J=6.4 Hz, 3H), 1.07 (d, J=6.4 Hz, 3H), 1.33 (d, J=7.2 Hz, 3H), 1.55-1,58 (m, 1H), 1.94-2.03 (m, 1H), 2.70-2.77 (m, 1H), 2.81-2.86 (m, 1H), 3.11-3.18 (m, 1H), 4.17 (dd, J=3.0, 13.8 Hz, 1H), 4.32 (dd, J=3.2, 14.0 Hz, 1H), 4.48 (m, 1H), 4.59-4.69 (m, 2H), 4.97-5.00 (m, 1H), 6.77-6.83 (m, 2H), 7.33-7.39 (m, 1H), 8.28 (s, 1H), 10.50 (m, 1H), 12.55 (s, 1H); ES⁺ MS: 461 (M+1).

EXAMPLE Z-45 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(3-methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl](3-methylbutyl)amine dihydrochleride was     prepared in a similar manner as described in example Z-32. ¹H NMR     (400 MHz, CDCl₃/CD₃OD) δ 0.86 (d, J=5.6 Hz, 6H), 1.27 (d, J=6.0 Hz,     3H), 1.58 (m, 3H), 2.03-2.14 (m, 2H), 2.87-2.09 (m, 4H), 3.38 (m,     1H), 8.15 (br, <1H), 8.87 (br, <1H). -   b)

(4S,12aS)-N-[(2,4-difluorophenyl)methy]-7-hydroxy-4-methyl-1-(3-methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (0.100 g, 0.21 mmol) and free based [(3S)-3-aminobutyl](3-methylbutyl)amine (0.104 g, 0.66 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophonyl)methyl]-4-methyl-1-(3-methylbutyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (88 mg, 72%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-1-(3-methylbutyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (55 mg, 74%). ¹H NMR (400 MHz, CDCl₃) δ 0.84 (d, J=6.4 Hz, 3H), 0.85.(d, J=6.4 Hz, 3H), 1.24-1.37 (m, 5H), 1.45-1.53 (m, 2H), 2.02-2.11 (m, 1H), 2.37-2.44 (m, 1H), 2.56-2.63 (m, 1H), 2.80-2.92 (m, 2H), 4.22-4.29 (m, 2H), 4.45 (s, 1H), 4.62-4.63 (m, 2H), 4.97-5.00 (m, 1H), 6.75-6.82 (m, 2H), 7.31-7.37 (m, 1H), 8.37 (s, 1H), 10.48 (m, 1H), 12.53 (br, 1H); ES⁺ MS: 489 (M+1).

EXAMPLE Z-46 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4 -methyl-6,8-dioxo-1 -(3-pyridinyl methyl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) 1,1-Dimethylethyl     {(1S)-1-methyl-3-[(3-pyridinylmethyl)amino]propyl}carbamate. The     protected diamine was prepared using a modified procedure as     described in example Z-32. A solution of 1,1-dimethylethyl     [(1S)-3-amino-1-methylpropyl]carbamate (0.296 g, 1.6 mmol) and     3-pyridinecarboxaldehyde (120 μL, 1.3 mmol) in a 1:1 mixture of     anhydrous dichloroethane and tetrahydrofuran (10 mL) was treated     with acetic acid (374 μL, 6.6 mmol) and stirred for 80 minutes.     Sodium triacetoxyborohydride (0,444 g, 2.1 mmol) was added and the     solution was stirred for 2 hours. The resultant was subjected to a     workup and purification procedure as described in example Z-32 to     give 1,1-dimethylethyl     {(1S)-1-methyl-3-[(8-pyridinylmethyl)amino]propyl}carbamate (0.245     g, 66%) as a clear oil. ¹H NMR (400 MHz, CDCl₃) δ 1.12 (d, J=6.4 Hz,     3H), 1.42 (s, 9H), 1.46-1.54 (m, 1H), 1.68 (m, 1H), 2.61-2.75 (m,     2H), 3.73-3.80 (m, 4.86 (m, 1H), 7.22-7.24 (m, 1H), 7.68 (d, J=8.0     Hz, 1H), 8.48 (m, 1H), 8.53 (m, 1H). -   b) [(3S)-3-Aminobutyl](3-pyridinylmethyl)amine dihydrochloride was     prepared in a similar manner as described in example Z-29. -   c)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(3-pyridinyl methyl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (60 mg, 0.13 mmol) and free based [(3S)-3-aminobutyl](3-pyridinylmethyl)amine (83 mg, 0.47 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1-(3-pyridinylmethyl)-1,2,3,4,6,8,12,12a-octabydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (72 mg, 95%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(3-pyridinyl methyl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (34 mg, 56%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.37 (d, J=6.8 Hz, 8H), 1.43-1.47 (m, 1H), 2.12 (m, 1H), 2.60-2.92 (m, 2H), 3.53 (d, J=14.0 Hz, 1H). 3.82 (d, J=14.4 Hz, 1H), 4.23-4.31 (m, 2H), 4.55-4.64 (m, 3H), 6.06-5.11 (m, 1H), 6.75-6.82 (m, 2H), 7.20-7,28 (m, 1H), 7.31-7.36 (m, 1H), 7,50 (m, 1H), 7.92 (s, 1.H), 8.48 (s, 1H), 10.39 (m, 1H), 12.5 (br, 1H); ES⁺ MS; 510 (M+1).

EXAMPLE Z-47 (4S,12aS)-1-Cyclopropyl-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) 1,1-Dimethylethyl [(1S)-1-methyl-3-oxopropyl]carbamate. To a     stirred solution of 1,1-dimethylethyl     [(1S)-2-cyano-1-methylethyl]carbamate (0.656 g, 3.56 mmol) in     anhydrous ether cooled to −40° C., was added clropwise a 1.0 M     solution of diisobutylaluminum hydride in hexanes (14.2 mL, 14.2     mmol) over 20 minutes. Stirring was continued at this temperature     for an additional 20 minutes. The yellow solution was quenched with     Rochelle's salt and the resultant stirred at room temperature for 1     hour. The solids were filtered off through celite and rinsed with     EtOAc. The organics were washed with brine, concentrated, and flash     chromatographed (10-100% EtOAc/hexanes) to give 1,1-dimethylethyl     [(1S)-1-methyl-3-oxopropyl]carbarnate (0.193 g, 30%) as a clear oil.     ¹H NMR (400 MHz, CDCl₃) δ 1.22 (d, J=6.8 Hz, 3H), 1.41 (s, 9H),     2.58-2.65 (m, 2H), 4.08-4.13 (m, 1H), 4.63 (m, 1H), 9.74-9.75 (m,     1H). -   b) 1,1-Dimethylethyl     [(1S)-3-(cyclopropylamino)-1-methylpropyl]carbamate. The protected     diamine was prepared using a modified procedure as described in     example Z-32. A solution of 1,1-dimethylethyl     [(1S)-1-methyl-3-oxopropyl]carbamate (0.178 g, 0.95 mmol) and     cyclopropylamine (197 μL, 2.85 mmol) in anhydrous dichloroethane (10     mL) was treated with acetic acid (272 μL, 4.8 mmol) and stirred for     30 minutes. Sodium triacetoxyborohydride (0.444 g, 2.1 mmol) was     added and the solution was stirred for 20 hours. The resultant was     subjected to a workup and purification procedure as described in     example Z-32 to give 1,1-dimethylethyl     [(1S)-3-(cyclopropylamino)-1-methylpropyl]carbamatc (0.186 g, 63%)     as a clear oil. ¹H NMR (400 MHz, CDCl₃) δ 0.32-0.42 (m, 4H), 1.12     (d, J=6.8 Hz, 3H), 1.89-1.51 (m, 10H), 1.58-1.92 (m, 2H), 2.05-2.10     (m, 1H), 2.67-2.80 (m, 2H), 3.71 (m, 1H), 4.78 (m, 1H). -   c) [(3S)-3-Aminobutyl]cyclopropylamine dihydrochloride was prepared     in a similar manner as described in example Z-20. ¹H NMR (400 MHz,     CDCl₃/CD₃OD) δ 0.70-0.75 (m, 2H), 0.90-0.94 (m, 2H), 1.18 (d, J=6.8     Hz, 3H), 1.84-1.94 (m, 1H), 1.97-2.05 (m, 1H), 2.49-2.54 (m, 1H),     2.99-3.04 (m, 2H), 3.23-3.28 (m, 1H). -   d)

(4S,12aS)-1-Cyclopropyl-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2. 16a (80 mg, 0.17 mmol) and free based [(3S)-3-aminobutyl]cyclopropylamine (75 mg, 0.59 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-1-cyclopropyl-N-[(2,4-difluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenyl methyl)oxy]1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (74 mg, 80%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-1-cyclopropyl-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (32 mg, 52%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 0.37-0.54 (m, 3H), 0.64-0.70 (m, 1H), 1.35 (d, J=7.2 Hz, 3H), 1.45-1.49 (m, 1H), 1.76-1.80 (m, 1H), 2.03-2.12 (m, 1H), 2.80-2.93 (m, 1H), 2.99-3.04 (m, 1H), 4.30 (dd, J=4.0, 13,6 Hz, 1H); 4.49-4.67 (m, 4H), 5.00-5.07 (m, 1H), 6.75-6.82 (m, 2H), 7.32-7.36 (m, 1.H), 8.28 (s, 1H), 10.49 (m, 1H), 12.53 (s, 1H); ES⁺ MS; 459 (M+1).

EXAMPLE Z-48 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hvdroxy-4methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12 12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl][2-(methyloxy)ethyl]amine dihydrochloride. The     protected diamine, 1,1-dimethylethyl     ((1S)-1-methyl-3-{[2-(methyloxy)ethyl]amino}propyl)carbamate was     prepared in a similar manner as described in example Z47.     Subsequently, [(3S)-3-aminobutyl][2-(methyloxy)ethyl]amine     dihydrochloride was prepared in a similar manner as described in     example Z-29. ¹H NMR (400 MHz, CDCl₃/CD₃OD) δ 1.21 (d, J=5.6 Hz,     3H), 1.93 (m, 1H), 2.04 (m, 1H), 2.98-3.05 (m, 4H), 3.22 (m, 2H),     3.26-3.31 (m, 4H), 8.06 (br, <1H), 8.81 (br, <1H). -   b)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2, 16a (60 mg, 0.13 mmol) and free based [(3S)-3-aminobutyl][2-(methyloxy)ethyllamine (53 mg, 0.37 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (47 mg, 63%) as a film. This material was hydrogenated in a second step as described in example Z2 to give (4S,12aS)-N-[(2,4-difluorophonyl)methyl]-7-hydroxy-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido(1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (38 mg, 97%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.34 (d, J=7.2 Hz, 3H), 1.49 (m, 1H), 2.03-2.12 (m, 1H), 2.67-2.70 (m, 1H), 2.81-2.92 (m, 2H), 3.06-3.15 (m, 1H), 3.30-3.37 (m, 4H), 3.58-3.63 (m, 1H), 4.20 (dd, J=-3.4, 14.2 Hz, 1H), 4.50-4.59 (m, 1H), 4.62-4.65 (m, 3H), 5.00-5.03 (m, 1H), 6.75-6.81 (m, 2H), 7.31-7.37 (m, 1H), 8.27 (s, 1H), 10.46 (s, 1H), 12.54 (s, 1H); ES⁺ MS; 477 (M+1).

EXAMPLE Z-49 racemic-(3aS,5aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-5-(2-methylpropyl)-10,12-dioxo-2,3,3a4,5,5a6,10,12,13a-decahydro-1H-cyclopenta[e]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) racemic-(1S,2S)-2-{[(2-Methylpropyl)amino]methyl}cyclopentanamine     hydrochloride.

In a manner to example Z-18a-c, from racemic-{(1R,2S)-2-[(1,1-dimethylethyl)oxy]carbonyl}amino)cyclopentanecarboxylic acid (255 mg, 1.11 mmol) was vrep a red racemic-1,1-dimethylethyl [(1S,2S)-2-(aminomethyl)cyclopentyl]carbamate (153 mg, 64% over 3 steps) as a white green residue. Reductive amination with isobutyraldehyde followed by deprotection as described in Z-38 steps c and d respectively, gave racemic-(1S,2S)-2-{[(2-methylpropyl)amino]methyl}cyclopentanamine hydrochloride (105 mg, 39% over 5 steps from amino acid). ¹H NMR (methanol-d₄/CDCl₃) 8.90 (br s, <1H), 8.64 (br s, <1H), 8.28 (m, 1H), 3.97 (br s, 1H), 3.37 (m, 1H), 2.83-2.69 (m, 3H), 2.18-1.69 (m, 7H), 0.996 (m, 6H).

-   b)

raceinic-(3aS,5aS,13aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-5-(2-methylpropyl)-10,12-dioxo-2,3,3a,4,5,5a,6,10,12,13a-decahydro1H-cyclopenta[e]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrirraidine-9-carboxamide. In a manner similar to that described example Z-35, from racemic-(1S,2S)-2-[(2-methylpropyl)amino]methyl)cyclopentanamine hydrochloride 1.05 mg, 0.434 mmol) and 16a (56 mg, 0.119 mmol) was prepared racemic-(3aS,5aS,13aS)-N-[(2,4-difluorophenyl)methyl]-5-(2-methylpropyl)-10,12-dioxo-11-[(phenylmethyl)oxy]-2,3,3a,4,5,5a,6,10,12,13a-decahydro-1H-cyclopenta[e]pyride[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (52 mg, 74%). This material was deproteeted in a second step similar to the procedure described in example Z-37. Thus, from racemic-(3aS,5aS,13aS)-N-[(2,4-difluorophenyl)methyl]-5-(2-methylpropyl)-10,12-dioxo-11-[(phenylmethyl)oxy]-2,3,3a,4,5,5a,6,10,12,13a-decahydro-1H-cyclopenta[e]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (48 mg, 0.081 mmol) and 10% Pd/C (catalytic amount), the title compound was prepared as a white solid after purification by HPLC (30 mg, 75%). ¹H NMR (CDCl₃) 12.59 (s, 1H), 10.42 (s, 1H), 828 (s, 1H), 7.34 (m, 1H), 6.79 (m, 2H), 4.83 (s, 1H), 4.63-4.58 (m, 3H), 4.29 (m, 1H), 4.14 (m, 1H), 2.91 (m, 1H), 2.46-2.32 (m, 3H), 2.15-2.09 (m, 2H), 1.85-1.61 (m, 5H), 1.39 (m, 1H), 0.88 (m, 6H); ES⁺ MS: 501 (M+1).

EXAMPLE Z-50 (3R,11aS)-N-[(2,4)-Difluorophenyl)methyl]-3-ethyl-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (40 mg, 0.09 mmol) and (2R)-2amino-1-butanol (0.02 mL, 0.21 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3R,11aS)-N-[(2,4-difluorophenyl)methyl]-3-ethyl-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (40 mg, 93%). This material was hydrogenated in a second step as described in example Z-2 to give (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-3-ethyl-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (30 mg, 91%) as a white solid. ¹ H NMR (CDCl₃) δ 11.49 (br, 1H), 10.28 (m, 1H), 8.35 (s, 1H), 7.34 (m, 1H), 6.79 (m, 2), 5.30 (m, 1H), 4.62 (m, 2H), 4.45-4.32 (m, 3H), 3.93-3.86 (m, 2H), 2.11 (m, 1H), 1.65 (m, 7H), 0.98 (t, J=7.6 Hz, 3 H); ES⁺ MS; 420 (M+1).

EXAMPLE Z-51 racemic-(4 aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-[2-(4-morpholinyl)ethyl]-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline10-carboxamide

-   a) racemic-1,1-Dimethylethyl [(1S,2R)-2-formylcyclohexyl]carbamate.     As alternative procedure from the one given in example Z-38b     follows: To a solution of Dess-Martin Periodane (564 mg, 1.33 mmol)     in dichloromethane was added racemic-1,1-dimethylethyl     [(1S,2R)-2-(hydroxymethyl)cyclohexyl]carbamate (305 mg, 1.33 mmol,     see example Z-38a) dropwise as a solution in dichloromethane. The     reaction was stirred 1 hour at ambient temperature until judged     complete by TLC (1:1 hexanes: ethyl acetate KMnO₄ stain). The     reaction was quenched with aqueous sodium bicarbonate and sodium     thiosulfate solutions, extracted with dichloromethane, and the     combined organics were dried over sodium sulfate. Silica gel     chromatography (0-50% ethyl acetate/hexanes gradient elution) gave     racemic-1,1-dimethylethyl [(1S,2R)-2-formylcyclohexyl]carbamate     (280, 93%). See example Z-38b for NMR data. -   b)     racemic-{[[(1S,2S)-2-Aminocyclohexyl]methyl}(2-(4-morpholinyl)ethyl]amine     hydrochloride. In a manner similar to that described in example     Z-38c-d from racamic-1,1-dimethylethyl     [(1S,2R)-2-formylcyclohexyl]carbamate (78 mg, 0.344 mmol, prepared     using the procedure from example Z-38b) and     [2-(4-morpholinyl)ethyl]amine (67 mg, 0.515 mmol) was prepared     racemic-{[(1S,2S)-2-aminocyclohexyl]methyl}[2-(4-morpholinyl)ethyl]amine     hydrochloride (95 mg, 78% over 2 steps) as a white solid, ¹H NMR     (methanol-d₄/CDCl₃) 8.18 (br s, 1H), 3.84-3.493 (m, 11H), 3.19-3.119     (m, 5H), 2.42 (m, 1H), 2.11 (br s, 2H), 1.87-1.17 (m, 10H). -   c)

racemic-4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-[2-(4-morpholinyl)ethyl]-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in example Z-35, from racemic-{[(1S,2S)-2-aminocyclohexyl]methyl}[2-(4-morpholinyl)ethyl]amine hydrochloride (95 mg, 0.272 mmol) and 16a (45 mg, 0.0957 mmol) was prepared racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-[2-(4-morpholinyl)ethyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (27 mg, 43%). This material was deprotected in a second step similar to the procedure described in example Z-37. From racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-[2-(4-morpholinyl)ethyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (27 mg, 0.0408 mmol) and 10% Pd/C (1 mg) the title compound was prepared as a white solid after purification by HPLC, ¹H NMR (CDCl₃) 12.30 (br s, <1H), 10.41 (br s, 1H), 8.29 (s, 1H), 7.34 (m, 2H), 6.78 (m, 2H), 4.76 (m, 1H), 4.62-4.54 (m, 3H), 4.29 (m, 2H), 3.65 (m, 4H), 3.01 (m, 1H), 2.76 (m, 2H), 2.58-2.42 (m, 7H), 2.21 (m, 1H), 1.89-1.23 (m, 8H); ES⁺ MS: 572 (M+1).

EXAMPLE Z-52 racemic-(3aR,5aR,13aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,3a,4,5a,6,10,12,13a-decahydrocyclopenta[d]pyrido[1′,2′:4,5]pyrazino[2,1-b][1,3]oxazine 9-carboxamide

-   a) racemic-1,1-Dimethylethyl     [(1S,2R)-2-(hydroxymethyl)cyclopentyl]carbamate,     racemic-{1R,2S)-2-[(1,1-Dimethylethyl)oxy]carbonyl}amino)cyclopentanecarboxylic     acid (22 mg, 0.096 mmol) was dissolved in tetrahydrofuran and placed     in an ice-water bath. Triethylamine was added, followed by the slow     addition of methyl chloroformate. The reaction was stirred ten     minutes in the icebath and sodium borohydride was added. Methanol     was then added slowly and stirring was continued for two hours while     the ice-bath expired. 1 M Potassium hydrogen sulfate was added, the     reaction was partially concentrated, and product was extracted with     dichloromethane. The combined organics were washed with sodium     bicarbonate, brine, and dried over sodium sulfate. Removal of     solvents under reduced pressure afforded racemic-1,1-dimethylethyl     [(1S,2R)-2-(hydroxymethyl)cyclopentyl]carbamate (25 mg, >100%), ¹H     NMR (CDCl₃) 4.50 (br s, 1H), 4.06 (m, 1H), 3.54 (m, 1H), 3.37 (m,     1H), 2.09 (m, 1H), 1.96 (m, 1H), 1.64 (m, 3H), 1.52 (m, 1H), 1.43     (s, 9H), 1.11 (m, 2H). -   b) racemic-[(1R,2S)-2-Aminocyclopentyl]methanol hydrochloride. In a     manner similar to that described in example, from     racemic-1,1-dimethylethyl     [(1S,2R)-2-(hydroxymethyl)cyclopentyl]carbamate and 4 N HCl was     prepared racemic-[(1R,2S)-2-aminocyclopentyl]methanol hydrochloride     (20 mg, quantitative). ¹H NMR (methanol-d4-CDCl₃) 7.76 (br s, <1H),     3.73 (m, 1H), 3.61-3.28 (m, 3H), 2.27 (br s, 1H), 2.01 (m, 2.01 (m,     1H), 1.74-1.70 (m, 2H), 1.56-1.42 (m, 2H), 1.16 (br s, 1H), 1.05 (br     s, 1H). -   c)

racemic-(3aR,13aS)-N-[(2,4-Difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,3a,4,5a,6,10,12,13a-decabydrocyclopenta[d]pyrido[1′,2′:4,5]pyrazino[2,1-b][1,3]oxazine-9carboxamide. In a manner similar to that described in example Z-35, from racemic-[(1R,2S)-2-aminocyclopentyl]methanol hydrochloride (20 mg, 0.132 mmol) and 16a (24 mg, 0.051 mmol) was prepared racemic-(3aR,13aS)-N-[(2,4-difluorophenyl)methyl]-10,12-dioxo-11-[(phenylmethyl)oxy]-1,2,3,3a,4,5a,6,10,12,13a-decahydrocyclopenta[d]pyrido[1′,2′:4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide (7 mg, 26%) as a white solid. This material was deprotected in a second step similar to the procedure described in example 2-37. Thus, from racemic-(3aR,13aS)-N-[(2,4-difluorophenyl)methyl]-10,12-dioxo-11-[(phenylmethyl)oxy]-1,2,3,3a,4,5a,6,10,12,13a-decahydrocyclopenta[d]pyrido[′,2′:4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide (7 mg, 0.012 mmol) and 10% Pd/C (1 mg), racemic-(3a R,13aS)-N-[(2,4-difluorophenyl)methyl]-11-hydroxy-10,12-dioxo-1,2,3,3a,4,5a,6,10,12,13a-docahydrocyclopenta[d]pyrido[1′,2′:4,5]pyrazino[2,1-][1,3]oxazine-9-carboxamide (4 mg, 72%) white solid. ¹H NMR (CDCl₃) 12.20 (br s, 1H), 10.37 (br s, 1H), 8.31 (s, 1H), 7.35 (m, 1H), 6.80 (m, 2H), 5.16 (m, 1H), 4.77 (m, 1H), 4.64 (m, 2H), 4.28 (m, 1H), 4.09 (m, 1H), 3.97 (m, 1H), 3.45 (m, 1H), 2.49-2.20 (m, 2H), 1.89-1.58 (m, 4H), 0.936-0.840 (m, 1H); ES⁺ MS: 446 (M+1).

EXAMPLE Z-53 racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-methyl-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide

-   a) racemic-{[(1S,2S)-2-Aminocyclohexyl]methyl}methylamine     hydrochloride. In a manner similar to that described in example     Z-38c-d from racomic-1,1-dimethylethyl     [(1S,2R)-2-formylcyclohexyl]carbamate (0.430 mmol) and methyl amine     (0.5 mL of a 2 M tetrahydrofuran solution) was prepared     racemic-{[(1S,2S)-2-aminocyclohexyl]methyl}methylarnine     hydrochloride in two steps as a white solid (46 mg, 63% 2 steps). ¹H     NMR (methanol-d₄/CDCl₃) 9.05 (br s, <1H), 8.72 (br s, <1H), 8.24 (br     s, 1H), 3.34 (m, 1H), 3.29 (m, 1H), 2.85 (br ,s, 1H), 2.66 (br s,     4H), 2.38 (br s, 1H), 2.07-1.83 (m, 2H), 1.67-1,14 (m, 6H). -   b)

racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-methyl-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in example Z-35, from racemic-{[(1S,2S)-2-aminocyclohexyl]methyl}methylamine hydrochloride (46 mg, 0.215 mmol) and 16a (35 mg, 0.0744 mmol) was prepared racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-methyl-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′, 2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide(17 mg, 41%) as a white solid. This material was deprotected in s second step similar to the procedure described in example Z-37. Thus, from racemic (4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-methyl-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (17 mg, 0.0302 mmol) and 10% Pd/C (1 mg) was prepared the title compound as a white solid (9 mg, 64%), ¹H NMR (CDCl₃) 10.44 (m, 1H), 8.29 (s, H), 7.34 (m, 1H), 6.79 (m, 2H), 4.78 (m, 1H), 4.62 (br s, 2H), 4.29 (br s, 2H), 3.41 (s, 1H), 2.92 (m, 1H), 2.66 (m, 1H), 2.35-2.25 (m, 4H), 1.90-1.74 2H), 1.67-1.24 (m, 6H); ES⁺ MS; 473(M+1).

EXAMPLE Z-54 racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-[2-(methyloxy)ethyl]-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide

-   a)     racemic-{[(1S,2S)-2-Aminocyclohexyl]methyl}[2-(methyloxy)ethyl]amine     hydrochloride.

In a manner similar to that described in example Z-38c-d from racemic-1,1-dimethylethyl [(1S,2R)-2-formylcyclohexyl]carbamate (93 mg, 0.410 mmol) and [2-(methyloxy)ethyl]amine (0.05 mL, 0.615 mmol) was prepared in two steps racemic-{[(1S,2S)-2-aminocyclohexyl]methyl}[2-(methyloxy)ethyl]amine hydrochloride (63 mg, 60% 2 steps) as a white solid. ¹H NMR (methanol-d₄/CDCl₃) 9.02 (br s, <1H), 8.78 (br s, <1H), 8.29 (br s, 1H), 3.69 (br s, 2H), 3.46)s, 3H), 3.36-3.18 (m, 4H), 2.97 (br s, 1H), 2.46 (br s, 1H), 1.86-1.40 (m, 8H).

-   b)

racemic-4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-[2-(methyloxy)ethyl]-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1,′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in example Z-35, from racamic-{[(1S,2S)-2-aminocyclohexyl]methyl}[2-(methyloxy)ethyl]amine hydrochloride (63 mg, 0.244 mmol) and 16a (40 mg, 0.0851 mmol) was prepared racomic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-[2-(methyloxy)ethyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,18,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (44 mg, 81%) as a white solid. This material was deprotected in a second step similar to the procedure described in example Z-37. Thus, from racemic-(4aS,6aS,14aS)-[(2,4-difluorophenyl)methyl]-6-[2-(methyloxy)ethyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodocahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (44 mg, 0.0726 mmol) and 10% Pd/C (1 mg) the title compound was prepared as a white solid (37 mg, quantitative). ¹H NMR (CDCl₃) 12.60 (br s, 1H), 10.47 (m, 1H), 8.28 (a, 1H), 7.34 (m, 1H), 6.79 (m, 2H), 4.81 (m, 1H), 4.64 (m 8H), 4.51 (m, 1H), 4.26 (m, 1H), 3.63 (m, 1H), 3.31 (s, 3H), 3.19 (m, 1H), 2.86 (m, 1H), 2.67 (2m, 2H), 2.21 (m, 1H), 1.91-1.78 (m, 2H), 1.671.52 (m, 4H), 1.46-1.24 (m, 3H); ES⁺ MS: 517 (M+1).

EXAMPLE Z-55 racemic-(4aS,6aS,14aS)-6-[2-(Acetylamino)ethyl]-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-cayboxamide

-   a)     raceimic-N-[2-({[(1S,2S)-2-Aminocyclohexyl]methyl}amino)ethyl]acetamide     hydrochloride. In a manner similar to that described in example     Z-38c-d from racemic-1,1,-dimethylethyl     [(1S,2R)-2-formylcyclohexyl]carbamate (93 mg, 0.41 mmol) and     N-(2-aminoethyl)acetamide (63 mg, 0.615 mmol),     racemic-N-[2-({[(1S,2S)-2-aminocyclohexyl]methyl}amino)ethyl]acetamide     hydrochloride was prepared in two steps as a white solid (82 mg),     71% 2 steps), ¹H NMR (methanol-d₄/CDCl₃) 8.86 (br s, 1H), 8.20 (br     s, 1H), 3.62-3.51 (m, 3H), 3.40-3.28 (m, 4H), 3.22-2.93 (m, 3H),     2.47 (m, 1H), 2.08-2.06 (m, 4H), 1.83-1.75 (m, 2H), 1.56-1.44 (m,     3H), 1.23 (m, 1H). -   b)

racemic-4aS,6aS,14aS)-6-[2-(Acetylamino)ethyl]-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in example Z-35, from racemic-N-[2-({[(1S,2S)-2-aminocyclohexyl]methyl}amino)ethyl]acetamide hydrochloride (82 mg, 0.349 mmol) and 16a (50 mg, 0.106 mmol) was prepared the title compound (24 mg, 36%). This material was deprotected in a second step similar to the procedure described in example Z-37. Thus, from racemic (4aS,6aS,14aS)-6-[2-(acetylamino)ethyl]-N-[(2,4-difluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (24 mg, 0.0379 mmol) and 10% Pd/C (1 mg) was prepared the title compound as a white solid after purification by HPLC. ¹H NMR (CDCl₃) 12.59 (s, 1H), 10.44 (s, 1H), 8.35 (s, 1H), 7.32 (m, 1H), 6.79 (m, 2H), 5.86 (s, 1H), 4.78 (m, 1H), 4.61-4.50 (m, 3H), 4.30 (m, 1H), 3.35 (m, 1H), 3.18 (m, 1H), 2.96 (m, 1H), 2.76 (m, 2H), 2.48 (m, 1H), 2.19 (m, 1H), 1.89-1.23 (m, 12H); ES⁺ MS: 544 (M+1).

EXAMPLE Z-56 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-3ethyl-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-16a (4.0 mg, 0.09 mmol) and (2S)-2-amino-1-butanol (0.1 mL) were reacted in dichlorornethane (2 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-ethyl-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (39 mg, 90%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-ethyl-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazoio[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (37 mg, 99%) as a tinted white solid. ¹H NMR (CDCl₃) δ 11.47 (br, 1H), 10.26 (m, 1H), 8.35 (s, 1H), 7.32 (m, 1H), 6.77 (m, 2), 5.29 (m, 1H), 4.60 (m, 2H), 4.47-4.32 (m, 3H), 3.93-3.85 (m, 2H), 2.08 (m, 1H), 1.68 (m, 1H), 0.95 (t, J=7.6 Hz, 3H); ES⁺ MS: 420 (M+1).

EXAMPLE Z-57 (3S,11aR)-3-Butyl-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2, 16a (40 mg, 0.09 mmol) and (2S)-2-amine-1-hexanol (100 mg) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-3-butyl-N-[(2,4-difluorophenyl)methyl]-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (43 mg, 94%). This material was hydrogenated in a second step as described in example Z-2 to give (3S,11aR)-3-butyl-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (33 mg, 92%) as a tinted white solid. ¹H NMR (CDCl₃) δ 11.48 (br, 1H), 10.27 (br, 1H), 8.36 (br, 1H), 7.31 (m, 1H), 6.77 (m, 2), 5.28 (m, 1H), 4.59-4.36 (m, 5H), 3.83 (m, 2H), 2.08 (m, 1H), 1.58 (m, 1H), 1.39-1.23 (m, 4H), 0.90 (t, J=6.8 Hz, 3H); ES⁺MS: 448 (M+1).

EXAMPLE Z58 (3S,11aR)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-[(4-hydroxyphenyl)methyl]-5,7-dioxo-2,3,5,7,11,11a -hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (4.0 mg, 0.09 mmol) and 4-[(2S)-2-amino-3-hydroxypropyl]phenol (43 mg, 0.21 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-3-[(4-hydroxyphenyl)methyl]-5,7-dioxo-6-[(phenylmethyl)oxy]-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (10 mg, 20%). This material was hydrogenated in a second step as described in example Z-2 and purified via preparative HPLC to give (3S,11aR)-N-[(2,4-difluorophenyl)methyl]-6-hydroxy-3-[(4-hydroxyphenyl)methyl]-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-a]pyrido[1,2-d]pyrazine-8-carboxamide (7 mg, 63%) as a white solid. ¹H NMR (CD₃OD) δ 10.43 (m, 1H), 8.34 (s, 1H), 7.33 (m, 1H), 7.00 (d, J=8.4 Hz, 2H), 6.82 (m, 2H), 6.71 (d, J=8.4 Hz, 2H), 5.05 (m, 1H), 4.67-4.57 (m, 4H), 4.21 (dd, J=8.8, 7.2 Hz, 1H), 3.94 (dd, J=8.8, 6.4 Hz, 1H), 3.21 (dd, J=13.2, 3.2 Hz, 1H), 2.90 (dd, J=13.6, 8.8 Hz, 1H); ES⁺ MS: 498 (M+1).

EXAMPLE Z-59 (4S,12aS)-1-Cyclobutyl-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyride[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl]cyclobutylamino dihydrochloride was prepared     in a similar manner as described in oxample Z-47. ¹H NMR (400 MHz,     CDCl₃/CD₃OD) δ 1.23 (d, J=6.4 Hz, 8H), 1.69-2.26 (m, 8H), 2.83 (m,     2H), 3.31-3.33 (m, 1H), 3.55 (m, 1H), 8.08 (br, <1H), 9.07 (br,     <1H). -   b)

(4S,12aS)-1-Cyclobutyl-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (80 mg, 0.17 mmol) and free based [(3S)-3-aminobutyl]cyclobutylamine (96 mg, 0.68 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-1-cyclobutyl-N-[(2,4 -difluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (68 mg, 70%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-1-cyclobutyl-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′, 2′:4,5]pyrazino[1,2-a]pyrimidine-9carboxamide (57 mg, 100%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.31 (d, J=6.8 Hz, 3H), 1.46-1.70 (m, 4H), 1.91-2.12 (m, 4H), 2.52 (m, 1H), 2.90-2.93 (m, 1H), 3.06 (m, 1H), 4.16-4.29 (m, 3H), 4.57-4.66 (m, 2H), 4.09-5.05 (m, 1H), 6.75-6.82 (m, 2H), 7.32-7.38 (m, 1H), 8.20 (s, 1H), 10.44 (s, 1H), 12.51 (s, 1H); ES⁺ MS: 473 (M+1).

EXAMPLE Z-60 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl]tetrahydro-2H-thiopyran-4-ylamine     dihydrochloride was prepared in a similar manner as described in     example Z-47. ¹H NMR (400 MHz, CDCl₃/CD₃OD) δ 1.21 (d, J=6.4 Hz ,     3H), 1.65-1.75 (m, 2H), 1.90-2.10 (m, 2H), 2.35 (m, 2H), 2.56-2.61     (m, 4H), 2.92-2.98 (m, 3H), 3.27-3.31 (m, 1H), 8.05 (br, <1H), 8.90     (br, <1H). -   b)

(4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-oetahydropyrido[1,′2′:4,5]pyrazino[(1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z2, 16a (80 mg, 0.17 mmol) and free based [(3S)-3-aminobutyl]tetrahydro-2H-thiopyran-4-ylamine (108 mg, 0.58 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (56 mg, 54%) as a film. This material was debenzylated in a second step to in a manner similar to Z-26 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]py razino[1,2-a]pyrimidine-9-carboxamide (56 mg, >1.00%) as an off-white solid. ¹H NMR (400 MHz, CDCl3) δ 1.30 (d, J=6.8 Hz , 3H), 1.54-1.58 (m, 1H), 1.72-1.82 (m, 3H), 1.97-2.11 (m, 2H), 2.60-2.76 (5H), 2.86 (m, 2H), 4.17-4.30 (m, 2H), 4.62-4.66 (m, 3H), 4.92-4.96 (m, 1H), 6.75-6.82 (m, 2H), 7.32-7.38 (m, 1H), 8.31 (s, 1H), 10.46 (s, 1H), 12.48 (s, 1H); ES⁺ MS: 519 (M+1).

EXAMPLE Z-61 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Amino-5-methylhexyl](2-methylpropyl)amine dihydrochloride     was prepared in a similar manner as described in example Z-32. ¹H     NMR (400 MHz, CDCl₃/CD₃OD) δ 0.87 (d, J=6.4 Hz ,6H), 0.97 (d, J=6.8     Hz , 6H), 1.34-1.41 (m, 1H), 1.45-1.52 (m, 1H), 1.58-1.66 (m, 1H),     2.01-2.13 (m, 2H), 2.72-2.73 (m, 2H), 3.03-3.06 (m, 2H), 3.29 (m,     2H), 8.07 (br, <1H), 8.71 (br, <1H). -   b)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16a (80 mg, 0.17 mmol) and free based [(3S)-3-amino-5-methylhexyl](2-methylpropyl)arnine (117 mg, 0.63 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-1,4-bis(2-methylpropyl)-6,8-dioxo-7-[(phenyl methyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (68 mg, 66%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (66 mg, 97%) as an off-white solid, ¹H NMR (400 MHz, CDCl3) δ 0.74 (d, J=6.4 Hz, 3H), 0.84 (d, J=6.4 Hz, 3H), 0.97-1.00 (m, 6H), 1.37-1.83 (m, 2.03-2.12 (m, 2H), 2.21-2.28 (m, 1H), 2.77 (m, 1H), 2.90-2.93 (m, 1H), 4.19-4.40 (m, 3H), 4.59-4.70 (m, 2H), 4.96-4.97 (m, 1H), 6.77-6.83 (m, 2H), 7.33-7.39 (m, 1H), 8.28 (s, 1H), 10.47 (s, 1H), 12.59 (br, 1H); ES⁺ MS: 517 (M+1).

EXAMPLE Z-62 racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-(2-hydroxyethyl)-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide

-   a) racemic-2-({[(1S,2S)-2-Aminocyclohexyl]methyl}amino)ethanol     hydrochloride. In a manner similar to that described in example     Z-55a, from racemic-1,1-dimethylethyl     [(1S,2R)-2-formylcyclohexyl]carbamate (1.12 mg, 0.497 mmol) and     2-aminoethanol (0.04 mLm 0.746 mmol) was prepared     racemic-2-({[(1S,2S)-2-aminocyclohexyl]methyl}amino)ethanol     bis-hydrochloride in two steps (102 mg, 84% over 2 steps). ¹H NMR     (methanol-d₄/CDCl₃) 8.81-8.40 (m, <2H), 8.16 (br s, 1H), 4.02-3.93     (m, 2H), 3.80 (br s, 2H), 3.53 (m, 1H), 3.36-2.93 (m, 6H), 2.41 (br     s, 1H), 2.05 (m, 1H), 1.75-1.41 (m, 4H). -   b)

racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-6-(2-hydroxyethyl)-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in example Z-35, from 16a (45 mg, 0.0957 mmol) and racemic-2-({[(1S,2S)-2-aminocyclohexyl]methyl}amino)ethanol hydrochloride (102 mg, 0.418 mmol) was prepared racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-(2-hydroxyethyl)-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (7 mg, 12%) as a white solid after silica gel chromatography (1.12% methanol/dichloromethane gradient elution). This material was deprotected in a second step similar to the procedure described in example Z-37, Thus, from racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-6-(2-hydroxyethyl)-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (7 mg, 0.0118 mmol) the title compound was prepared after purification by HPLC (3 mg, 50%). ¹H NMR (CDCl₃) 12.57 (br s, 1H), 10.45 (m, 1H), 8.20 (s, 1H), 7.34 (m, 1H), 6.78 (m, 2H), 4.80 (m, 1H), 4.71 (a, 1H), 4.62 (m, 2H), 4.44 (m, 1H), 4.33 (m, 1H), 3.75 (m, 1H), 3.62-3.20 (m, 3H), 3.13 (m, 1H), 2.74-2.71 (m, 2H), 2.24 (m, 1H), 1.90-137 (m, 12H), 1.27-1.23 (m, 3H)1.12 (m, 1H); ES⁺ MS: 503 (M+1).

EXAMPLE Z-63 racemic-(4aS,6aS,14aS)-6-Cyclopropyl-N-[(2,4-difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2a]quinazoline-10-carboxamide

-   a) racemic-(1S,2S)-2-[(Cyclopropylamino)methyl]cyclohexanamine     hydrochloride. In a manner similar to that described in example     Z-55a, from racemic-1,1-dimethylethyl     [(1S,2R)-2-formylcyclohexyl]carbamate (112 mg, 0.497 mmol) and     cyclopropylamine (0.05 mL, 0.746 mmol) was prepared     racemic-(1S,2S)-2-[(cyclopropylamino)methyl]cyclohexanamine bis     hydrochloride salt in two steps (102 mg, 86% over 2 steps). This     material was used without further purification, ¹H NMR     (methanol-d₄/CDCl₃) 8.31 (br s, 1H), 3.75 (br s, 1H), 3.54 (m, 1H),     2.96 (m, 1H), 2.71 (m, 1H), 2.27 (m, 1H), 1.94 (m, 1H), 1.76-1.15     (m, 8H), 0.88-0.78 (m, 3 H). -   b)

racemic-(4aS,6aS,14aS)-6-Cyclopropyl-N--[(2,4-difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide. In a manner similar to that described in example Z-35, from 16a (45 mg, 0.0957 mmol) and racemic-(1S,2S)-2-[(cyolopropylamino)methyl]cyclohexanamine hydrochloride (102 mg, 0.425 mmol) was prepared racemic-(4aS,6aS,14aS)-6-cyclopropyl-N-[(2,4-difluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodocahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide as a white solid after silica gel chromatography (1-12% methanol/dichloromethane gradient elution). This material was deprotected in a second step similar to the procedure described in example Z-37. Thus, from racemic-(4aS,6aS,14aS)-6-cyclopropyl-N-[(2,4-difluorophoeyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (56 mg, 0.0949 mmol) the title compound was prepared as a white solid (41 mg, 81%). ¹H NMR. (CDCl₃) 12.10 (br s, <1H), 10.45 (m, 1H), 8.27 (s, 1H), 7.33 (m, 1H), 6.88 (m, 2H), 4.77 (m, 1H), 4.61-4.49 (m, 4H), 4.33 (m, 1H), 2.94 (m, 1H), 2.79 (m, 1H), 2.17 (m, 1H), 1.86-0.86 (m, 10H), 0.658 (m, 1H), 0.499-0.32 (m, 2H); ES⁺ MS: 499 (M+1),

EXAMPLE Z-64 racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-6-[2-(1-pyrrolidinyl)ethyl]-1,2,3,4,4a,5,6,6a7,11,13,14a-dodecahydropyrido[1′,2′:4,5]-pyrazino[1,2-a]quinazoline-10-carboxamide formic acid salt

-   a)     racemic-(1S,2S)-2-({[2-(1-Pyrrolidinyl)ethyl]amino}methyl)cyclohexanamine     hydrochloride. In a manner similar to that described in example     Z-55a, from racemic-1,1-dimethylethyl     [(1S,2R)-2-formylcyclohoxyl]carbamate (1.12 mg, 0.4.97 mmol) and     2-(1-pyrrolidinyl)ethanamine (0.09 mL, 0.746 mmol) was prepared     racemic-(1S,2S)-2-({[2-(1-pyrrolidinl)ethyl]amino}methyl)cyclohexanamine     (88 mg, 60% 2 steps) as the bis hydrochloride salt in two steps as a     white solid. ¹H NMR (methanol-d₄/CDCl₃) 9.68 (br s, <1H), 9.24 (br     s, <1H), 8.25 (br s, 1H), 3.75-3.04 (m, 11H), 2.87 (br s, 1H),     2.06-1.20 (m, 12H). -   b)

racemic-(4aS,6aS,14aS)-N-[(2,4-Difluorophenyl)methyl]-12-hydroxy-11,13-dioxo-6-[2-(1-pyrrolidinyl)ethyl]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyridor[1′,2′:4,5]pyrazino[1,2a]quinazoline-10-carboxamide formic acid salt.

In a manner similar to that described in example Z-35, from 16a (30 mg, 0.0638 mmol) and racemic-(1S,2S)-2-({[2-(1-pyrrolidinyl)ethyl]amino}methyl)cyclohexanamine hydrochloride (88 mg, 0.296 mmol) was prepared racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-6-[2-(1-pyrrolidinyl)ethyl]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide as a white solid (31 mg, 76%) after silica gel chromatography (1-12% methanol/dichloromethane gradient elution). This material was deprotected in a second step similar to the procedure described in example Z37. Thus, from racemic-(4aS,6aS,14aS)-N-[(2,4-difluorophenyl)methyl]-11,13-dioxo-12-[(phenylmethyl)oxy]-6-[2-(1-pyrrolidinyl)ethyl]-1,2,3,4,4a,5,6,6a,7,11,13,14a-dodecahydropyrido[1′,2′:4,5]pyrazino[1,2-a]quinazoline-10-carboxamide (31 mg, 0.048 mmol) the title compound was prepared as a yellow solid after purification by HPLC (18 mg, 66%). ¹H NMR (CDCl₃) 10.39 (br s, 1H), 8.56 (br s, 1H), 8.39 (br s, 1H), 7.34 (m, 1H), 6.78 (m, 2H), 4.76-4.40 (m, 6H), 3.26-2.89 (m, 7H), 2.73 (m, 1H), 2.15 (m, 1H), 2.02-1.18 (m, 14H); ES⁺ MS: 556 (M+1).

EXAMPLE Z-65 (4a,14aS)-N-[(2,4-Difluorophenyl)methyl]-9-hydroxy-8,10-dioxo-2,3,4,4a,5,6,8 10,14,14a-decahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide

-   a) {2-[(2S)-2-Piperidinyl]ethyl}amine. This compound was prepared in     a similar manner as its enantiomer described in example Z-42a. -   b)

(4aS,14aS)-N[(2,4-Difluorophenyl)methyl]-9-hydroxy-8,10-dioxo-2,3,4,4a,5,6,8,10,14,14a-docahydro-1H-pyrido[1,2-c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11carboxamide. In a manner similar to that described in example Z-35, from {2-[(2S)-2-piperidinyl]ethyl}amine (28 mg, 0.218 mmol) and 16a (30 mg, 0.0638 mmol) was prepared (4aS,14aS)-N-[(2,4-difluorophenyl)methyl]-8,10-dioxo-9-[(phenylmethyl)oxy]-2,3,4,4a,5,6,8,10,14,14a-docahydro-1H-pyrido[1,2c]pyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-11-carboxamide (29 mg, 82%). This material was deproteeted in a second step similar to that described in example Z-37 to give the title compound as a white solid (26 mg, quantitative). ¹H NMR (CDCl₃) δ 12.44 (br s, 1H), 10.48 (s, 1H), 8.26 (s, H), 7.35 (m, 1H), 6.80 (m, 2H), 4.68-4.57 (m, 2H), 4.38 (m, 1H), 4.20 (m, 1H), 3.93 (s, 1H), 3.63-3.39 (m, 2H), 2.91 (m, 2H), 2.29 (br s, 1H), 2.02 (m, 1H), 1.69-1.45 (m, 4H), 1.30-1.24 (m, 2H), 1.12 (br s, 1H); ES⁺ MS; 459 (M+1).

EXAMPLE Z-66 (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl][2-(methyloxy)ethyl]amine bis hydrochloride.     In a manner similar to that described in example Z-47, from     1,1-dimethylethyl [(1S)-1-methyl-3-oxopropyl]carbamate (76 mg, 0.406     mmol) and 2-(methyloxy)ethyl]amine (0.05 mL, 0.609 mmol) was     prepared [(3S)-3-aminobutyl][2-(methyloxy)ethyl]amine as the bis     hydrochloride salt in two steps (10 mg, quantitative). ¹H NMR     (methanol-d₄/CDCl₃) δ 9.02 (<1H), 8.24 (<1H), 3.68 (br s, 2H), 3.49     (br s, 1H), 3.34 (br s, 4H), 3.15 (br s, 4H), 2.26-2.11 (m, 2H),     1.35 (br s, 3H). -   b)

(4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. In a manner similar to that described in example Z-35, from 16 (15 mg, 0.034 mmol) and [(3S)-3-Aminobutyl][2-(methyloxy)ethyl]amine bis hydrochloride (19 mg, 0.087 mmol), (4S,12aS)-N-[(4-fluorophenyl)methyl]-4-methyl-1-[2-(methyloxy)ethyl]-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2a]pyrimidine-9-carboxamide was prepared as a white solid after silica gel chromatography (1-12% methanol/dichloromethane). This material was deprotected in a second step similar to that described in example Z-37 to give the title compound as a yellow solid (9 mg, 60%, 2 steps). ¹H NMR (CDCl₃) δ 12.56 (s, 1H), 10.51 (m, 1H), 8.29 (s, 1H), 7.32 (m, 2H), 6.98 (m, 2H), 5.03 (m, 1H), 4.65-4.59 (m, 2H), 4.53 (m, 1H), 4.21 (m, 1H), 3.61-3.40 (m, 2H), 3.34-3.13 (m, 3H), 3.08 (m, 1H), 2.94-2.84 (m, 2H), 2.68 (m, 1H), 2.07 (m, 1H), 1.50 (m, 1H), 1.35 (d, J=7.2 Hz., 3H), 1.14 (m, 1H); ES⁺ MS: 459 (M+1).

EXAMPLE Z-67 (4S,12aS)-1-Cyclobutyl-N[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl]cyclobutylamine bis-hydrochloride. In a manner     similar to that described in example Z-47, from 1,1-dimethylethyl     [(1S)-1-methyl-3-oxopropyl]carbamate (76 mg, 0.406 mmol) and     cyclobutylamine (0.05 mL, 0.609 mmol) was prepared     [(3S)-3-Aminobutyl]cyclobutylamine bis-hydrochloride in two steps     (23 mg, 27%). ¹H NMR (methanol-d₄/CDCl₃) δ 8.86 (s, <1H), 7.97 (s,     <1H), 3.46 (m, 1H), 3.21 (m, 1H), 2.74 (m, 2H), 2.14-2.08 (m, 4H),     1.94-1.62 (m, 5H), 1.13 (d, J=6 Hz, 1H). -   b)

(4S,12aS)-1-Cyclobutyl-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. In a similar manner to that described in example Z-35a, from 16 (18 mg, 0.39 mmol) and [(3S)-3-Aminobutyl]cyclobutylamine bis-hydrochloride (23 mg, 0.107 mmol),

(4S,12aS)-1-cyclobutyl-N-[(4-fluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide was prepared as a white solid. This material was deprotected in a second step similar to that. described in example Z-37 to give the title compound as a white solid after purification by HPLC (4.5 mg, 25% 2 steps), ¹H NMR (CDCl₃) δ 12.54 (s, 1H), 10.48 (s, 1H), 8.20 (s, 1H), 7.31 (m, 2H), 6.98 (m, 2H), 5.02 (m, 1H), 4.61-4.57 (m, 2H), 4.26-4.14 (m, 3H), 3.05 (m, 1H), 2.90 (m, 1H), 2.49 (m, 1H), 2.12 (m, 1H), 2.05-1.87 (m, 3H), 1.84-1.61 (m, 3H), 1.46 (m, 1H), 1.32 (m, 3H); ES⁺ MS: 455 (M+1).

EXAMPLE Z-68 (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylproyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl](2-methylpropyl)amine bis hydrochloride. In a     manner similar to that described in example Z-47, this compound was     prepared from 1,1-dimethylethyl [(1S)-1-methyl-3-oxopropyl]carbamate     (76 mg, 0.406 mmol) and (2-methylpropyl)amine (0.06 mL, 0.609 mmol)     in two steps as the bis-hydrochloride salt (22 mg, 25%). ¹H NMR     (methanol-d₄/CDCl₃) δ 3.23 (br s, 1H), 2.91 (br s, 2H), 2.64 (m,     2H), 2.02-1.93 (m, 3H), 1.17 (m, 3H), 0.88 (m, 6H). -   b)

(4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. In a similar manner to that described in example Z-35, from 16 (16 mg, 0.035 mmol) and [(3S)-8-Aminobutyl](2-methylpropyl)amine bis-hydrochloride (20 mg, 0.0925 mmol), (4S,12aS)-N-[(4-fluorophenyl)methyl]-4-methyl-1-(2-methylpropyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide was prepared as a white solid. This material was deprotected in a second step similar to that described in example Z-37 to give the title compound as a tan solid (13 mg, 68% 2 steps). ¹H NMR (CDCl₃) δ 12.57 (s, 1H), 10.46 (s, 1H), 8.27 (s, 1H), 7.32 (m, 2H), 6.99 (m, 2H), 4.98 (m, 1H), 4.63-4.54 (m 2H), 4.45 (m, 1H),4.26-4.16 (m, 2H), 2.91 (m, 1H), 2.77 (m, 1H), 2.24 (m, 1H), 2.14-2.03 (m, 2H), 1.63 (m, 1H), 1.48 (m, 1H), 1.33 (m, 3H), 1.09 (m, 1H), 0.850 (ni, 3H), 0.789 (m, 3H); ES⁺ MS: 457 (M+1).

EXAMPLE Z-69 (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl]methylamine bis-hydrochloride. In a manner     similar to that described in example Z-47, this compound was     prepared from 1,1-dimethylethyl [(1S)-1-methyl-3-oxopropyl]carbamate     (76 mg, 0.409 mmol) and excess methylamine (2 M in tetrhydrofuran)     in two steps as the his hydrochloride salt (17% 2 steps). ¹H NMR     (methanol-d₄/CDCl₃) δ 3.16 (m, 1H), 3.08 (s, 2H), 2.83 (m, 2H), 2.45     (s, 3H), 1.88 (m, 1H), 1.75 (m, 1H), 1.09 (m, 3H). -   b)

(4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. In a similar manner to that described in example Z-35, from 16 (18 mg, 0.0398 mmol) and [(3S)-3aminobutyl]methylamine bis-hydrochloride (19 mg, 0.109 mmol, (4S,12aS)-N-[(4-fluorophenyl)methyl]-1,4-dimethyl-6,8-dioxol7-[(phenylmethyl)oxy]-1,2,8,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide was prepared as a white solid. This material was deprotected in a second step similar to that described in example Z-37 to give the title compound as a tan solid (7 mg, 4.4% 2 steps), ¹H NMR (CDCl₃) δ 12.53 (s, 1H), 10.47 (s, 1H), 8.29 (s, 1H), 7.32 (m, 2H), 6.99 (m, 2H), 5.04 (1H), 4.60 (m, 2H), 4.23 (s, 3H), 2.83-2.80 (m, 2H), 2.82 (s, 3H), 2.13 (m, 1H), 1.48 (m, 1H), 1.34 (m, 3H); ES⁺ MS: 4.15 (M+1).

EXAMPLE Z-70 (4S,12aS)-N-[(4-Fluorophonyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2, 16 (25 mg, 0.055 mmol) and free based [(3S)-3-aminobutyl]tetrahydro-2H-thiopyran-4-ylamine (48 mg, 0.26 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(4′-fluorophenyl)methyl]-4-methyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,1,2a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (16 mg, 49%) as a film. This material was debenzylated in a second step in a manner similar to Z-26 to give (4S,12aS)-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-6,8-dioxo-1-(tetrahydro-2H-thiopyran-4-yl)-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (8 mg, 59%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.30 (d, J=7.2 Hz, 3H), 1.53-1.58 (m, 1H), 1.72-2.10 (m, 5H), 2.56-2.76 (m, 5H), 2.84-2.87 (m, 2H), 4.18 (dd, J=2,8, 14.0 Hz, 1H), 4.26 (dd, J=3.4, 14.2 Hz, 1H), 4.92-4.97 (m, 1H), 6.96-7.00 (m, 2H), 7.29-7.36 (m, 2H), 8.31 (s, 1H), 10.48 (m, 1H), 12.48 (br, 1H); ES⁺ MS: 501 (M+1).

EXAMPLE Z-71 (4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,812,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

-   a) [(3S)-3-Aminobutyl]methylamine dihydrochloride was prepared in a     similar manner as described in example Z-47. ¹H NMR (400 MHz, CDCl₃)     δ0 1.18 (d, J=6.8 Hz, 3H), 1.82-1.91 (m, 1H), 1.94-2.03 (m, 1H),     2.53 (s, 3H), 2.89-2.93 (m, 2H), 3.22-3.30 (m, 1H), 8.02 (br, <1H),     8.81 (br, <1H). -   b)

(4S,12aS)-N-[(2,4-Difluorophenyl)methyl]-7-hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide. The title compound was made in two steps using a similar process to that described in example Z-2, 16a (40 mg, 0.085 mmol) and free based [(3S)-3-aminobutyl]methylamine (24 mg, 0.23 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-1,4-dimethyl-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-oetahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (39 mg, 89%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(2,4-difluorophenyl)methyl]-7-hydroxy-1,4-dimethyl-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (32 mg, 97%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.33 (d, J=6.4 Hz, 3H), 1.46-1.50 (m, 1H), 2.12-2.14 (m, 1H), 2.32 (s, 3H), 2.83 (m, 2H), 4.24 (nn, 3H), 4.62 (m, 2H), 5.02 (m, 1H), 6.77-6.79 (m, 2H), 7.33 (m, 1H), 8.30 (s, 1H), 10.43 (s, 11-I), 12.50 (br, 1H); ES⁺ MS: 433 (M+1).

EXAMPLE Z-72 (4S12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2, 16 (27 mg, 0.060 mmol) and free based [(3S)-3-aminobutyl](1-methylethyl)amine (67 mg, 0.51 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(4-fluorophonyl)methyl]-4-methyl-1-(1-methylethyl)-6,8-dioxo-7-[(phenyl methyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (18 mg, 56%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(4-fluorophenyl)methyl]-7-hydroxy-4-methyl-1-(1-methylethyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (15 mg, >100%) as an off-white solid. ¹H NMR (400 MHz, CDCl₃) δ 1.02 (d, J=6.4 Hz, 3H), 1.07 (d, J=6.4 Hz, 3H), 1.32 (d, J=6.8 Hz, 3H), 1.54-1.58 (m, 1H), 1.94-2.03 (m, 1H), 2.71-2.76 (m, 1H), 2.82-2.88 (m, 1H), 3.13-3.16 (m, 1H), 4.16-4.19 (m, 1H), 4.30-4.33 (m, 1H), 4.48 (m, 1H), 4.55-4.65 (m, 2H), 4.97-5.00 (m, 1H), 6.97-7.01 (m, 2H), 7.30-7.34 (m, 2H), 8.28 (s, 1H), 10.51 (m, 1H), 12.55 (s, 1H); ES⁺ MS: 443 (M+1).

EXAMPLE Z-73 (4S,12aS)-N-[(4-Fluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a -octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide

The title compound was made in two steps using a similar process to that described in example Z-2. 16 (25 mg, 0.055 mmol) and free based [(3S)-3-amino-5-methylhexyl](2-methylpropyl)amine (21 mg, 0.11 mmol) were reacted in dichloromethane (2 mL) with acetic acid to give (4S,12aS)-N-[(4-fluorophenyl)methyl)]-4-bis(2-methylpropyl)-6,8-dioxo-7-[(phenylmethyl)oxy]-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′:4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (8 mg, 25%) as a film. This material was hydrogenated in a second step as described in example Z-2 to give (4S,12aS)-N-[(4-fluorophenyl)methyl]-7-hydroxy-1,4-bis(2-methylpropyl)-6,8-dioxo-1,2,3,4,6,8,12,12a-octahydropyrido[1′,2′: 4,5]pyrazino[1,2-a]pyrimidine-9-carboxamide (5 mg, 78%) as an off-white solid. ¹H NMR (400 MHz, CDCl3) δ 0.74 (d, J=6.4 Hz, 3H), 0.84 (d, J=6.4 Hz, 3H), 0.97-1.00 (m, 6^(H),) 1.37-1.66 (m, 5H ), 1.75-1.82 (m, 1H), 2.05-2.09 (m, 2H), 2.21-2.26 (m, 1H), 2.72-2.70 (m, 1H), 2.87-2.93 (m, 1H), 4.16-4.26 (m, 2H), 4.38 (m, 1H), 4.56-4.66 (m, 2H), 4.93-4.99 (m, 1H), 6.97-7.02 (m, 2H), 7.31-7.34 (m, 2H), 8.27 (s, 1H), 10.49 (m, 1H), 12.61 (s, 1H); ES⁺ MS; 4.09 (M+1).

EXAMPLE ZZ-1 to ZZ-24

Examples in table below were isolated as a mixture of diastereomers ranging from 1:1 to >10:1 ratios of stereoisomers at the center indicated as undefined. Characterization data reported herein consists of observed mass spectral signals for molecular ions (M+1) of the compounds using electrospray ionization methods in the positive mode using LC/MS techniques well known in the field. Reported retention times refer to observed UV peaks confirmed by NMR methods for the examples below using the following gradient on a phenomenex C18 reverse phase HPLC column (150 mm×4.6 mm 5 micron). Solvent A=water w/0.1% formic acid, solvent B=acetonitrile w/0.1% formic acid. Gradient=10% B for 1 min, gradient from 10% to 90% B from 1 to 9 min, ramping to 100% B at 9.01 min and holding at 100% 13 for 2 min. In several cases the diastereomers were not separable by the standard HPLC conditions reported above and thus reported as a single retention time.

TABLE A Example Observed LC/MS or No. Structure HPLC data ZZ-1

ES⁺ MS: 419 (M + 1) ZZ-2

ES⁺ MS: 406 (M + 1) ZZ-3

ES⁺ MS: 509 (M + 1) ZZ-4

ES⁺ MS: 429 (M + 1) ZZ-5

ES⁺ MS: 415 (M + 1) ZZ-6

ES⁺ MS: 491 (M + 1) ZZ-7

ES⁺ MS: 509 (M + 1) ZZ-8

ES⁺ MS: 443 (M + 1) ZZ-9

ES⁺ MS: 461 (M + 1) ZZ-10

ES⁺ MS: 501 (M + 1) ZZ-11

ES⁺ MS: 475 (M + 1) ZZ-12

ES⁺ MS: 489 (M + 1) ZZ-13

ES⁺ MS: 460 (M + 1) ZZ-14

ES⁺ MS: 442 (M + 1) ZZ- 15

ES⁺ MS: 489 (M + 1) ZZ-16

8.174 & 8.295 min. ZZ-17

ES⁺ MS: 461 (M + 1) ZZ-18

ES⁺ MS: 447 (M + 1 ZZ-19

ES⁺ MS: 446 (M + 1) ZZ-20

ES⁺ MS: 432 (M + 1) ZZ-21

7.368 min ZZ-22

7.150 min ZZ-23

ES⁺ MS: 447 (M + 1) ZZ-24

ES⁺ MS: 447 (M + 1)

The present invention further includes the following compounds.

TABLE B No (R) m R^(a) 1 4-F —CH₃ 2 4-F —CH(CH₃)₂ 3 4-F —CH₂CH₂OCH₃ 4 2,4-F —CH₃ 5 2,4-F —CH(CH₃)₂ 6 2,4-F —CH₂CH₂OCH₃ 7 2-F, 3-Cl —CH₃ 8 2-F, 3-Cl —CH(CH₃)₂ 9 2-F, 3-Cl —CH₂CH₂OCH₃

EXPERIMENTAL EXAMPLE 1

The HIV integrase inhibitory activity was investigated based on the following assay method.

(1) Preparation of DNA Solution

By the same method as that described in Experimental Example 1 of WO 2004/024693, a substrate DNA solution (2 pmol/pl) and a target DNA solution (5 pmol/μl) were prepared. After each target DNA solution was once boiled, a temperature was slowly lowered to anneal complementary chains, which was used. Each sequence of a substrate DNA and a target DNA is as described in the same Experimental Example.

(2) Measurement of Inhibition Rate (IC₅₀ Value)

Streptavidin (manufactured by Vector Laboratories) was dissolved in a 0.1M carbonate buffer solution (composition: 90 mM Na₂CO₃, 10 mM NaHCO₃) to a concentration of 40 μg/ml. Each 50 μl of this solution was added to a well of an immunoplate (manufactured by NUNC), this is .allowed to stand at 4° C. overnight to adsorb. Then, each well was washed with a phosphate buffer (composition: 13.7 mM NaCl, 0.27 mM KCl, 0.43 mM Na₂HPO₄, 0.14 mM KH₂PO₄) two times, and 300 μl of a phosphate buffer containing 1% skim milk to block it for 30 minutes. Further, each well was washed with a phosphate buffer two times, 50 μl of a substrate DNA solution (2 pmol/μl ) was added to adsorb at room temperature for 30 minutes while shaking, and this was washed with a phosphate buffer two times and, then, distilled water once.

Then, to each well prepared as described above were added 12 μl of a buffer (composition: 150 mM MOPS (pH7.2), 75 mM MnCl₂, 50 mM 2-mercaptoethanol, 25% glycerol, 500 μg/ml bovine serum albumin-fraction V), and 51 μl of a reaction solution prepared from 39 μl of distilled water. Then, 9 μl of an integrase solution (30 pmol) was added, and the mixture was mixed well. To a well as a negative control (NC) was added 9 μl of a diluting solution (composition: 20 mM MOPS (pH7.2), 400 mM potassium glutamete, 1 mM EDTA, 0.1% NP-40, 20% glycerol, 1 mM DTT, 4 M urea), and this was mixed well using a plate mixer.

After the plate was incubated at 30° C. for 60 minutes, the reaction solution was discarded, followed by washing with 250 μl of a washing buffer (composition: 150 mM MOPS (pH7.2), 50 mM 2-mercaptoethanol, 25% glycerol, 500 μg/ml bovine serum albumin-fraction V) three times.

Then, to each well were added 12 μl of a buffer (composition: 150 mM MOPS (pH7.2), 75 mM MgCl_(2,) 50 mM 2-mercaptoethanol, 25% glycerol, 500 μg/ml bovine serum albumin-fraction V), and 53 μl of a reaction solution prepared from 41 μl of distilled water. Further, 6 μl of a solution of a test compound in DMSO was added to each well, and 6 μl of DMSO was added to a well as a positive control (PC), followed by mixing well using a plate mixer. After the plate was incubated at 30° C. for 30 minutes, 1 μl of a target DNA (5 pmol/μl) was added, and this was mixed well using a plate mixer.

After each plate was incubated at 30° C. for 10 minutes, the reaction solution was discarded, followed by washing with a phosphate buffer two times. Then, an anti-digoxigenin antibody labeled with alkaline phosphatase (sheep Fab fragment: manufactured by Boehringer) was diluted 2000-fold with an antibody diluting solution, 100 μl of the diluent was added to bind at 30° C. for 1 hour, and this was washed successively with a phosphate buffer containing 0.05% Tween20 two times, and a phosphate buffer once. Then, 150 μl of an alkaline phosphatase coloring buffer (composition: 10 mM paranitrophenyl phosphate (manufactured by Vector Laboratories), 5 mM MgCl₂, 100 mM NaCl, 100 mM Tris-HCl (pH 9.5)) was added to react at 30° C. for 2 hours, 50 μl of a 1N NaOH solution was added to stop the reaction, an absorbance (O D405 nm) of each well was measured, and an inhibition rate (IC₅₀) was obtained according to the following calculation equation.

Inhibition rate(%)=100[1−{(C abs.−NC abs.)/(PC abs.−NC abs.)}]

C abs.; absorbance of well of compound

NC abs.: absorbance of NC

PC abs.; absorbance of PC

Results are shown below.

TABLE 1 Integrase inhibitory activity Example No. (IC50, ng/ml) C-2 3.3 F-2 3.8 H-2 3.2

The present compound showed the strong integrase inhibitory activity against HIV.

EXPERIMENTAL EXAMPLE 2

A derivative of 293T cells expressing an attachment factor to improve adherence to plastic were used for the assay. A VSV-g pseudotyped HIV vector that expresses luciferase (herein referred to as PHIV) was produced by transfection of cells with the pGJ3-Luci vector plasmid (Jarmy, G, et al., J. Medical Virology, 64:223-231, 2001) and pVSV-g (Clontech). Cells were mixed with the PHIV vector and then mixed with serially diluted compounds. After incubation at 37° C. and 5% CO₂ for two days, the plates were read by using Steady Glo luciferase assay reagent (Promega) as recommended by the manufacturer. To assess non-HIV specific inhibition, a similar assay was performed, except that cell/PHIV vector mixture was replaced by cells which had been previously transduced and constitutively expressed luciferase.

TABLE 2 PHIV IC₅₀ * = <10 nM, Example ** = 10-100 nM, number *** >100 nM Z-1 * Z-2 * Z-3 * Z-4 * Z-5 * Z-6 * Z-7 * Z-8 ** Z-9 * Z-10 * Z-11 * Z-12 * Z-13 ** Z-14 ** Z-15 * Z-16 * Z-17 * Z-18 * Z-19 * Z-20 ** Z-21 * Z-22 * Z-23 * Z-24 * Z-25 * Z-26 * Z-27 *** Z-28 * Z-29 * Z-30 * Z-31 * Z-32 * Z-33 * Z-34 * Z-35 * Z-36 * Z-37 * Z-38 ** Z-39 * Z-40 * Z-41 * Z-42 * Z-43 * Z-44 * Z-45 * Z-46 * Z-47 * Z-48 * Z-49 * Z-50 * Z-51 * Z-52 * Z-53 * Z-54 * Z-55 ** Z-59 * Z-60 *

FORMULATION EXAMPLE

A term “active ingredient” means the present compound, a tautomer thereof, a pharmaceutically acceptable thereof, or a solvate thereof.

FORMULATION EXAMPLE 1

A hard gelatin capsule is prepared using the following ingredients:

dose (mg/capsule) Active ingredient 250 Starch (dried) 200 Magnesium stearate 10 Total 460 mg

FORMULATION EXAMPLE 2

A tablet is prepared using the following ingredients:

dose (mg/tablet) Active ingredient 250 Cellulose (microcrystalline) 400 Silicon dioxide (fumed) 10 Stearic acid 5 Total 665 mg

Ingredients are mixed, and compressed to obtain tablets, each weighing 665 mg. 

1-56. (canceled)
 57. A compound which is (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-c]pyrido[1,2-d]pyrazine-8-carboxamide or a pharmaceutically acceptable salt thereof.
 58. A compound which is (3R,11aS)-N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3]oxazolo[3,2-c]pyrido[1,2-d]pyrazine-8-carboxamide sodium salt.
 59. A compound of the formula:


60. A compound which is N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3 ]oxazolo[3,2-c]pyrido[1,2-d]pyrazine-8-carboxamide or a pharmaceutically acceptable salt thereof.
 61. A compound which is N-[(2,4-Difluorophenyl)methyl]-6-hydroxy-3-methyl-5,7-dioxo-2,3,5,7,11,11a-hexahydro[1,3 ]oxazolo[3,2-c]pyrido[1,2-d]pyrazine-8-carboxamide sodium salt.
 62. A compound of the formula:


63. A pharmaceutical composition comprising the compound of claim 57 or a pharmaceutically acceptable salt thereof together with a pharmaceutically acceptable carrier or diluent.
 64. A pharmaceutical composition comprising the compound of claim 58 together with a pharmaceutically acceptable carrier or diluent.
 65. A pharmaceutical composition comprising the compound of claim 59 or a pharmaceutically acceptable salt thereof together with a pharmaceutically acceptable carrier or diluent.
 66. A pharmaceutical composition comprising the compound of claim 60 or a pharmaceutically acceptable salt thereof together with a pharmaceutically acceptable carrier or diluent.
 67. A pharmaceutical composition comprising the compound of claim 61 together with a pharmaceutically acceptable carrier or diluent.
 68. A pharmaceutical composition comprising the compound of claim 62 or a pharmaceutically acceptable salt thereof together with a pharmaceutically acceptable carrier or diluent. 